GenomeNet

Database: UniProt
Entry: Q9CM00
LinkDB: Q9CM00
Original site: Q9CM00 
ID   GSHAB_PASMU             Reviewed;         757 AA.
AC   Q9CM00;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   13-FEB-2019, entry version 106.
DE   RecName: Full=Glutathione biosynthesis bifunctional protein GshAB {ECO:0000255|HAMAP-Rule:MF_00782};
DE   AltName: Full=Gamma-GCS-GS {ECO:0000255|HAMAP-Rule:MF_00782};
DE            Short=GCS-GS {ECO:0000255|HAMAP-Rule:MF_00782};
DE   Includes:
DE     RecName: Full=Glutamate--cysteine ligase {ECO:0000255|HAMAP-Rule:MF_00782};
DE              EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_00782};
DE     AltName: Full=Gamma-ECS {ECO:0000255|HAMAP-Rule:MF_00782};
DE              Short=GCS {ECO:0000255|HAMAP-Rule:MF_00782};
DE     AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000255|HAMAP-Rule:MF_00782};
DE   Includes:
DE     RecName: Full=Glutathione synthetase {ECO:0000255|HAMAP-Rule:MF_00782};
DE              EC=6.3.2.3 {ECO:0000255|HAMAP-Rule:MF_00782};
DE     AltName: Full=GSH synthetase {ECO:0000255|HAMAP-Rule:MF_00782};
DE              Short=GS {ECO:0000255|HAMAP-Rule:MF_00782};
DE              Short=GSH-S {ECO:0000255|HAMAP-Rule:MF_00782};
DE              Short=GSHase {ECO:0000255|HAMAP-Rule:MF_00782};
DE     AltName: Full=Glutathione synthase {ECO:0000255|HAMAP-Rule:MF_00782};
GN   Name=gshAB {ECO:0000255|HAMAP-Rule:MF_00782};
GN   Synonyms=gshF {ECO:0000255|HAMAP-Rule:MF_00782};
GN   OrderedLocusNames=PM1048;
OS   Pasteurella multocida (strain Pm70).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Pasteurella.
OX   NCBI_TaxID=272843;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pm70;
RX   PubMed=11248100; DOI=10.1073/pnas.051634598;
RA   May B.J., Zhang Q., Li L.L., Paustian M.L., Whittam T.S., Kapur V.;
RT   "Complete genomic sequence of Pasteurella multocida Pm70.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3460-3465(2001).
CC   -!- FUNCTION: Synthesizes glutathione from L-glutamate and L-cysteine
CC       via gamma-L-glutamyl-L-cysteine. {ECO:0000255|HAMAP-
CC       Rule:MF_00782}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + H(+) + L-gamma-
CC         glutamyl-L-cysteine + phosphate; Xref=Rhea:RHEA:13285,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:35235, ChEBI:CHEBI:43474, ChEBI:CHEBI:58173,
CC         ChEBI:CHEBI:456216; EC=6.3.2.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00782};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + L-gamma-glutamyl-L-cysteine = ADP +
CC         glutathione + H(+) + phosphate; Xref=Rhea:RHEA:13557,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57925, ChEBI:CHEBI:58173,
CC         ChEBI:CHEBI:456216; EC=6.3.2.3; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00782};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium or manganese ions per subunit.
CC       {ECO:0000250};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 1/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00782}.
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00782}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00782}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the glutamate--
CC       cysteine ligase type 1 family. Type 2 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00782}.
DR   EMBL; AE004439; AAK03132.1; -; Genomic_DNA.
DR   RefSeq; WP_010906990.1; NC_002663.1.
DR   PDB; 3LN7; X-ray; 3.20 A; A/B=1-757.
DR   PDBsum; 3LN7; -.
DR   ProteinModelPortal; Q9CM00; -.
DR   SMR; Q9CM00; -.
DR   STRING; 272843.PM1048; -.
DR   EnsemblBacteria; AAK03132; AAK03132; PM1048.
DR   KEGG; pmu:PM1048; -.
DR   PATRIC; fig|272843.6.peg.1062; -.
DR   eggNOG; ENOG4105D9M; Bacteria.
DR   eggNOG; COG1181; LUCA.
DR   eggNOG; COG2918; LUCA.
DR   HOGENOM; HOG000156471; -.
DR   KO; K01919; -.
DR   OMA; EANFNPM; -.
DR   BioCyc; PMUL272843:G1FZ8-1110-MONOMER; -.
DR   SABIO-RK; Q9CM00; -.
DR   UniPathway; UPA00142; UER00209.
DR   UniPathway; UPA00142; UER00210.
DR   Proteomes; UP000000809; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   HAMAP; MF_00782; Glut_biosynth; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR003806; ATP-grasp_PylC-type.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR007370; Glu_cys_ligase.
