ID NRT24_ARATH Reviewed; 527 AA.
AC Q9FJH8;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 119.
DE RecName: Full=High affinity nitrate transporter 2.4;
DE Short=AtNRT2:4;
GN Name=NRT2.4; OrderedLocusNames=At5g60770; ORFNames=MAE1.2, MAE1.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY.
RX PubMed=11912225; DOI=10.1093/jexbot/53.370.825;
RA Orsel M., Filleur S., Fraisier V., Daniel-Vedele F.;
RT "Nitrate transport in plants: which gene and which control?";
RL J. Exp. Bot. 53:825-833(2002).
RN [5]
RP TISSUE SPECIFICITY, INDUCTION BY NITRATE, AND GENE FAMILY.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [6]
RP FUNCTION, AND INDUCTION BY NITRATE.
RX PubMed=15107992; DOI=10.1007/s00425-004-1266-x;
RA Orsel M., Eulenburg K., Krapp A., Daniel-Vedele F.;
RT "Disruption of the nitrate transporter genes AtNRT2.1 and AtNRT2.2
RT restricts growth at low external nitrate concentration.";
RL Planta 219:714-721(2004).
RN [7]
RP GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
CC -!- FUNCTION: Involved in high-affinity nitrate transport. Might be
CC involved in the transfer of nitrate from stored pools to cytoplasm.
CC {ECO:0000269|PubMed:15107992}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and shoots.
CC {ECO:0000269|PubMed:12668777}.
CC -!- INDUCTION: Low transient up-regulation by nitrate both in shoots and
CC roots. Very low induction by growth on low nitrate concentration, but
CC strong induction in mutant with disruption in both NRT21. and NRT2.2.
CC However, this overexpression could not restore the nitrate influx.
CC {ECO:0000269|PubMed:12668777, ECO:0000269|PubMed:15107992}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; DQ056732; AAY78876.1; -; mRNA.
DR EMBL; AB015472; BAB10098.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97375.1; -; Genomic_DNA.
DR RefSeq; NP_200885.1; NM_125470.2.
DR AlphaFoldDB; Q9FJH8; -.
DR SMR; Q9FJH8; -.
DR BioGRID; 21442; 1.
DR STRING; 3702.Q9FJH8; -.
DR PaxDb; 3702-AT5G60770-1; -.
DR EnsemblPlants; AT5G60770.1; AT5G60770.1; AT5G60770.
DR GeneID; 836198; -.
DR Gramene; AT5G60770.1; AT5G60770.1; AT5G60770.
DR KEGG; ath:AT5G60770; -.
DR Araport; AT5G60770; -.
DR TAIR; AT5G60770; NRT2.4.
DR eggNOG; ENOG502QPIC; Eukaryota.
DR HOGENOM; CLU_024204_0_0_1; -.
DR InParanoid; Q9FJH8; -.
DR OMA; VTWWVMW; -.
DR OrthoDB; 1037860at2759; -.
DR PhylomeDB; Q9FJH8; -.
DR PRO; PR:Q9FJH8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJH8; baseline and differential.
DR Genevisible; Q9FJH8; AT.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0071249; P:cellular response to nitrate; IGI:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:CACAO.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515:SF31; HIGH AFFINITY NITRATE TRANSPORTER 2.4; 1.
DR PANTHER; PTHR23515; HIGH-AFFINITY NITRATE TRANSPORTER 2.3; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Nitrate assimilation; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..527
FT /note="High affinity nitrate transporter 2.4"
FT /id="PRO_0000400101"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 497..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 527 AA; 57768 MW; 9F9BA621E8606AC4 CRC64;
MADGFGEPGS SMHGVTGREQ SYAFSVESPA VPSDSSAKFS LPVDTEHKAK VFKLLSFEAP
HMRTFHLAWI SFFTCFISTF AAAPLVPIIR DNLNLTRQDV GNAGVASVSG SIFSRLVMGA
VCDLLGPRYG CAFLVMLSAP TVFSMSFVGG AGGYITVRFM IGFCLATFVS CQYWMSTMFN
GQIIGLVNGT AAGWGNMGGG VTQLLMPMVY EIIRRLGSTS FTAWRMAFFV PGWMHIIMGI
LVLTLGQDLP DGNRSTLEKK GAVTKDKFSK VLWYAITNYR TWVFVLLYGY SMGVELTTDN
VIAEYFFDRF HLKLHTAGII AASFGMANFF ARPIGGWASD IAARRFGMRG RLWTLWIIQT
LGGFFCLWLG RATTLPTAVV FMILFSLGAQ AACGATFAII PFISRRSLGI ISGLTGAGGN
FGSGLTQLVF FSTSTFSTEQ GLTWMGVMIM ACTLPVTLVH FPQWGSMFLP STEDEVKSTE
EYYYMKEWTE TEKRKGMHEG SLKFAVNSRS ERGRRVASAP SPPPEHV
//
