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Database: UniProt
Entry: Q9JTJ6
LinkDB: Q9JTJ6
Original site: Q9JTJ6 
ID   GSHB_NEIMA              Reviewed;         318 AA.
AC   Q9JTJ6; A1ISW3;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   13-FEB-2019, entry version 107.
DE   RecName: Full=Glutathione synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            EC=6.3.2.3 {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=GSH synthetase {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSH-S {ECO:0000255|HAMAP-Rule:MF_00162};
DE            Short=GSHase {ECO:0000255|HAMAP-Rule:MF_00162};
DE   AltName: Full=Glutathione synthase {ECO:0000255|HAMAP-Rule:MF_00162};
GN   Name=gshB {ECO:0000255|HAMAP-Rule:MF_00162};
GN   OrderedLocusNames=NMA1747;
OS   Neisseria meningitidis serogroup A / serotype 4A (strain Z2491).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC   Neisseriaceae; Neisseria.
OX   NCBI_TaxID=122587;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Z2491;
RX   PubMed=10761919; DOI=10.1038/35006655;
RA   Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M.,
RA   Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T.,
RA   Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S.,
RA   Jagels K., Leather S., Moule S., Mungall K.L., Quail M.A.,
RA   Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Whitehead S., Spratt B.G., Barrell B.G.;
RT   "Complete DNA sequence of a serogroup A strain of Neisseria
RT   meningitidis Z2491.";
RL   Nature 404:502-506(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + glycine + L-gamma-glutamyl-L-cysteine = ADP +
CC         glutathione + H(+) + phosphate; Xref=Rhea:RHEA:13557,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57305, ChEBI:CHEBI:57925, ChEBI:CHEBI:58173,
CC         ChEBI:CHEBI:456216; EC=6.3.2.3; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00162};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC       Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit. {ECO:0000250};
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_00162}.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00162}.
DR   EMBL; AL157959; CAM08875.1; -; Genomic_DNA.
DR   PIR; C81799; C81799.
DR   ProteinModelPortal; Q9JTJ6; -.
DR   SMR; Q9JTJ6; -.
DR   PRIDE; Q9JTJ6; -.
DR   EnsemblBacteria; CAM08875; CAM08875; NMA1747.
DR   KEGG; nma:NMA1747; -.
DR   HOGENOM; HOG000265022; -.
DR   KO; K01920; -.
DR   OMA; WMRKDPP; -.
DR   UniPathway; UPA00142; UER00210.
DR   Proteomes; UP000000626; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   HAMAP; MF_00162; GSH_S; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP-bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   PANTHER; PTHR21621:SF4; PTHR21621:SF4; 1.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    318       Glutathione synthetase.
FT                                /FTId=PRO_0000197469.
FT   DOMAIN      128    313       ATP-grasp. {ECO:0000255|HAMAP-
FT                                Rule:MF_00162}.
FT   NP_BIND     154    210       ATP. {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       284    284       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
FT   METAL       286    286       Magnesium or manganese.
FT                                {ECO:0000255|HAMAP-Rule:MF_00162}.
SQ   SEQUENCE   318 AA;  35172 MW;  D22681F6229E7BD6 CRC64;
     MKVLFIADPM ASFKTYKDTT YAMMREMAKR GWRLFHTLSG ELSVKNGLVV ADASAFEFLG
     AQDDHDKNWF AAADKVQTAL KAFDAVIMRT DPPFDMQYLY ATQLLTLAEQ QGAKVFNSGQ
     AMRDFNEKLA ILNFSRFTAP TLVTTRSADV RAFLKEHGDI IIKPLDGMGG MGIFRLTEKD
     PNIGSILETL MQLDSRTIMA QRYIPEIVHG DKRILIIGGE VVPYALARIP QNGETRGNLA
     AGGRGVAQEL SERDREIAET LAPELKRRGI LLAGLDVIGS NLTEVNVTSP TGFQEIMKQK
     GFDVAAMFAD AVAEWSVR
//
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