ID Q9EMI5_AMEPV Unreviewed; 1301 AA.
AC Q9EMI5;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=DNA-directed RNA polymerase 147 kDa polypeptide {ECO:0000256|ARBA:ARBA00021673};
DE EC=2.7.7.6 {ECO:0000256|ARBA:ARBA00012418};
GN Name=AMV221 {ECO:0000313|EMBL:AAG02927.1};
OS Amsacta moorei entomopoxvirus (AmEPV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Entomopoxvirinae; Betaentomopoxvirus.
OX NCBI_TaxID=28321 {ECO:0000313|EMBL:AAG02927.1, ECO:0000313|Proteomes:UP000000872};
OH NCBI_TaxID=340055; Amsacta.
RN [1] {ECO:0000313|EMBL:AAG02927.1, ECO:0000313|Proteomes:UP000000872}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=10936094; DOI=10.1006/viro.2000.0449;
RA Bawden A.L., Glassberg K.J., Diggans J., Shaw R., Farmerie W., Moyer R.W.;
RT "Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis
RT and comparison with other poxviruses.";
RL Virology 274:120-139(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00024550};
CC -!- SIMILARITY: Belongs to the poxviridae DNA-directed RNA polymerase 147
CC kDa subunit family. {ECO:0000256|ARBA:ARBA00006884}.
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DR EMBL; AF250284; AAG02927.1; -; Genomic_DNA.
DR RefSeq; NP_065003.1; NC_002520.1.
DR GeneID; 1494811; -.
DR KEGG; vg:1494811; -.
DR Proteomes; UP000000872; Genome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000000872};
KW Transcription {ECO:0000256|ARBA:ARBA00023163};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 198..485
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
SQ SEQUENCE 1301 AA; 150299 MW; 3E7D097185975BB7 CRC64;
MTTFKYTLLD NSTIDAIPIV IDSIGNDNEN SVKSPKLGGT KFNVCSTCNL TRENGDMGHP
GRTPLRDMCI VKSGCIKNVL DTLNTLKLCN SCFMIKNNTI FSEIIEKYNS EYNINLKKEI
LSLLKNNRQG GVKCNNENCQ NITGTYKYNQ KKSYFYVKKQ KDEIILNKTV YTMLLGIPDI
IYKCVTVPYA DSQLQPYKAF YANNIIIPVL PSRPPNYFDN KESHVMTTKL GQLVGTSQKS
RDESEVQKIY NDIDNVKPNS PYKTSNMLVT LNIQVGGNKK GSIVRSNIMA RRADNTARCV
AGPTMDKIGY IYIPKIVAKT LTSSIYYNRF TENMIKDMLV NDNNKIKYIL LYRYDQLKPT
TLLKIKPQSR LNNLLKMKYG DRIEVELEDN DVILFSRQPS LHKFNIQAGI CKIWDNNTIA
TPTPIANSMN LDYDGDEMNV YKLKSSVSVE SLFTMLSVNM IKNNYNFSPI FGLIQDQIIS
VHMIYNIKEF SLQDVIYILG EYSYYIRDIN KKTYSGKELL SLLFPDNLTY EGMFDNGKIT
LSNISSKQVV AQSYESFSNI LSQLKNNIYA VYFIDVILYV ARNFINLYSF SVSLKDIIPD
IYFIDDVQEY INNCCKVIQY VALQYYIKKD HIIKLTYDEM ENIRIQNGNN IISNVKNKIN
NLFKDEKLNT IMMMKNSGYK ITLDELVTVL GCTGQQGIDS DDIPKPGIMG RVFDSTLPGS
LDIESLGYVK SSTIKGLKFE ELAFHTKYNS IKKILKITCE TSSAGSIGRK LVKFMEGVKV
DHLGRSVLNN DIIWYNTNHI KMTGGDISKV EILTPSLEMV NYTLIKEIYN ENKKYLLTNF
NTEINKEFIF PINIKLEIQS FYNKKSTPIS DIDALKLIDE FIEYVYINIY FYNITIDWFK
YILYTYLDRN TVEKYNKKYS KELLNYIINK IKLKLLNSLN PGYPIGLEYA NNIQEKFTQQ
SLSSFHTTKK SGTASTQLGF SDFKDTVELS KKNKRDIVIA FTTHRYKLED IKKQMEYLCL
KNFNPKINII EETESDMVIS VSIKKYYIND KISLYHYLQM YIEYLENNKI IKGYWITMKL
KDNDITVIFG VKIKTPYNIN KIYMIKSIPV SVSKGKISNI NLEIEDVKMY NNNLEEQNGY
RLKFYIDSVT DFINFDTRDV YLELGPWFTY NSFGIQFAEY SIRRRLVSST KEKSMEICYI
ILSKLMCLSS EMYNIKRIRE GKQNVIKSAI HGSSDAITTA AYNNIIDPNN DIYSQILSSQ
IMKLGHGYYD CYLNLNRYDS ININSVTEQD INITSEIIEN F
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