ID Q9I7P4_DROME Unreviewed; 1615 AA.
AC Q9I7P4;
DT 01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT 30-NOV-2010, sequence version 3.
DT 24-JAN-2024, entry version 147.
DE RecName: Full=chitin synthase {ECO:0000256|ARBA:ARBA00012543};
DE EC=2.4.1.16 {ECO:0000256|ARBA:ARBA00012543};
GN Name=kkv {ECO:0000313|EMBL:AAG22215.3,
GN ECO:0000313|FlyBase:FBgn0001311};
GN Synonyms=Blimp {ECO:0000313|EMBL:AAG22215.3}, blimp
GN {ECO:0000313|EMBL:AAG22215.3}, chitin synthase
GN {ECO:0000313|EMBL:AAG22215.3}, ChSB {ECO:0000313|EMBL:AAG22215.3},
GN CS-1 {ECO:0000313|EMBL:AAG22215.3}, CS-1/kkv
GN {ECO:0000313|EMBL:AAG22215.3}, DmCS-1 {ECO:0000313|EMBL:AAG22215.3},
GN DmeCHS1 {ECO:0000313|EMBL:AAG22215.3}, DmeChSB
GN {ECO:0000313|EMBL:AAG22215.3}, Dmel\CG2666
GN {ECO:0000313|EMBL:AAG22215.3}, Kkv {ECO:0000313|EMBL:AAG22215.3},
GN l(3)82Fh {ECO:0000313|EMBL:AAG22215.3};
GN ORFNames=CG2666 {ECO:0000313|EMBL:AAG22215.3,
GN ECO:0000313|FlyBase:FBgn0001311}, Dmel_CG2666
GN {ECO:0000313|EMBL:AAG22215.3};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [3] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [5] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [6] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [7] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [8] {ECO:0000313|EMBL:AAG22215.3, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-
CC D-glucosamine = [(1->4)-N-acetyl-beta-D-glucosaminyl](n+1) + H(+) +
CC UDP; Xref=Rhea:RHEA:16637, Rhea:RHEA-COMP:9593, Rhea:RHEA-COMP:9595,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17029, ChEBI:CHEBI:57705,
CC ChEBI:CHEBI:58223; EC=2.4.1.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000319};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAG22215.3; -; Genomic_DNA.
DR RefSeq; NP_730928.2; NM_169052.3.
DR CAZy; GT2; Glycosyltransferase Family 2.
DR EnsemblMetazoa; FBtr0301398; FBpp0290640; FBgn0001311.
DR GeneID; 45884; -.
DR UCSC; CG2666-RB; d. melanogaster.
DR AGR; FB:FBgn0001311; -.
DR CTD; 45884; -.
DR FlyBase; FBgn0001311; kkv.
DR VEuPathDB; VectorBase:FBgn0001311; -.
DR GeneTree; ENSGT00530000064569; -.
DR HOGENOM; CLU_004002_0_0_1; -.
DR OrthoDB; 1331060at2759; -.
DR PhylomeDB; Q9I7P4; -.
DR BioGRID-ORCS; 45884; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 45884; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0001311; Expressed in eye disc (Drosophila) and 21 other cell types or tissues.
DR ExpressionAtlas; Q9I7P4; baseline and differential.
DR Genevisible; Q9I7P4; DM.
DR GO; GO:0045177; C:apical part of cell; IDA:FlyBase.
DR GO; GO:0071944; C:cell periphery; IBA:GO_Central.
DR GO; GO:0030428; C:cell septum; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0016020; C:membrane; ISS:FlyBase.
DR GO; GO:0004100; F:chitin synthase activity; ISS:FlyBase.
DR GO; GO:0006038; P:cell wall chitin biosynthetic process; IBA:GO_Central.
DR GO; GO:0006031; P:chitin biosynthetic process; IMP:FlyBase.
DR GO; GO:0008362; P:chitin-based embryonic cuticle biosynthetic process; IMP:FlyBase.
DR GO; GO:0006035; P:cuticle chitin biosynthetic process; IMP:FlyBase.
DR GO; GO:0001838; P:embryonic epithelial tube formation; IMP:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
DR GO; GO:0035158; P:regulation of tube diameter, open tracheal system; IMP:FlyBase.
DR GO; GO:0035159; P:regulation of tube length, open tracheal system; IMP:FlyBase.
DR GO; GO:0009611; P:response to wounding; IEP:FlyBase.
DR GO; GO:0060439; P:trachea morphogenesis; IMP:FlyBase.
DR CDD; cd04190; Chitin_synth_C; 1.
DR InterPro; IPR004835; Chitin_synth.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR22914; CHITIN SYNTHASE; 1.