DR   InterPro; IPR006335; Glut_biosynth.
DR   InterPro; IPR006334; Glut_cys_ligase.
DR   InterPro; IPR040657; GshAB_ATP-grasp.
DR   PANTHER; PTHR38761; PTHR38761; 2.
DR   Pfam; PF02655; ATP-grasp_3; 1.
DR   Pfam; PF18419; ATP-grasp_6; 1.
DR   Pfam; PF04262; Glu_cys_ligase; 2.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR01435; glu_cys_lig_rel; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Complete proteome;
KW   Glutathione biosynthesis; Ligase; Magnesium; Manganese; Metal-binding;
KW   Multifunctional enzyme; Nucleotide-binding; Reference proteome.
FT   CHAIN         1    757       Glutathione biosynthesis bifunctional
FT                                protein GshAB.
FT                                /FTId=PRO_0000192557.
FT   DOMAIN      494    753       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_00782}.
FT   NP_BIND     521    580       ATP. {ECO:0000255|HAMAP-Rule:MF_00782}.
FT   REGION        1    337       Glutamate--cysteine ligase.
FT   METAL       702    702       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_00782}.
FT   METAL       723    723       Magnesium or manganese 1.
FT                                {ECO:0000255|HAMAP-Rule:MF_00782}.
FT   METAL       723    723       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_00782}.
FT   METAL       725    725       Magnesium or manganese 2.
FT                                {ECO:0000255|HAMAP-Rule:MF_00782}.
FT   HELIX         5     10       {ECO:0000244|PDB:3LN7}.
FT   HELIX        13     17       {ECO:0000244|PDB:3LN7}.
FT   STRAND       19     28       {ECO:0000244|PDB:3LN7}.
FT   STRAND       34     36       {ECO:0000244|PDB:3LN7}.
FT   STRAND       43     45       {ECO:0000244|PDB:3LN7}.
FT   TURN         48     50       {ECO:0000244|PDB:3LN7}.
FT   STRAND       52     55       {ECO:0000244|PDB:3LN7}.
FT   STRAND       57     59       {ECO:0000244|PDB:3LN7}.
FT   STRAND       62     72       {ECO:0000244|PDB:3LN7}.
FT   HELIX        73     90       {ECO:0000244|PDB:3LN7}.
FT   STRAND      100    102       {ECO:0000244|PDB:3LN7}.
FT   TURN        109    111       {ECO:0000244|PDB:3LN7}.
FT   HELIX       120    133       {ECO:0000244|PDB:3LN7}.
FT   HELIX       136    139       {ECO:0000244|PDB:3LN7}.
FT   STRAND      144    149       {ECO:0000244|PDB:3LN7}.
FT   HELIX       151    160       {ECO:0000244|PDB:3LN7}.
FT   TURN        162    164       {ECO:0000244|PDB:3LN7}.
FT   HELIX       167    192       {ECO:0000244|PDB:3LN7}.
FT   TURN        204    206       {ECO:0000244|PDB:3LN7}.
FT   STRAND      217    219       {ECO:0000244|PDB:3LN7}.
FT   STRAND      222    225       {ECO:0000244|PDB:3LN7}.
FT   HELIX       239    251       {ECO:0000244|PDB:3LN7}.
FT   STRAND      256    258       {ECO:0000244|PDB:3LN7}.
FT   STRAND      264    269       {ECO:0000244|PDB:3LN7}.
FT   HELIX       273    275       {ECO:0000244|PDB:3LN7}.
FT   HELIX       276    279       {ECO:0000244|PDB:3LN7}.
FT   STRAND      283    286       {ECO:0000244|PDB:3LN7}.
FT   HELIX       301    316       {ECO:0000244|PDB:3LN7}.
FT   HELIX       323    338       {ECO:0000244|PDB:3LN7}.
FT   HELIX       348    364       {ECO:0000244|PDB:3LN7}.
FT   HELIX       369    383       {ECO:0000244|PDB:3LN7}.
FT   HELIX       385    387       {ECO:0000244|PDB:3LN7}.
FT   HELIX       389    399       {ECO:0000244|PDB:3LN7}.
FT   STRAND      400    402       {ECO:0000244|PDB:3LN7}.
FT   HELIX       403    419       {ECO:0000244|PDB:3LN7}.
FT   TURN        420    423       {ECO:0000244|PDB:3LN7}.
FT   TURN        427    430       {ECO:0000244|PDB:3LN7}.
FT   HELIX       433    445       {ECO:0000244|PDB:3LN7}.
FT   STRAND      448    453       {ECO:0000244|PDB:3LN7}.
FT   TURN        454    457       {ECO:0000244|PDB:3LN7}.
