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Database: UniProt
Entry: Q9BZD6
LinkDB: Q9BZD6
Original site: Q9BZD6 
ID   TMG4_HUMAN              Reviewed;         226 AA.
AC   Q9BZD6;
DT   31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   18-SEP-2019, entry version 147.
DE   RecName: Full=Transmembrane gamma-carboxyglutamic acid protein 4 {ECO:0000305};
DE   AltName: Full=Proline-rich gamma-carboxyglutamic acid protein 4;
DE            Short=Proline-rich Gla protein 4;
DE   Flags: Precursor;
GN   Name=PRRG4 {ECO:0000303|PubMed:28859078, ECO:0000312|HGNC:HGNC:30799};
GN   Synonyms=PRGP4, TMG4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=11171957; DOI=10.1073/pnas.98.4.1370;
RA   Kulman J.D., Harris J.E., Xie L., Davie E.W.;
RT   "Identification of two novel transmembrane gamma-carboxyglutamic acid
RT   proteins expressed broadly in fetal and adult tissues.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:1370-1375(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D.,
RA   Wang L., Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human
RT   liver phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [4]
RP   FUNCTION.
RX   PubMed=28859078; DOI=10.1371/journal.pgen.1006865;
RA   Justice E.D., Barnum S.J., Kidd T.;
RT   "The WAGR syndrome gene PRRG4 is a functional homologue of the
RT   commissureless axon guidance gene.";
RL   PLoS Genet. 13:E1006865-E1006865(2017).
CC   -!- FUNCTION: May control axon guidance across the CNS
CC       (PubMed:28859078). Prevents the delivery of ROBO1 at the cell
CC       surface and downregulates its expression (PubMed:28859078).
CC       {ECO:0000269|PubMed:28859078}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum-Golgi intermediate
CC       compartment membrane {ECO:0000269|PubMed:28859078}; Single-pass
CC       membrane protein {ECO:0000305|PubMed:28859078}.
CC   -!- TISSUE SPECIFICITY: Expressed in lung, liver, kidney, pancreas and
CC       placenta. {ECO:0000269|PubMed:11171957}.
CC   -!- PTM: Gla residues are produced after subsequent post-translational
CC       modifications of glutamate by a vitamin K-dependent gamma-
CC       carboxylase.
CC   -!- SIMILARITY: Belongs to the commissureless family. {ECO:0000305}.
DR   EMBL; AF326351; AAK00956.1; -; mRNA.
DR   EMBL; BC010052; AAH10052.1; -; mRNA.
DR   EMBL; BC063393; AAH63393.1; -; mRNA.
DR   CCDS; CCDS7881.1; -.
DR   RefSeq; NP_076986.1; NM_024081.5.
DR   RefSeq; XP_006718377.1; XM_006718314.3.
DR   SMR; Q9BZD6; -.
DR   BioGrid; 122514; 9.
DR   IntAct; Q9BZD6; 9.
DR   STRING; 9606.ENSP00000257836; -.
DR   iPTMnet; Q9BZD6; -.
DR   PhosphoSitePlus; Q9BZD6; -.
DR   BioMuta; PRRG4; -.
DR   DMDM; 20140760; -.
DR   EPD; Q9BZD6; -.
DR   jPOST; Q9BZD6; -.
DR   MassIVE; Q9BZD6; -.
DR   MaxQB; Q9BZD6; -.
DR   PaxDb; Q9BZD6; -.
DR   PeptideAtlas; Q9BZD6; -.
DR   PRIDE; Q9BZD6; -.
DR   ProteomicsDB; 79816; -.
DR   DNASU; 79056; -.
DR   Ensembl; ENST00000257836; ENSP00000257836; ENSG00000135378.
DR   GeneID; 79056; -.
DR   KEGG; hsa:79056; -.
DR   UCSC; uc001mtx.4; human.
DR   CTD; 79056; -.
DR   DisGeNET; 79056; -.
DR   GeneCards; PRRG4; -.
DR   HGNC; HGNC:30799; PRRG4.
DR   HPA; HPA009040; -.