DR PANTHER; PTHR22914:SF42; CHITIN SYNTHASE; 1.
DR Pfam; PF03142; Chitin_synth_2; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Glycosyltransferase {ECO:0000313|EMBL:AAG22215.3};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000000803};
KW Transferase {ECO:0000313|EMBL:AAG22215.3};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 88..112
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 146..164
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 184..205
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 309..335
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 386..408
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 420..445
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 478..496
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 964..981
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 988..1011
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1017..1038
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1050..1069
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1288..1307
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1345..1366
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 1..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1172..1223
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1578..1615
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1080..1110
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1583..1615
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1615 AA; 182722 MW; 8C14C9FBA87AF107 CRC64;
MSAMRHRPMA PPGQGPGAGT AGEHVDSDDN NFTDDESSPL THDIYGGSQR TIQETKGWDV
FRDPPIKIET GSTANQECLE LTVKILKIFA YVITFIIVLT GGVIAKGTML FMTSQVRKDK
KMEYCNKDLG RDKSFVVRLP EEERVAWIWA LLIAYALPEI GALIRSARIC FFKTFKVPKT
GHFLFVWLME SLSAVGMALL MFVVLPQIDA IQGAMLTNCL CVVPGIFGLL SRTSKEGKRF
VKVIIDLAAV AAQVTGLVIW PLLENRRELW VIPVACVMIS CGWWENYVSP QSPLGLVRAL
GRIKEEMKYT RYFCHIFLSI WKILLFFTVT LLIYWAQGEE PGNLFAMYGD AFGPHKIIVY
ELPAGLGGVL PDTLESANID TVDVDAAYNT VVYVLLLQIF GAYLCYIFGK FACKILIQGF
SYAFPVSLTV PLSVTFLIAA CGIRIDDPCF FHDTIPDYLF FTSPSNFRFN NFVTEQMAWA
WILWLLSQTW IALHIWTPKC ERLATTEKLF VQPMYSSLLI DQSMALNRRR DDQADVKTED
LSEIEKEKGD EYYETISVHT DRSSAPNKPS IKSSDNITRI YSCATMWHET KDEMIEFLKS
IMRMDEDQCA RRVAQKYLRV LDPDYYEFET HIFFDDAFEI SDHSDDDIQC NRFVKLLIAT
MDEAASEIHQ TTIRLRPPKK YPTPYGGRLV WTLPGKTKFI THLKDKDRIR HRKRWSQVMY
MYYLLGHRLM ELPISVDRKD AIAENTYLLT LDGDIDFKPN AVTLLVDLMK KNKNLGAACG
RIHPVGSGPM VWYQLFEYAI GHWLQKATEH MIGCVLCSPG CFSLFRGKAL MDDNVMKKYT
TRSDEARHYV QYDQGEDRWL CTLLLQRGYR VEYSAASDAY THCPEGFNEF YNQRRRWVPS
TIANIMDLLA DAKRTIKIND NISLLYIFYQ MMLMGGTILG PGTIFLMLVG AFVAAFRIDN
WTSFHYNIVP ILAFMFICFT CKSNIQLFVA QVLSTAYALI MMAVIVGTAL QLGEDGIGSP
SAIFLISMVG SFFIAACLHP QEFWCITCGL IYLLSIPSMY LLLILYSIIN LNVVSWGTRE
VVAKKTKKEL EAEKKAAEEA KKRVKQKSML SFLQSGIGDN GDEEGSVEFS LAGLFRCIFC
THGKTSDEKQ QLTSIAESLD TIKHRMDTIE SAVDPHGHHA SRHGRRRTTS SGSKDHHLLT
SVAEKSGDES DESDSDTSAE PKQERDFLTN PYWIEDPDVR KGEVDFLSST EIQFWKDLID
QYLYPIDNDP VEQARIASDL IELRNKSVFA FFMANALFVL IVFLLQLNKD KLHIIWPLGV
KTNITYIEET SEVHISKEYL QLEPIGLVFV FFFALILIIQ FTAMLFHRFG TISHILASTE
LNFCKKKSED LTQDQLIDKH AVEIVKNLQR LQGIDGDYDN DSGSGPDRIA RRKTIQNLEK
ARQPRRQIGT LDVAFKKRFL KLTADAENNP ATPILTRRLT MRAETIRALE VRKNSVMAER
RKSAMQTLGA KNEYGITTGA PINNNGALPN QRSGRVSNAG ISIKDVFNVN GGGAEQIYGS
NGGGTINQGY EHVIDEDGDG NSLRLTTRNP HPHPHHQVSW GQNTNGGGGN GTGRL
//
