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Database: UniProt
Entry: Q9FT70
LinkDB: Q9FT70
Original site: Q9FT70 
ID   RQL4B_ARATH             Reviewed;        1150 AA.
AC   Q9FT70; Q9C959;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   16-OCT-2019, entry version 140.
DE   RecName: Full=ATP-dependent DNA helicase Q-like 4B;
DE            EC=3.6.4.12;
DE   AltName: Full=RecQ-like protein 4B;
DE            Short=AtRecQ4B;
DE            Short=AtRecQl4B;
GN   Name=RECQL4B; Synonyms=RECQ4B, RQL4B; OrderedLocusNames=At1g60930;
GN   ORFNames=T7P1.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia; TISSUE=Flower;
RX   PubMed=11058127; DOI=10.1093/nar/28.21.4275;
RA   Hartung F., Plchova H., Puchta H.;
RT   "Molecular characterisation of RecQ homologues in Arabidopsis
RT   thaliana.";
RL   Nucleic Acids Res. 28:4275-4282(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   TISSUE SPECIFICITY, AND INDUCTION BY ABIOTIC STRESSES.
RX   PubMed=12856935; DOI=10.1023/a:1023968429220;
RA   Bagherieh-Najjar M.B., de Vries O.M., Kroon J.T., Wright E.L.,
RA   Elborough K.M., Hille J., Dijkwel P.P.;
RT   "Arabidopsis RecQsim, a plant-specific member of the RecQ helicase
RT   family, can suppress the MMS hypersensitivity of the yeast sgs1
RT   mutant.";
RL   Plant Mol. Biol. 52:273-284(2003).
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=16371241; DOI=10.1016/j.jplph.2005.10.013;
RA   Hartung F., Puchta H.;
RT   "The RecQ gene family in plants.";
RL   J. Plant Physiol. 163:287-296(2006).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=18000056; DOI=10.1073/pnas.0705998104;
RA   Hartung F., Suer S., Puchta H.;
RT   "Two closely related RecQ helicases have antagonistic roles in
RT   homologous recombination and DNA repair in Arabidopsis thaliana.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:18836-18841(2007).
RN   [7]
RP   GENE FAMILY.
RX   PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA   Xu R., Zhang S., Huang J., Zheng C.;
RT   "Genome-wide comparative in silico analysis of the RNA helicase gene
RT   family in Zea mays and Glycine max: a comparison with Arabidopsis and
RT   Oryza sativa.";
RL   PLoS ONE 8:E78982-E78982(2013).
CC   -!- FUNCTION: 3'-5' DNA helicase that may play a role in the repair of
CC       DNA (By similarity). Required to promote but not to suppress
CC       crossovers. {ECO:0000250, ECO:0000269|PubMed:18000056}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Mostly expressed in roots, seedlings, shoots,
CC       shoot apical mersitem, flowers, and siliques.
CC       {ECO:0000269|PubMed:11058127, ECO:0000269|PubMed:12856935}.
CC   -!- INDUCTION: Repressed by cold. {ECO:0000269|PubMed:12856935}.
CC   -!- DISRUPTION PHENOTYPE: Not mutagen-sensitive, and impaired in
CC       hyperrecombination (HR). {ECO:0000269|PubMed:18000056}.
CC   -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG51646.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAG51646.1; Type=Frameshift; Evidence={ECO:0000305};
DR   EMBL; AJ404474; CAC14869.1; -; mRNA.
DR   EMBL; AC018908; AAG51646.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33749.1; -; Genomic_DNA.
DR   PIR; G96634; G96634.
DR   RefSeq; NP_176289.7; NM_104773.7.
DR   SMR; Q9FT70; -.
DR   STRING; 3702.AT1G60930.1; -.
DR   iPTMnet; Q9FT70; -.
DR   PaxDb; Q9FT70; -.
DR   PRIDE; Q9FT70; -.
DR   GeneID; 842384; -.
DR   KEGG; ath:AT1G60930; -.
DR   Araport; AT1G60930; -.
DR   TAIR; locus:2206031; AT1G60930.
DR   eggNOG; KOG0351; Eukaryota.
DR   eggNOG; COG0514; LUCA.
DR   InParanoid; Q9FT70; -.
DR   KO; K10901; -.
DR   OrthoDB; 445763at2759; -.
