ID COBM_PSEAE Reviewed; 250 AA.
AC Q9HZP9;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=Precorrin-4 C(11)-methyltransferase;
DE EC=2.1.1.133;
DE AltName: Full=Precorrin-3 methylase;
GN Name=cobM; OrderedLocusNames=PA2948;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Catalyzes the methylation of C-11 in precorrin-4 to form
CC precorrin-5. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=precorrin-4 + S-adenosyl-L-methionine = precorrin-5 + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:22012, ChEBI:CHEBI:57769,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:77871;
CC EC=2.1.1.133;
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 4/10.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG06336.1; -; Genomic_DNA.
DR PIR; G83276; G83276.
DR RefSeq; NP_251638.1; NC_002516.2.
DR RefSeq; WP_003102926.1; NZ_QZGE01000009.1.
DR AlphaFoldDB; Q9HZP9; -.
DR SMR; Q9HZP9; -.
DR STRING; 208964.PA2948; -.
DR PaxDb; 208964-PA2948; -.
DR GeneID; 882854; -.
DR KEGG; pae:PA2948; -.
DR PATRIC; fig|208964.12.peg.3094; -.
DR PseudoCAP; PA2948; -.
DR HOGENOM; CLU_011276_7_1_6; -.
DR InParanoid; Q9HZP9; -.
DR OrthoDB; 9815856at2; -.
DR PhylomeDB; Q9HZP9; -.
DR BioCyc; PAER208964:G1FZ6-2999-MONOMER; -.
DR UniPathway; UPA00148; UER00215.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0046026; F:precorrin-4 C11-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd11641; Precorrin-4_C11-MT; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR006362; Cbl_synth_CobM/CibF.
DR InterPro; IPR003043; Uropor_MeTrfase_CS.
DR NCBIfam; TIGR01465; cobM_cbiF; 1.
DR PANTHER; PTHR45790:SF4; COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR45790; SIROHEME SYNTHASE-RELATED; 1.
DR Pfam; PF00590; TP_methylase; 1.
DR SUPFAM; SSF53790; Tetrapyrrole methylase; 1.
DR PROSITE; PS00839; SUMT_1; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..250
FT /note="Precorrin-4 C(11)-methyltransferase"
FT /id="PRO_0000287735"
SQ SEQUENCE 250 AA; 27040 MW; 6E40E1D1F6F38FBD CRC64;
MTVYFIGAGP GDPDLITVKG QRLIRQCPVI LYAGSLVPQA LLEGHQAGQV VNTAELDLEQ
IVELLAQAHR RGLDVARVHS GDPSLYGAIG EQIRHLRELG IPYEIVPGVT ATAACAALLG
CELTLPEVSQ TLILTRYAAR TKMPEGESLG DLARHRATLA IHLGVAHLAK IVEELLPHYG
ADCPVAVIHR ASWPDQEQVR GTLGDILPKV AARNFRRTAL ILVGEVLAAE GFADSSLYRA
EHRHLYRPGE
//