FT   STRAND      458    463       {ECO:0000244|PDB:3LN7}.
FT   STRAND      466    471       {ECO:0000244|PDB:3LN7}.
FT   TURN        472    474       {ECO:0000244|PDB:3LN7}.
FT   STRAND      477    481       {ECO:0000244|PDB:3LN7}.
FT   HELIX       482    489       {ECO:0000244|PDB:3LN7}.
FT   HELIX       491    500       {ECO:0000244|PDB:3LN7}.
FT   STRAND      507    511       {ECO:0000244|PDB:3LN7}.
FT   HELIX       513    518       {ECO:0000244|PDB:3LN7}.
FT   HELIX       519    522       {ECO:0000244|PDB:3LN7}.
FT   STRAND      523    526       {ECO:0000244|PDB:3LN7}.
FT   STRAND      528    534       {ECO:0000244|PDB:3LN7}.
FT   TURN        537    540       {ECO:0000244|PDB:3LN7}.
FT   HELIX       551    564       {ECO:0000244|PDB:3LN7}.
FT   STRAND      566    572       {ECO:0000244|PDB:3LN7}.
FT   STRAND      576    584       {ECO:0000244|PDB:3LN7}.
FT   STRAND      587    594       {ECO:0000244|PDB:3LN7}.
FT   STRAND      597    600       {ECO:0000244|PDB:3LN7}.
FT   HELIX       601    603       {ECO:0000244|PDB:3LN7}.
FT   HELIX       607    615       {ECO:0000244|PDB:3LN7}.
FT   STRAND      623    629       {ECO:0000244|PDB:3LN7}.
FT   HELIX       635    644       {ECO:0000244|PDB:3LN7}.
FT   STRAND      648    650       {ECO:0000244|PDB:3LN7}.
FT   STRAND      657    659       {ECO:0000244|PDB:3LN7}.
FT   HELIX       666    668       {ECO:0000244|PDB:3LN7}.
FT   STRAND      672    674       {ECO:0000244|PDB:3LN7}.
FT   TURN        676    678       {ECO:0000244|PDB:3LN7}.
FT   HELIX       681    694       {ECO:0000244|PDB:3LN7}.
FT   STRAND      697    706       {ECO:0000244|PDB:3LN7}.
FT   STRAND      708    710       {ECO:0000244|PDB:3LN7}.
FT   TURN        716    718       {ECO:0000244|PDB:3LN7}.
FT   STRAND      720    727       {ECO:0000244|PDB:3LN7}.
FT   HELIX       730    734       {ECO:0000244|PDB:3LN7}.
FT   STRAND      737    739       {ECO:0000244|PDB:3LN7}.
FT   HELIX       745    752       {ECO:0000244|PDB:3LN7}.
SQ   SEQUENCE   757 AA;  85862 MW;  CF0E49A661C14336 CRC64;
     MKIQHIIHEN QLGLLFQQGS FGLEKESQRV TADGAIVTTP HPAVFGNRRY HPYIQTDFAE
     SQLELITPPT KKLEDTFRWL SVIHEVVQRS LPEEEYIFPL SMPAGLPAEE QIRVAQLDNP
     EDVAYREYLV KIYGKNKQMV SGIHYNFQLS PDLITRLFRL QNEYQSAVDF QNDLYLKMAK
     NFLRYQWILL YLLAATPTVE SAYFKDGSPL AKGQFVRSLR SSQYGYVNDP EINVSFDSVE
     KYVESLEHWV STGKLIAEKE FYSNVRLRGA KKAREFLTTG IQYLEFRLFD LNPFEIYGIS
     LKDAKFIHVF ALFMIWMDHT ADQEEVELGK ARLAEVAFEH PLEKTAYAVE GELVLLELLS
     MLEQIGAEPE LFEIVKEKLT QFTDPSKTVA GRLVRAIEQA GSDQQLGAQL AQQYKAQAFE
     RFYALSAFDN MELSTQALLF DVIQKGIHTE ILDENDQFLC LKYGDHIEYV KNGNMTSHDS
     YISPLIMENK VVTKKVLQKA GFNVPQSVEF TSLEKAVASY ALFENRAVVI KPKSTNYGLG
     ITIFQQGVQN REDFAKALEI AFREDKEVMV EDYLVGTEYR FFVLGDETLA VLLRVPANVV
     GDSVHSVAEL VAMKNDHPLR GDGSRTPLKK IALGEIEQLQ LKEQGLTIDS IPAKDQLVQL
     RANSNISTGG DSIDMTDEMH ESYKQLAVGI TKAMGAAVCG VDLIIPDLKQ PATPNLTSWG
     VIEANFNPMM MMHIFPYAGK SRRLTQNVIK MLFPELE
//
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