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Database: UniProt
Entry: Q9HYT6
LinkDB: Q9HYT6
Original site: Q9HYT6 
ID   RAPA_PSEAE              Reviewed;         950 AA.
AC   Q9HYT6;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   16-OCT-2019, entry version 105.
DE   RecName: Full=RNA polymerase-associated protein RapA {ECO:0000255|HAMAP-Rule:MF_01821};
DE            EC=3.6.4.- {ECO:0000255|HAMAP-Rule:MF_01821};
DE   AltName: Full=ATP-dependent helicase HepA {ECO:0000255|HAMAP-Rule:MF_01821};
GN   Name=rapA {ECO:0000255|HAMAP-Rule:MF_01821}; Synonyms=hepA;
GN   OrderedLocusNames=PA3308;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
OS   JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC   1C / PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Transcription regulator that activates transcription by
CC       stimulating RNA polymerase (RNAP) recycling in case of stress
CC       conditions such as supercoiled DNA or high salt concentrations.
CC       Probably acts by releasing the RNAP, when it is trapped or
CC       immobilized on tightly supercoiled DNA. Does not activate
CC       transcription on linear DNA. Probably not involved in DNA repair.
CC       {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SUBUNIT: Interacts with the RNAP. Has a higher affinity for the
CC       core RNAP than for the holoenzyme. Its ATPase activity is
CC       stimulated by binding to RNAP. {ECO:0000255|HAMAP-Rule:MF_01821}.
CC   -!- SIMILARITY: Belongs to the SNF2/RAD54 helicase family. RapA
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01821}.
DR   EMBL; AE004091; AAG06696.1; -; Genomic_DNA.
DR   PIR; G83231; G83231.
DR   RefSeq; NP_251998.1; NC_002516.2.
DR   RefSeq; WP_003119658.1; NC_002516.2.
DR   SMR; Q9HYT6; -.
DR   PaxDb; Q9HYT6; -.
DR   PRIDE; Q9HYT6; -.
DR   EnsemblBacteria; AAG06696; AAG06696; PA3308.
DR   GeneID; 882473; -.
DR   KEGG; pae:PA3308; -.
DR   PATRIC; fig|208964.12.peg.3464; -.
DR   PseudoCAP; PA3308; -.
DR   eggNOG; ENOG4105BZK; Bacteria.
DR   eggNOG; COG0553; LUCA.
DR   HOGENOM; HOG000218482; -.
DR   InParanoid; Q9HYT6; -.
DR   KO; K03580; -.
DR   OMA; MSILERD; -.
DR   PhylomeDB; Q9HYT6; -.
DR   BioCyc; PAER208964:G1FZ6-3372-MONOMER; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0008094; F:DNA-dependent ATPase activity; IBA:GO_Central.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10810; -; 1.
DR   HAMAP; MF_01821; Helicase_RapA; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR023949; Helicase_RapA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR022737; RapA_C.
DR   InterPro; IPR038718; SNF2-like_sf.
DR   InterPro; IPR000330; SNF2_N.
DR   InterPro; IPR040765; Tudor_1_RapA.
DR   InterPro; IPR040766; Tudor_2_RapA.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF12137; RapA_C; 1.
DR   Pfam; PF00176; SNF2_N; 1.
DR   Pfam; PF18339; Tudor_1_RapA; 1.
DR   Pfam; PF18337; Tudor_RapA; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   Activator; ATP-binding; Complete proteome; DNA-binding; Helicase;
KW   Hydrolase; Nucleotide-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN         1    950       RNA polymerase-associated protein RapA.
FT                                /FTId=PRO_0000207181.
FT   DOMAIN      165    333       Helicase ATP-binding. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   DOMAIN      475    629       Helicase C-terminal. {ECO:0000255|HAMAP-
FT                                Rule:MF_01821}.
FT   NP_BIND     178    185       ATP. {ECO:0000255|HAMAP-Rule:MF_01821}.
FT   MOTIF       279    282       DEAH box.
SQ   SEQUENCE   950 AA;  106872 MW;  26911065FD197FCE CRC64;
     MDMAQYQPGQ RWISDSEAEL GLGTILAQDG RLLTVLYPAT GDTRQYALRN APLTRVRFAP
     GDEVTHFEGW KMTVREVEES EGLLVYHGLT AQNEQRTLPE TQLSNFIQFR LASDRLFAGQ
     IDPLPWFSLR YHTLERRSQL LQSSLWGLGG ARAQPIAHQL HIAREVADRM APRVLLADEV
     GLGKTIEAGL IIHRQLLSGR AGRVLILVPE NLQHQWLVEM RRRFNLQVAL FDKERFAESD
     ASNPFEDTQL ALVALEWLKE DERAQDALFA AGWDLLVVDE AHHLVWHQDQ VSAEYALVEQ
     LAEIIPGVLL LTATPEQLGQ DSHFARLRLL DPNRFHDLEA FRRESEQYRP VAEAVQELLD
     HGSLSAGARK AIHGFLGSEG DELLASVEGG DEEARSRLVR ELLDRHGTGR VLFRNTRAAV
     QGFPQRELHA YPLPMPSQYE ELPAGEHPDL YPEVNFQQQW EDGDDNNRWW RFDPRVEWLI
     DTLKMLKQFK VLVICAHAET ALDLEDALRL RSGISATVFH EGMSILERDR AAAYFADEEF
     GAQVLICSEI GSEGRNFQFA HHLVLFDLPA HPDLLEQRIG RLDRIGQRHT IQLHVPHLEN
     SAQQRLFQWY HQALNAFLNT CPTGNALQHE FGPRLLPLLD GGEDKAWDAL LAEGRARREA
     LEAELHSGRD RLLELNSGGA GEGEALVEAI EEQDDDFALP IYMERLFDAY GIHSEDHSEN
     ALILKPSEKM LDAGFPLGDD EGVTITYDRA QALAREDMQF LTWEHPMVQG GMDLVLSGSM
     GNTSVALIKN KALKPGTVLL ELLFVSEAVA PRALQLGRWL PPQALRCLLD ANGNDLAGRV
     SLETLNDQLE SVPRVSANKF VQAQRDVLAK QFDVAEGKIV PRHEERVARA KQQFAAAMDE
     ELARLTALKA VNPSVRDSEL DSLRTQREEG LALLDKASLR LEAIRILVAG
//
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