ID TRXM3_ARATH Reviewed; 173 AA.
AC Q9SEU7; Q42072; Q67XN4; Q67ZH7; Q681Y9; Q9SKS6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 27-MAR-2024, entry version 164.
DE RecName: Full=Thioredoxin M3, chloroplastic;
DE Short=AtTrxm3;
DE AltName: Full=Protein GFP ARRESTED TRAFFICKING 1;
DE Flags: Precursor;
GN Name=GAT1; OrderedLocusNames=At2g15570; ORFNames=F9O13.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10580150; DOI=10.1016/s0378-1119(99)00448-5;
RA Mestres-Ortega D., Meyer Y.;
RT "The Arabidopsis thaliana genome encodes at least four thioredoxins m and a
RT new prokaryotic-like thioredoxin.";
RL Gene 240:307-316(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 142-173.
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19825616; DOI=10.1093/mp/ssn076;
RA Chibani K., Wingsle G., Jacquot J.P., Gelhaye E., Rouhier N.;
RT "Comparative genomic study of the thioredoxin family in photosynthetic
RT organisms with emphasis on Populus trichocarpa.";
RL Mol. Plant 2:308-322(2009).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=19259774; DOI=10.1007/s11103-009-9471-4;
RA Cain P., Hall M., Schroder W.P., Kieselbach T., Robinson C.;
RT "A novel extended family of stromal thioredoxins.";
RL Plant Mol. Biol. 70:273-281(2009).
RN [9]
RP FUNCTION.
RX PubMed=19820302;
RA Benitez-Alfonso Y., Jackson D.;
RT "Redox homeostasis regulates plasmodesmal communication in Arabidopsis
RT meristems.";
RL Plant Signal. Behav. 4:655-659(2009).
RN [10]
RP FUNCTION, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19218459; DOI=10.1073/pnas.0808717106;
RA Benitez-Alfonso Y., Cilia M., San Roman A., Thomas C., Maule A., Hearn S.,
RA Jackson D.;
RT "Control of Arabidopsis meristem development by thioredoxin-dependent
RT regulation of intercellular transport.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:3615-3620(2009).
CC -!- FUNCTION: Thiol-disulfide oxidoreductase required for maintaining
CC symplastic permeability in the meristem. Involved in redox regulation
CC of callose deposition, plasmodesmata cell-to-cell communication and
CC meristem maintenance. {ECO:0000269|PubMed:19218459,
CC ECO:0000269|PubMed:19820302}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:19218459, ECO:0000269|PubMed:19259774}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9SEU7-1; Sequence=Displayed;
CC -!- DEVELOPMENTAL STAGE: Expressed during embryogenesis in the root
CC meristem at the transition from heart to torpedo stage. At the
CC cotyledon stage, expressed in root and shoot meristems, with weak
CC expression in provascular tissues. Expressed later in the seedling root
CC meristem, shoot apex, and vasculature. During flowering, expressed in
CC inflorescence and floral meristems, and in petal, stamen, and carpel
CC primordia. {ECO:0000269|PubMed:19218459}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethality when homozygous. Increased
CC callose deposition and accumulation of reactive oxygen species in
CC roots. {ECO:0000269|PubMed:19218459}.
CC -!- SIMILARITY: Belongs to the thioredoxin family. Plant M-type subfamily.
CC {ECO:0000305}.
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DR EMBL; AF095751; AAF15950.1; -; mRNA.
DR EMBL; AC006248; AAD17401.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06417.1; -; Genomic_DNA.
DR EMBL; AK175478; BAD43241.1; -; mRNA.
DR EMBL; AK176140; BAD43903.1; -; mRNA.
DR EMBL; AK176784; BAD44547.1; -; mRNA.
DR EMBL; BT025735; ABF83625.1; -; mRNA.
DR EMBL; Z26209; CAA81191.1; -; mRNA.
DR PIR; F84530; F84530.
DR RefSeq; NP_179159.1; NM_127118.4. [Q9SEU7-1]
DR AlphaFoldDB; Q9SEU7; -.
DR SMR; Q9SEU7; -.
DR STRING; 3702.Q9SEU7; -.
DR PaxDb; 3702-AT2G15570-2; -.
DR ProteomicsDB; 232396; -. [Q9SEU7-1]
DR EnsemblPlants; AT2G15570.1; AT2G15570.1; AT2G15570. [Q9SEU7-1]
DR GeneID; 816050; -.
DR Gramene; AT2G15570.1; AT2G15570.1; AT2G15570. [Q9SEU7-1]
DR KEGG; ath:AT2G15570; -.
DR Araport; AT2G15570; -.
DR TAIR; AT2G15570; ATHM3.
DR eggNOG; KOG0910; Eukaryota.
DR HOGENOM; CLU_090389_0_2_1; -.
DR InParanoid; Q9SEU7; -.
DR OMA; AASTFHC; -.
DR PhylomeDB; Q9SEU7; -.
DR PRO; PR:Q9SEU7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SEU7; baseline and differential.
DR Genevisible; Q9SEU7; AT.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0009570; C:chloroplast stroma; TAS:UniProtKB.
DR GO; GO:0045454; P:cell redox homeostasis; IMP:UniProtKB.
DR GO; GO:0010497; P:plasmodesmata-mediated intercellular transport; IMP:UniProtKB.
DR GO; GO:0010647; P:positive regulation of cell communication; IMP:UniProtKB.
DR GO; GO:0048509; P:regulation of meristem development; IMP:UniProtKB.
DR CDD; cd02947; TRX_family; 1.
DR Gene3D; 3.40.30.10; Glutaredoxin; 1.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR InterPro; IPR017937; Thioredoxin_CS.
DR InterPro; IPR013766; Thioredoxin_domain.
DR PANTHER; PTHR45663; GEO12009P1; 1.
DR PANTHER; PTHR45663:SF21; THIOREDOXIN M3, CHLOROPLASTIC; 1.
DR Pfam; PF00085; Thioredoxin; 1.
DR PRINTS; PR00421; THIOREDOXIN.
DR SUPFAM; SSF52833; Thioredoxin-like; 1.
DR PROSITE; PS00194; THIOREDOXIN_1; 1.
DR PROSITE; PS51352; THIOREDOXIN_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Disulfide bond; Electron transport;
KW Plastid; Redox-active center; Reference proteome; Transit peptide;
KW Transport.
FT TRANSIT 1..67
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 68..173
FT /note="Thioredoxin M3, chloroplastic"
FT /id="PRO_0000034164"
FT DOMAIN 68..173
FT /note="Thioredoxin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT ACT_SITE 96
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 99
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT SITE 97
FT /note="Contributes to redox potential value"
FT /evidence="ECO:0000250"
FT SITE 98
FT /note="Contributes to redox potential value"
FT /evidence="ECO:0000250"
FT DISULFID 96..99
FT /note="Redox-active"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00691"
FT CONFLICT 110
FT /note="A -> T (in Ref. 4; BAD43241)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="A -> E (in Ref. 1; AAF15950)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="I -> V (in Ref. 4; BAD44547)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 173 AA; 19500 MW; F103CA26FCB87613 CRC64;
MAISSSSSSI CFNPTRFHTA RHISSPSRLF PVTSFSPRSL RFSDRRSLLS SSASRLRLSP
LCVRDSRAAE VTQRSWEDSV LKSETPVLVE FYTSWCGPCR MVHRIIDEIA GDYAGKLNCY
LLNADNDLPV AEEYEIKAVP VVLLFKNGEK RESIMGTMPK EFYISAIERV LNS
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