ID LIMD_DICDI Reviewed; 198 AA.
AC Q9BIW4; Q54K92;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 24-JAN-2024, entry version 99.
DE RecName: Full=LIM domain-containing protein D;
GN Name=limD; Synonyms=limD1; ORFNames=DDB_G0287507;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=12374734; DOI=10.1093/emboj/cdf550;
RA Khurana B., Khurana T., Khaire N., Noegel A.A.;
RT "Functions of LIM proteins in cell polarity and chemotactic motility.";
RL EMBO J. 21:5331-5342(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Binds to F-actin and may modulate the chemotactic response
CC during early development and contribute to the maintenance of the
CC strength of the actin cytoskeleton. {ECO:0000269|PubMed:12374734}.
CC -!- SUBCELLULAR LOCATION: Cell projection, pseudopodium
CC {ECO:0000269|PubMed:12374734}. Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:12374734}. Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:12374734}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the growth phase and early
CC development. Levels start to decrease strongly after tight aggregates
CC formation. {ECO:0000269|PubMed:12374734}.
CC -!- DISRUPTION PHENOTYPE: Cells exhibit normal growth, but display altered
CC morphology and F-actin distribution. They show changes in chemotactic
CC motility associated with increased pseudopod formation, are
CC significantly impaired in growth under stress conditions and highly
CC sensitive to osmotic shock. {ECO:0000269|PubMed:12374734}.
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DR EMBL; AF348467; AAK30153.1; -; Genomic_DNA.
DR EMBL; AAFI02000102; EAL63653.1; -; Genomic_DNA.
DR RefSeq; XP_637163.1; XM_632071.1.
DR AlphaFoldDB; Q9BIW4; -.
DR SMR; Q9BIW4; -.
DR STRING; 44689.Q9BIW4; -.
DR PaxDb; 44689-DDB0191401; -.
DR EnsemblProtists; EAL63653; EAL63653; DDB_G0287507.
DR GeneID; 8626164; -.
DR KEGG; ddi:DDB_G0287507; -.
DR dictyBase; DDB_G0287507; limD1.
DR eggNOG; KOG1700; Eukaryota.
DR HOGENOM; CLU_1380339_0_0_1; -.
DR InParanoid; Q9BIW4; -.
DR OMA; CANHYPV; -.
DR PhylomeDB; Q9BIW4; -.
DR Reactome; R-DDI-983231; Factors involved in megakaryocyte development and platelet production.
DR PRO; PR:Q9BIW4; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0031941; C:filamentous actin; IDA:dictyBase.
DR GO; GO:0044354; C:macropinosome; IDA:dictyBase.
DR GO; GO:0001891; C:phagocytic cup; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0031143; C:pseudopodium; IDA:dictyBase.
DR GO; GO:0051015; F:actin filament binding; IDA:dictyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051017; P:actin filament bundle assembly; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IMP:dictyBase.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; TAS:dictyBase.
DR GO; GO:0030010; P:establishment of cell polarity; IMP:dictyBase.
DR GO; GO:0006970; P:response to osmotic stress; IMP:dictyBase.
DR CDD; cd09358; LIM_Mical_like; 1.
DR Gene3D; 2.10.110.10; Cysteine Rich Protein; 1.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24206:SF64; LIM DOMAIN-CONTAINING PROTEIN D; 1.
DR PANTHER; PTHR24206; OS06G0237300 PROTEIN; 1.
DR Pfam; PF00412; LIM; 1.
DR SMART; SM00132; LIM; 1.
DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 2.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 1.
PE 2: Evidence at transcript level;
KW Actin-binding; Cell projection; Chemotaxis; Cytoplasm; Cytoskeleton;
KW LIM domain; Metal-binding; Reference proteome; Repeat; Zinc.
FT CHAIN 1..198
FT /note="LIM domain-containing protein D"
FT /id="PRO_0000328167"
FT DOMAIN 5..65
FT /note="LIM zinc-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
SQ SEQUENCE 198 AA; 21501 MW; B2FB8E6A66387969 CRC64;
MSNLGKCTRC QKTVYSQEGF IAVKVPFHRS CFKCEVCNWQ LVLTNYKSIN GKVYCANHYP
VGGLSVTPEK THTTSDDLVM KNALNAPRVE TVNEQLRGGN EKPQTSTDDL VTKNALNAPK
SNLVNNQVRG TSDSAPLTSA DDLVTRNALK APKSDLVNNQ VRGTSEMGPQ AGIDIVTANA
LKAPKLETMS GYQKSVQN
//
