ID Q9I1M5_PSEAE Unreviewed; 333 AA.
AC Q9I1M5;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 116.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN Name=pslN {ECO:0000313|EMBL:AAG05632.1};
GN OrderedLocusNames=PA2244 {ECO:0000313|EMBL:AAG05632.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964 {ECO:0000313|EMBL:AAG05632.1, ECO:0000313|Proteomes:UP000002438};
RN [1] {ECO:0000313|EMBL:AAG05632.1, ECO:0000313|Proteomes:UP000002438}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC 1C / PRS 101 / PAO1 {ECO:0000313|Proteomes:UP000002438};
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R., Smith K.,
RA Spencer D., Wong G.K., Wu Z., Paulsen I.T., Reizer J., Saier M.H.,
RA Hancock R.E., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IB topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00006645}.
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DR EMBL; AE004091; AAG05632.1; -; Genomic_DNA.
DR PIR; H83364; H83364.
DR RefSeq; NP_250934.1; NC_002516.2.
DR RefSeq; WP_003113726.1; NZ_QZGE01000014.1.
DR AlphaFoldDB; Q9I1M5; -.
DR SMR; Q9I1M5; -.
DR STRING; 208964.PA2244; -.
DR PaxDb; 208964-PA2244; -.
DR GeneID; 880773; -.
DR KEGG; pae:PA2244; -.
DR PATRIC; fig|208964.12.peg.2344; -.
DR PseudoCAP; PA2244; -.
DR HOGENOM; CLU_046978_1_1_6; -.
DR InParanoid; Q9I1M5; -.
DR OrthoDB; 9778962at2; -.
DR PhylomeDB; Q9I1M5; -.
DR BioCyc; PAER208964:G1FZ6-2284-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR Gene3D; 1.10.132.120; -; 1.
DR Gene3D; 3.30.66.10; DNA topoisomerase I domain; 1.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR035447; DNA_topo_I_N_sf.
DR InterPro; IPR049331; Top1B_N_bact.
DR InterPro; IPR001631; TopoI.
DR InterPro; IPR014711; TopoI_cat_a-hlx-sub_euk.
DR InterPro; IPR013500; TopoI_cat_euk.
DR Pfam; PF21338; Top1B_N_bact; 1.
DR Pfam; PF01028; Topoisom_I; 1.
DR PRINTS; PR00416; EUTPISMRASEI.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR SUPFAM; SSF55869; DNA topoisomerase I domain; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000002438};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 33..81
FT /note="DNA topoisomerase IB N-terminal"
FT /evidence="ECO:0000259|Pfam:PF21338"
FT DOMAIN 94..267
FT /note="DNA topoisomerase I catalytic core eukaryotic-type"
FT /evidence="ECO:0000259|Pfam:PF01028"
SQ SEQUENCE 333 AA; 38011 MW; C375B47D5238826E CRC64;
MSAAPPDIPA LPAGLCYVDD RQPGIRRRRQ GKRFVYFDAD GQRIGDAEEI RRLDKLAIPP
AYREVWICPD PNGHLQATGR DARGRKQYRY HPRWREVRDA DKYERLLRFG QALPRLRRHI
DAQLRLPGLG RDKVVALAVA LLDATLIRVG NHRYARDNRS YGLTTLRTRH VDVNGSRIRF
RFKGKSGIEH DVSLEHPRLA RVLRRCLELP GQDLLQYLDE DGQPHRIGSH DINEYLRRHT
GEDFSAKDYR TWAGSALALE RLRRAEADGL GAVVAEVAAE LGNSVAICRQ CYIHPAIIEA
YQAGQLGQLR RARKRRWLSA EEATLLAFLD TPG
//
