ID Q9K5M2_9NOST Unreviewed; 2258 AA.
AC Q9K5M2;
DT 01-OCT-2000, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 128.
DE SubName: Full=Anabaenopeptilide synthetase ApdA {ECO:0000313|EMBL:AFW94565.1};
DE SubName: Full=Peptide synthetase {ECO:0000313|EMBL:CAC01603.1};
GN Name=adpA {ECO:0000313|EMBL:CAC01603.1};
GN ORFNames=ANA_C11805 {ECO:0000313|EMBL:AFW94565.1};
OS Anabaena sp. 90.
OC Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae; Anabaena.
OX NCBI_TaxID=46234 {ECO:0000313|EMBL:CAC01603.1};
RN [1] {ECO:0000313|EMBL:CAC01603.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=90 {ECO:0000313|EMBL:CAC01603.1};
RX PubMed=10931313; DOI=10.1046/j.1365-2958.2000.01982.x;
RA Rouhiainen L., Paulin L., Suomalainen S., Hyytiainen H., Buikema W.,
RA Haselkorn R., Sivonen K.;
RT "Genes encoding synthetases of cyclic depsipeptides, anabaenopeptilides, in
RT Anabaena strain 90.";
RL Mol. Microbiol. 37:156-167(2000).
RN [2] {ECO:0000313|EMBL:AFW94565.1, ECO:0000313|Proteomes:UP000010101}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=90 {ECO:0000313|EMBL:AFW94565.1};
RX PubMed=23148582; DOI=10.1186/1471-2164-13-613;
RA Wang H., Sivonen K., Rouhiainen L., Fewer D.P., Lyra C., Rantala-Ylinen A.,
RA Vestola J., Jokela J., Rantasarkka K., Li Z., Liu B.;
RT "Genome-derived insights into the biology of the hepatotoxic bloom-forming
RT cyanobacterium Anabaena sp. strain 90.";
RL BMC Genomics 13:613-613(2012).
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR EMBL; CP003284; AFW94565.1; -; Genomic_DNA.
DR EMBL; AJ269505; CAC01603.1; -; Genomic_DNA.
DR STRING; 46234.ANA_C11805; -.
DR KEGG; anb:ANA_C11805; -.
DR PATRIC; fig|46234.3.peg.2014; -.
DR eggNOG; COG1020; Bacteria.
DR HOGENOM; CLU_000022_0_12_3; -.
DR Proteomes; UP000010101; Chromosome chANA01.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd17643; A_NRPS_Cytc1-like; 1.
DR CDD; cd08700; FMT_C_OzmH_like; 1.
DR CDD; cd08649; FMT_core_NRPS_like; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 3.40.50.12230; -; 1.
DR Gene3D; 3.40.50.980; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR011034; Formyl_transferase-like_C_sf.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 3.
DR SUPFAM; SSF50486; FMT C-terminal domain-like; 1.
DR SUPFAM; SSF53328; Formyltransferase; 1.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000010101}.
FT DOMAIN 1087..1162
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2163..2238
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 2258 AA; 253026 MW; 32F5D3AF06D81172 CRC64;
