ID HELS_SACS2 Reviewed; 708 AA.
AC Q97VY9;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
DE EC=5.6.2.4 {ECO:0000255|HAMAP-Rule:MF_00442};
DE AltName: Full=DNA 3'-5' helicase Hel308 {ECO:0000255|HAMAP-Rule:MF_00442};
GN Name=hel308 {ECO:0000255|HAMAP-Rule:MF_00442}; OrderedLocusNames=SSO2462;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000255|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00442}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00442}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK42601.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE006641; AAK42601.1; ALT_INIT; Genomic_DNA.
DR PIR; B90418; B90418.
DR RefSeq; WP_010923869.1; NC_002754.1.
DR AlphaFoldDB; Q97VY9; -.
DR SMR; Q97VY9; -.
DR STRING; 273057.SSO2462; -.
DR PaxDb; 273057-SSO2462; -.
DR EnsemblBacteria; AAK42601; AAK42601; SSO2462.
DR GeneID; 7806244; -.
DR KEGG; sso:SSO2462; -.
DR PATRIC; fig|273057.12.peg.2540; -.
DR eggNOG; arCOG00553; Archaea.
DR HOGENOM; CLU_006553_3_0_2; -.
DR InParanoid; Q97VY9; -.
DR PhylomeDB; Q97VY9; -.
DR EvolutionaryTrace; Q97VY9; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd18028; DEXHc_archSki2; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR Gene3D; 1.10.3380.30; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR048772; Hel308-like_dom4.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; NF040935; helicase_Hel308; 1.
DR PANTHER; PTHR47961:SF10; ATP-DEPENDENT DNA HELICASE HEL308; 1.
DR PANTHER; PTHR47961; DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21280; Helicase_dom4_arc; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF158702; Sec63 N-terminal domain-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW Isomerase; Nucleotide-binding; Reference proteome.
FT CHAIN 1..708
FT /note="ATP-dependent DNA helicase Hel308"
FT /id="PRO_0000102112"
FT DOMAIN 33..196
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT DOMAIN 229..435
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT MOTIF 1..29
FT /note="Q motif"
FT MOTIF 145..148
FT /note="DEAH box"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT BINDING 28
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00442"
SQ SEQUENCE 708 AA; 80500 MW; D52C24C89541C780 CRC64;
MSIDDLKLPS NVIDIIKNRG IKKLNPPQTE AVKKGLLDGN RLLLTSPTGS GKTLIAEMGI
ISFLLKNGGK AIYVTPLRAL TNEKYLTFKD WESIGFKVAM TSGDYDTDDA WLKNYDIIIT
TYEKLDSLWR HRPDWLNEAN YFVLDELHYL NDPERGPVVE SVTIRAKRRN LLALSATISN
YKQIAKWLGA EPVATNWRPV PLMEGVMYPE RKKKEYTILF RDNTARKVHG DDAIIAYTLD
SLSKNGQVLV FRNSRKMAES TALKIANYMN FVSLDEKAIS EILKQLDDIE EGGSDEKELL
KSLISKGVAY HHAGLSKALR DIIEESFRKR KIKVIVATPT LAAGVNLPAR TVIIGDIYRF
NRKIVGYYDE IPVMEYKQMS GRAGRPGFDQ IGESIIVVRD KEDVDRVFKK YILSDVEPIE
SKLGSERAFY TFLLGILSAE GSLSEKQLEA FAYESLLAKS VVDVYFDRAI RWLSEHSFIR
EENNTFTLTN FGKRVADLYI NPFTADIIRK GLEGYKASCE IAYLHLLAFT PDGPLVSVGR
NEEEELIELL EDLECELLVE EPYEEDEYSL YLNALKVALI MKDWIDEVDE DTILGKYNIG
SGDLRNIVET MDWLTYSAYH LSKELRLDDH SDKLRILNLR VTDGVKEELL ELVQIGGVGR
KRARLLYNNG IKGLGDVVMN PDRVRNLLGQ KLGERVVQEA ARLLNRFH
//
