ID CSD_HALSA Reviewed; 415 AA.
AC Q9HMM6;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE RecName: Full=Probable cysteine desulfurase;
DE EC=2.8.1.7;
GN Name=csd; OrderedLocusNames=VNG_2471G;
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX NCBI_TaxID=64091;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-
CC alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA-
CC COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972,
CC ChEBI:CHEBI:64428; EC=2.8.1.7;
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250};
CC -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC aminotransferase family. Csd subfamily. {ECO:0000305}.
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DR EMBL; AE004437; AAG20545.1; -; Genomic_DNA.
DR PIR; E84397; E84397.
DR RefSeq; WP_010903847.1; NC_002607.1.
DR AlphaFoldDB; Q9HMM6; -.
DR SMR; Q9HMM6; -.
DR STRING; 64091.VNG_2471G; -.
DR PaxDb; 64091-VNG_2471G; -.
DR GeneID; 68694982; -.
DR KEGG; hal:VNG_2471G; -.
DR PATRIC; fig|64091.14.peg.1913; -.
DR HOGENOM; CLU_003433_2_5_2; -.
DR InParanoid; Q9HMM6; -.
DR OrthoDB; 5817at2157; -.
DR PhylomeDB; Q9HMM6; -.
DR Proteomes; UP000000554; Chromosome.
DR GO; GO:0031071; F:cysteine desulfurase activity; IEA:UniProtKB-EC.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0006534; P:cysteine metabolic process; IEA:InterPro.
DR CDD; cd06453; SufS_like; 1.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR InterPro; IPR000192; Aminotrans_V_dom.
DR InterPro; IPR010970; Cys_dSase_SufS.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR NCBIfam; TIGR01979; sufS; 1.
DR PANTHER; PTHR43586; CYSTEINE DESULFURASE; 1.
DR PANTHER; PTHR43586:SF8; CYSTEINE DESULFURASE-RELATED; 1.
DR Pfam; PF00266; Aminotran_5; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
PE 3: Inferred from homology;
KW Pyridoxal phosphate; Reference proteome; Transferase.
FT CHAIN 1..415
FT /note="Probable cysteine desulfurase"
FT /id="PRO_0000150326"
FT ACT_SITE 372
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000250"
FT MOD_RES 233
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 415 AA; 45113 MW; 49BAFCA5169B5F37 CRC64;
MEATEQGPID VEAVRADFPI LEREVAGGED LVYLDNAATS HTPEPVVDAI ADYYRRYNSN
VHRGLHELSQ EASVAYEDAH DKLAAFVGGE DREEMVFTKN TTEAENLVAF AWGLNELGPG
DEVVLTQMEH HASLVTWQQV ADETGAEVKY IPITDDGHLD MDAAADMITD DTALVNAVHI
SNTLGTVNPV GELADIAHDH GAYIFVDGAQ AAPTRAVDVQ EIDADFYAFS GHKMLGPTGI
GCLYGKRHLL AEMEPFLYGG DMIERVSYED ATWNDPPWKF EAGTPVIAQG IALAEAVDYL
QDIGMDAIRA HEEALTEYAY DQLTMTDDVD VYGPPGDDRG AVVSFNVDGI HAHDLSSILN
DYGVAIRAGD HCTQPLHDTL GVPASARASF YLYNTRDEVD ALVAAVDEAR QIFAP
//