DR   PhylomeDB; Q9FT70; -.
DR   PRO; PR:Q9FT70; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9FT70; baseline and differential.
DR   Genevisible; Q9FT70; AT.
DR   GO; GO:0005694; C:chromosome; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043138; F:3'-5' DNA helicase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009378; F:four-way junction helicase activity; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0070417; P:cellular response to cold; IEP:UniProtKB.
DR   GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IMP:UniProtKB.
DR   GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR   GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR   InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR010997; HRDC-like_sf.
DR   InterPro; IPR002121; HRDC_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR032284; RecQ_Zn-bd.
DR   InterPro; IPR018982; RQC_domain.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF00570; HRDC; 1.
DR   Pfam; PF16124; RecQ_Zn_bind; 1.
DR   Pfam; PF09382; RQC; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SMART; SM00956; RQC; 1.
DR   SUPFAM; SSF47819; SSF47819; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   TIGRFAMs; TIGR00614; recQ_fam; 1.
DR   PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS50967; HRDC; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN         1   1150       ATP-dependent DNA helicase Q-like 4B.
FT                                /FTId=PRO_0000394530.
FT   DOMAIN      478    653       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      678    823       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   DOMAIN     1029   1111       HRDC. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00328}.
FT   NP_BIND     491    498       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED      327    361       {ECO:0000255}.
FT   MOTIF       597    600       DEAH box.
SQ   SEQUENCE   1150 AA;  128577 MW;  15E3054C24E8D005 CRC64;
     MVVTRGDKFA GSSLACKSMI GANKMSGSHL HEVNNSRSHF PQTNWLKVAK AFECIPSLNK
     FMGSNFLYSL ESQKLGRDRE MAARSIENIA PVTVQTLARP QIEKAWCTLI NLSINNTYLR
     PGITPAIDND STSRTSSTKG STFKVTSNAD GSFCAHNHPE HSQRSVRGTA KSIDSFSSSS
     VGDNKIIIDK VPRVNYEVRD SVTVTNGMEM PPIKNSAQLA RPVEPREVSL GEIDYDDIME
     IIDVDQIAME HCPSTCTKQP SVSKFVDIFT SRREEEQGLP PEICSNCSHG IKLGLCPEAS
     THVEQMKDTL LAISNEILDN TYDLGPDHVE QLHQKRLLLK KQIQQLEILI HNKERKKSQC
     LVSIPSHNTQ YETPQTTNLE VVYGQTDSPT HVKEQGRCVT DNWNMPRDYL VSKERYDISS
     GSEEREQSVS EVIDVTDTES SNDKKWTSSD FPWTKNLEVY NKLVFGNHSF RPNQREIINA
     TMSGCDVFVL MPTGGGKSLT YQLPALLCAG ITLVISPLVS LIQDQIMNLL QTNISAASLS
     AGMEWAEQLE ILQELSSEKS KYKLLYVTPE KVAKSESLLR HLEILNSRSL LARFVIDEAH
     CVSQWGHDFR PDYQGLGVLK QKFPNIPMLA LTATATTSVK EDVVQALGLV NCVVFRQSFN
     RPNLWYSVVP KTNKCLEDID KFIRENHFDE CGIIYCLSRM DCEKVTEALR VFGHKAAFYH
     GSMDPGKRAF VQKQWSKDEI NIICATVAFG MGINKPDVRF VIHHSLPKSI EGYHQECGRA
     GRDGQRSSCV LYYSYTDYIR VKHMISQGGL GQGQMKMGYN CKASSGRMLE TNTENLLRMV
     SYCENEVDCR RFLQLVHLGE KFDSTNCKNT CDNCSSSKIL IDKDVTVIAR QLVALVKLTG
     ERFSSAHIVE IYRGSLNQSV KRNRQDTLHL HGAGKHLTKS EASRILHYLV TEDILAEGVK
     KSELYGSVSS LLKVNRSKAA SLLSGGQSIT MRFPSTIKVS KQSKSTANPA KVPLKQTTLP
     MAKAAPQDSN LSGILLTALK NLRTDIVKES PDLVMAYHIF GNATLKEISK RLPRTKEELL
     DINGLGKAKV SKYGDRLLET IDSTINDHYK TRPGSGKRRR DENVNPNVAE DDDPDWSASQ
     SHKKVVKNKK
//
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