ID Q9HSF4_HALSA Unreviewed; 116 AA.
AC Q9HSF4;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 110.
DE SubName: Full=PadR family transcription regulator RosR {ECO:0000313|EMBL:DAC77541.1};
GN Name=rosR {ECO:0000313|EMBL:DAC77541.1};
GN OrderedLocusNames=VNG_0258H {ECO:0000313|EMBL:AAG18853.1};
OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS (Halobacterium halobium).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX NCBI_TaxID=64091 {ECO:0000313|EMBL:AAG18853.1, ECO:0000313|Proteomes:UP000000554};
RN [1] {ECO:0000313|EMBL:AAG18853.1, ECO:0000313|Proteomes:UP000000554}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1
RC {ECO:0000313|Proteomes:UP000000554}, and NRC-1
RC {ECO:0000313|EMBL:AAG18853.1};
RX PubMed=11016950; DOI=10.1073/pnas.190337797;
RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA Spudich J.L., Jung K.W., Alam M., Freitas T., Hou S., Daniels C.J.,
RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA DasSarma S.;
RT "Genome sequence of Halobacterium species NRC-1.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
RN [2] {ECO:0000313|EMBL:DAC77541.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77541.1};
RX PubMed=18313895; DOI=.1016/j.ygeno.2008.01.001;
RA Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT R1 compared to that of strain NRC-1.";
RL Genomics 91:335-346(2008).
RN [3] {ECO:0000313|EMBL:DAC77541.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77541.1};
RX PubMed=26042526;
RA Pfeiffer F., Oesterhelt D.;
RT "A manual curation strategy to improve genome annotation: application to a
RT set of haloarchael genomes.";
RL Life 5:1427-1444(2015).
RN [4] {ECO:0007829|PDB:6EZ1, ECO:0007829|PDB:6F5C}
RP X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
RX PubMed=30110657; DOI=10.1016/j.jsb.2018.08.008;
RA Kutnowski N., Shmuely H., Dahan I., Shmulevich F., Davidov G., Shahar A.,
RA Eichler J., Zarivach R., Shaanan B.;
RT "The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in
RT its ionic shell.";
RL J. Struct. Biol. 204:191-198(2018).
RN [5] {ECO:0000313|EMBL:DAC77541.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77541.1};
RA Pfeiffer F., Marchfelder A., Habermann B.H., Dyall-Smith M.;
RT "The genome of the Halobacterium salinarum type strain is closely related
RT to that of the laboratory strains NRC-1 and R1.";
RL Microbiol. Resour. Announc. 0:0-0(2019).
RN [6] {ECO:0007829|PDB:6QFD, ECO:0007829|PDB:6QH0}
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 6-116 IN COMPLEX WITH MN(2+).
RX PubMed=31310308; DOI=10.1093/nar/gkz604;
RA Kutnowski N., Shmulevich F., Davidov G., Shahar A., Bar-Zvi D., Eichler J.,
RA Zarivach R., Shaanan B.;
RT "Specificity of protein-DNA interactions in hypersaline environment:
RT structural studies on complexes of Halobacterium salinarum oxidative
RT stress-dependent protein hsRosR.";
RL Nucleic Acids Res. 47:8860-8873(2019).
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DR EMBL; AE004437; AAG18853.1; -; Genomic_DNA.
DR EMBL; BK010829; DAC77541.1; -; Genomic_DNA.
DR PIR; A84186; A84186.
DR RefSeq; WP_010902147.1; NC_002607.1.
DR PDB; 6EZ1; X-ray; 1.75 A; A/B=1-116.
DR PDB; 6F5C; X-ray; 1.55 A; A/B=1-116.
DR PDB; 6FAQ; X-ray; 1.95 A; A/B=1-116.
DR PDB; 6FDH; X-ray; 1.85 A; A/B=1-116.
DR PDB; 6QFD; X-ray; 2.13 A; A/B/C/D=6-116.
DR PDB; 6QH0; X-ray; 2.44 A; A/B/C/D=6-116.
DR PDB; 6QIL; X-ray; 2.00 A; A/B/C/D=6-116.
DR PDB; 6QUA; X-ray; 2.68 A; A/B/C/D=6-116.
DR PDBsum; 6EZ1; -.
DR PDBsum; 6F5C; -.
DR PDBsum; 6FAQ; -.
DR PDBsum; 6FDH; -.
DR PDBsum; 6QFD; -.
DR PDBsum; 6QH0; -.
DR PDBsum; 6QIL; -.
DR PDBsum; 6QUA; -.
DR STRING; 64091.VNG_0258H; -.
DR PaxDb; 64091-VNG_0258H; -.
DR GeneID; 68693219; -.
DR KEGG; hal:VNG_0258H; -.
DR PATRIC; fig|64091.14.peg.189; -.
DR HOGENOM; CLU_151709_0_0_2; -.
DR OrthoDB; 180497at2157; -.
DR Proteomes; UP000000554; Chromosome.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR005149; Tscrpt_reg_PadR_N.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF03551; PadR; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6EZ1, ECO:0007829|PDB:6F5C};
KW Metal-binding {ECO:0007829|PDB:6QFD, ECO:0007829|PDB:6QIL};
KW Reference proteome {ECO:0000313|Proteomes:UP000000554}.
FT DOMAIN 22..84
FT /note="Transcription regulator PadR N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03551"
FT BINDING 43
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0007829|PDB:6QIL"
FT BINDING 56
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0007829|PDB:6QIL"
FT BINDING 110
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="3"
FT /evidence="ECO:0007829|PDB:6QIL"
SQ SEQUENCE 116 AA; 13255 MW; D89541CD0BC2A181 CRC64;
MSEAQPDARS DARDLTAFQK NILTVLGEEA RYGLAIKREL EEYYGEEVNH GRLYPNLDDL
VNKGLVEKSE LDKRTNEYAL TNEGFDAVVD DLEWTLSKFV ADADRRERVE TIVADD
//