DR   HPA; HPA024566; -.
DR   MIM; 611690; gene.
DR   neXtProt; NX_Q9BZD6; -.
DR   OpenTargets; ENSG00000135378; -.
DR   PharmGKB; PA134974592; -.
DR   eggNOG; ENOG410IMYY; Eukaryota.
DR   eggNOG; ENOG410YJW1; LUCA.
DR   GeneTree; ENSGT00940000158268; -.
DR   HOGENOM; HOG000115997; -.
DR   InParanoid; Q9BZD6; -.
DR   OMA; AFPHCTR; -.
DR   OrthoDB; 1354567at2759; -.
DR   PhylomeDB; Q9BZD6; -.
DR   TreeFam; TF332123; -.
DR   ChiTaRS; PRRG4; human.
DR   GenomeRNAi; 79056; -.
DR   Pharos; Q9BZD6; -.
DR   PRO; PR:Q9BZD6; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   Bgee; ENSG00000135378; Expressed in 186 organ(s), highest expression level in mouth mucosa.
DR   ExpressionAtlas; Q9BZD6; baseline and differential.
DR   Genevisible; Q9BZD6; HS.
DR   GO; GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   Gene3D; 4.10.740.10; -; 1.
DR   InterPro; IPR017857; Coagulation_fac-like_Gla_dom.
DR   InterPro; IPR035972; GLA-like_dom_SF.
DR   InterPro; IPR000294; GLA_domain.
DR   Pfam; PF00594; Gla; 1.
DR   PRINTS; PR00001; GLABLOOD.
DR   SMART; SM00069; GLA; 1.
DR   SUPFAM; SSF57630; SSF57630; 1.
DR   PROSITE; PS00011; GLA_1; 1.
DR   PROSITE; PS50998; GLA_2; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Complete proteome; Disulfide bond;
KW   Gamma-carboxyglutamic acid; Membrane; Phosphoprotein; Polymorphism;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL        1     17       {ECO:0000255}.
FT   PROPEP       18     49       {ECO:0000255}.
FT                                /FTId=PRO_0000022551.
FT   CHAIN        50    226       Transmembrane gamma-carboxyglutamic acid
FT                                protein 4.
FT                                /FTId=PRO_0000022552.
FT   TOPO_DOM     50    113       Extracellular. {ECO:0000255}.
FT   TRANSMEM    114    134       Helical. {ECO:0000255}.
FT   TOPO_DOM    135    226       Cytoplasmic. {ECO:0000255}.
FT   DOMAIN       52     98       Gla. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   COMPBIAS    203    208       Poly-Pro.
FT   MOD_RES      58     58       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      66     66       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      68     68       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      71     71       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      72     72       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      77     77       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      78     78       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      81     81       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES      86     86       4-carboxyglutamate. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00463}.
FT   MOD_RES     163    163       Phosphoserine.
FT                                {ECO:0000244|PubMed:24275569}.
FT   DISULFID     69     74       {ECO:0000255|PROSITE-ProRule:PRU00463}.
FT   VARIANT      33     33       E -> K (in dbSNP:rs33962176).
FT                                /FTId=VAR_051443.
FT   VARIANT     143    143       C -> R (in dbSNP:rs34736080).
FT                                /FTId=VAR_051444.
FT   VARIANT     176    176       P -> Q (in dbSNP:rs34139105).
FT                                /FTId=VAR_051445.
SQ   SEQUENCE   226 AA;  25403 MW;  45C783E3825797EE CRC64;
     MFTLLVLLSQ LPTVTLGFPH CARGPKASKH AGEEVFTSKE EANFFIHRRL LYNRFDLELF
     TPGNLERECN EELCNYEEAR EIFVDEDKTI AFWQEYSAKG PTTKSDGNRE KIDVMGLLTG
     LIAAGVFLVI FGLLGYYLCI TKCNRLQHPC SSAVYERGRH TPSIIFRRPE EAALSPLPPS
     VEDAGLPSYE QAVALTRKHS VSPPPPYPGH TKGFRVFKKS MSLPSH
//
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