MANHQNKFNC FIIGEGTLPI QCAEILINQG HVIYGIISAD ASIINWAEGK NIPYIKPTDH
LGEFLSQQPF DYLFSIVNRY VLPQEILELP RQFAINYHDA PLPRYAGVNA TSWALMNQEK
THGVTWHIMA AMVDAGDILK QVIIDIADDE TALTLNGKCY ESAINAFAQL VDELSSGTFV
ATKVNLNQRT YFSRFKRLRA GGIISWKRCA YELDALIRAL DFGSYPNPLG RPKLAIDSNL
FIVSKLEVQG NLSNYPPGTI TNIEPTYITV STASYDIALR QVLAINSQAL SISYLVEKFG
LQVGYQFCDL EPNQVKQIEK FDQSIVKHEA FWVERLGILE SITIPEAKQT ASLHLKEPQY
ARARMFVPDE AITLWLQRHP QWHRSDFLAA AFITYLARIG GSGCFDIGFK DIELQRQLVG
LESLFASVVP YRVNIDYEQS FAALKKQFEF TQLPLTYVRD VVTRYPSLRS LSDHRGSEQF
FPVVVERVEK LEDYQSPLGS DLTFIISSDG KKCCWFYNTD VLNDDSIARM QEQFTVFLQG
ILTEPDQCIA YLPLLSEQQR REILLEWNDT QVDDPQDKCI HQLFESQVDL TPDAVAVVFE
NQQLTYHELN CRANQLAHYL QSLGVGTDVL VGICVERSLE MVVGLLGILK AGGAYVPLDP
EYPTERLTFM LADAQVSVLL TQQHLVEKLP ENQEPVVCLD TDWLVICESS QESPITEVQP
GNLAYVIYTS GSTGTPKGVM LSHSNLCNHM SWMQATFPLT EKDKVLQKTP FGFDASVWEF
YAPLLAGGQL LIAKPGGHTD SAYLLRLIAQ QQVTIVQLVP SLLQMLLEQG GIETCHSLKH
VFCGGEVLPV ALLEGLLSKL DVNLHNLYGP TETCIDATFC NCQREIYAQI VPIGRPISNT
QIYILDQNLQ ALPVGVPGEL HISGAGLARG YLNRPELTQE KFIANPFSTY PGSRLYKTGD
LARYLPNGNI EYLGRIDNQV KIRGFRIELA EIEAVLNQRD DVQVACVIAR EDDPGNKRLV
GYIVPSSQMT CTVSELPQFL KTKLPDYMVP NIFVMLDSLP LTPNGKINRG ALPVPDLQGQ
GTDKYVAPRT PTEEILSLIW AQVLKLEQVG IHDNFFTFGG HSLLATQLVS RIRTSFKVEL
PLRSLFAAPT VAQLSPHIQR LQQQELELTS PPILPRAENA ELPLSYAQQR LWFLDQLQPN
SSLYNIPIAL RLVGTLNQAA LAQSLEEIIH RHEALRTNFI TVDGTPRQVI QTATNWTVSV
VNFRHLSTSA QEIAAQQLAQ KQAIQSFDLA SEVLIRVTLV VLSETEHLLN VCMHHIVSDG
WSMGVFVAEL AALYNAYSIG LPSLPDATRI PLTPLPIQYA DFAIWQRNWL VGDVLQSQLN
YWQQQLKDAP ALLVLPTDRP RPAVQTFVGA YQDFALSVEL TQGLMQLSQQ QDCTLFMTLL
AAFDTLLYRY TGTEDILVGT PIANRDRSEI EGLIGFFVNT LVMRSNLSEN PSFAELLTRV
RSMALSAYAH QNLPLEMLVE ALQPERDLSH TPLFQVMFAL QNPYLSEVEL SGLSISSLPI
EGATAKFDLT LAMENTGNGL IGVWEYNTDL FDASTIERMT GHFLTLLSSI VANPLEQISQ
LPLLTEVERQ QLLIEWNHTQ VNYPVDQCIH QLFESQVERT PDAVAVVFEE QQLTYHELNC
RANQLAHYLQ SLGVGADVLV GICVERSWEM IVGLLGILKA GGAYVPLDPE YPPERLSFIL
TDTQVKVLLT QQQLVNKLPA HTAQLVCLDT DLEKITQNSN SNPVNTATSP NLAYVIYTSG
STGQPKGVLV NHHHVTRLFA ATNSWYKFNS QDVWTMFHSY AFDFSVWEIW GALLYGGRLV
VVPYLLTRSP ESFYKLLSQE QVTILNQTPS AFRQLIQAEQ SIGMSNLNLR LVIFGGEALE
LESLQPWFER HGDQKPQLVN MYGITETTVH VTYRPLSLDD LNRTASVIGR PIPDLQVYVL
DQHLQPVPIG VPGEMYVGGE GVTRGYLHRD ELTGQRFISN PFQRSKGGER LYRTGDLARY
LPNGELEYLG RIDQQVKIRG FRIELGEIEG LLAQHPAVWE SVVVVREDEP SNKRLVAYVV
PQVAQAPTTA ELRSFLKKKL PDYMIPNAIV ILESLPLTSN GKIDRRSLPV PESRAGIEES
LVAPRTPVEE KLAQIWAKVL RVEQVGIHDN FFELGGHSLL ATQLVSRIRT SFKVELPLRS
LFAAPTVAEL APLIQQLQQQ NIEIKVPPIL PRKKNAKS
//
