ID VRAB_STAA1 Reviewed; 379 AA.
AC Q9KWK4; A7WZ15;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 27-MAR-2024, entry version 117.
DE RecName: Full=Putative acetyl-CoA C-acetyltransferase VraB;
DE EC=2.3.1.-;
GN Name=vraB; OrderedLocusNames=SAHV_0574;
OS Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=418127;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VANCOMYCIN RESISTANCE.
RX PubMed=10708580; DOI=10.1006/bbrc.2000.2277;
RA Kuroda M., Kuwahara-Arai K., Hiramatsu K.;
RT "Identification of the up- and down-regulated genes in vancomycin-resistant
RT Staphylococcus aureus strains Mu3 and Mu50 by cDNA differential
RT hybridization method.";
RL Biochem. Biophys. Res. Commun. 269:485-490(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mu3 / ATCC 700698;
RX PubMed=17954695; DOI=10.1128/aac.00534-07;
RA Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT "Mutated response regulator graR is responsible for phenotypic conversion
RT of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT resistance to vancomycin-intermediate resistance.";
RL Antimicrob. Agents Chemother. 52:45-53(2008).
CC -!- MISCELLANEOUS: May contribute to vancomycin resistance.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000305}.
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DR EMBL; AB035449; BAB03328.1; -; Genomic_DNA.
DR EMBL; AP009324; BAF77457.1; -; Genomic_DNA.
DR PIR; B89826; B89826.
DR RefSeq; WP_001070676.1; NC_009782.1.
DR AlphaFoldDB; Q9KWK4; -.
DR SMR; Q9KWK4; -.
DR KEGG; saw:SAHV_0574; -.
DR HOGENOM; CLU_031026_2_1_9; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:InterPro.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR43853; 3-KETOACYL-COA THIOLASE, PEROXISOMAL; 1.
DR PANTHER; PTHR43853:SF3; ACETYL-COA C-ACETYLTRANSFERASE YHFS-RELATED; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00737; THIOLASE_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Transferase.
FT CHAIN 1..379
FT /note="Putative acetyl-CoA C-acetyltransferase VraB"
FT /id="PRO_0000206425"
FT ACT_SITE 86
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000250"
FT ACT_SITE 338
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 379 AA; 41094 MW; B62F36E0A437A771 CRC64;
MNQAVIVAAK RTAFGKYGGT LKHLEPEQLL KPLFQHFKEK YPEVISKIDD VVLGNVVGNG
GNIARKALLE AGLKDSIPGV TIDRQCGSGL ESVQYACRMI QAGAGKVYIA GGVESTSRAP
WKIKRPHSVY ETALPEFYER ASFAPEMSDP SMIQGAENVA KMYDVSRELQ DEFAYRSHQL
TAENVKNGNI SQEILPITVK GEIFNTDESL KSHIPKDNFG RFKPVIKGGT VTAANSCMKN
DGAVLLLIME KDMAYELGFE HGLLFKDGVT VGVDSNFPGI GPVPAISNLL KRNQLTIENI
EVIEINEAFS AQVVACQQAL NISNTQLNIW GGALASGHPY GASGAQLVTR LFYMFDKETM
IASMGIGGGL GNAALFTRF
//
