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Database: UniProt
Entry: Q98SK0
LinkDB: Q98SK0
Original site: Q98SK0 
ID   MDFIC_XENLA             Reviewed;         246 AA.
AC   Q98SK0;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   16-JAN-2019, entry version 48.
DE   RecName: Full=MyoD family inhibitor domain-containing protein;
DE   AltName: Full=I-mfa domain-containing protein;
DE   AltName: Full=XIC;
GN   Name=mdfic {ECO:0000250|UniProtKB:Q9P1T7};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus;
OC   Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAG54082.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   TISSUE=Oocyte {ECO:0000269|PubMed:11238923};
RX   PubMed=11238923; DOI=10.1128/MCB.21.5.1866-1873.2001;
RA   Snider L., Thirlwell H., Miller J.R., Moon R.T., Groudine M.,
RA   Tapscott S.J.;
RT   "Inhibition of Tcf3 binding by I-mfa domain proteins.";
RL   Mol. Cell. Biol. 21:1866-1873(2001).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=15923623; DOI=10.1128/MCB.25.12.5061-5072.2005;
RA   Snider L., Tapscott S.J.;
RT   "XIC is required for Siamois activity and dorsoanterior development.";
RL   Mol. Cell. Biol. 25:5061-5072(2005).
CC   -!- FUNCTION: May act as a transcriptional activator or repressor.
CC       Inhibits the transcriptional activation of Zic family proteins.
CC       Retains nuclear Zic proteins in the cytoplasm (By similarity).
CC       Required for dorsoanterior development. Necessary for siamois to
CC       activate downstream target genes, including gsc, during execution
CC       of the dorsal organizer program. Also regulates the
CC       transcriptional activity of TCF7L1/TCF3 by interacting directly
CC       with TCF7L1/TCF3 and preventing it from binding DNA. {ECO:0000250,
CC       ECO:0000269|PubMed:11238923, ECO:0000269|PubMed:15923623}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9P1T7}.
CC       Nucleus {ECO:0000250|UniProtKB:Q9P1T7}. Nucleus, nucleolus
CC       {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed broadly at a low level in the early
CC       embryo. {ECO:0000269|PubMed:11238923,
CC       ECO:0000269|PubMed:15923623}.
CC   -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically,
CC       with expression persisting to at least tadpole stages.
CC       {ECO:0000269|PubMed:11238923}.
CC   -!- DOMAIN: The C2H2-type 3, 4 and 5 zinc finger domains are necessary
CC       for transcription activation. The cysteine-rich C-terminus is
CC       involved in its granular distribution in the cytoplasm (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MDFI family. {ECO:0000255}.
DR   EMBL; AY017419; AAG54082.1; -; mRNA.
DR   RefSeq; NP_001082078.1; NM_001088609.1.
DR   UniGene; Xl.757; -.
DR   SMR; Q98SK0; -.
DR   BioGrid; 99548; 1.
DR   GeneID; 398213; -.
DR   KEGG; xla:398213; -.
DR   CTD; 398213; -.
DR   Xenbase; XB-GENE-866365; mdfic.
DR   HOVERGEN; HBG068212; -.
DR   OrthoDB; 1487193at2759; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0008134; F:transcription factor binding; IPI:UniProtKB.
DR   GO; GO:0009950; P:dorsal/ventral axis specification; IMP:UniProtKB.
DR   GO; GO:0043392; P:negative regulation of DNA binding; IDA:UniProtKB.
DR   GO; GO:0042308; P:negative regulation of protein import into nucleus; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IMP:UniProtKB.
DR   InterPro; IPR026134; MDFI/MDFIC.
DR   PANTHER; PTHR15304; PTHR15304; 1.
DR   Pfam; PF15316; MDFI; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; Developmental protein; Nucleus; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN         1    246       MyoD family inhibitor domain-containing
FT                                protein.
FT                                /FTId=PRO_0000280224.
FT   DOMAIN       74    246       MDFI.
FT   COMPBIAS    167    243       Cys-rich. {ECO:0000255}.
SQ   SEQUENCE   246 AA;  26766 MW;  377B9E79A70F765A CRC64;
     MSQEREPFSP RPEGPEQVTS TEESLLVPLP HGKCEKENMD LEDTTLYRNK SIQTDIEDRS
     ILEDNSNSQP IKAQPQRLPQ PNTSALEQSE EETGKIQNGH VGLSNINGIH NGVKHVPADH
     RNISAPVSQK MHRKIQSSLS VSSDGSKKSK ESSAYSQKPS ASPEDGCVHC ILTCLFCEFL
     TLCNIVVGQA SCGICTSEAC CCCCTEEMGD DCNCPCDMDC GIMDACCESS DCLEICMECC
     GICFPS
//
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