ID Q9TVK6_DICDI Unreviewed; 1342 AA.
AC Q9TVK6; Q55BN1;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 121.
DE SubName: Full=NPC1 protein {ECO:0000313|EMBL:AAF25229.1};
DE SubName: Full=Niemann-Pick C type protein {ECO:0000313|EMBL:EAL71929.1};
GN Name=npcA {ECO:0000313|EMBL:EAL71929.1};
GN ORFNames=DDB_G0269158 {ECO:0000313|EMBL:EAL71929.1};
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689 {ECO:0000313|EMBL:AAF25229.1};
RN [1] {ECO:0000313|EMBL:AAF25229.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Ax3 {ECO:0000313|EMBL:AAF25229.1};
RA Xu J., Postlethwait P., Steck T.L.;
RT "NPC1,A gene related to cholesterol homeostasis in Dictyostelium
RT Discoideum.";
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EAL71929.1, ECO:0000313|Proteomes:UP000002195}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000313|EMBL:EAL71929.1,
RC ECO:0000313|Proteomes:UP000002195};
RX PubMed=15875012; DOI=10.1038/nature03481;
RG The Dictyostelium discoideum Sequencing Consortium;
RA Eichinger L., Pachebat J.A., Glockner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M., Spiegler S., Tivey A., Sugano S.,
RA White B., Walker D., Woodward J., Winckler T., Tanaka Y., Shaulsky G.,
RA Schleicher M., Weinstock G., Rosenthal A., Cox E.C., Chisholm R.L.,
RA Gibbs R., Loomis W.F., Platzer M., Kay R.R., Williams J., Dear P.H.,
RA Noegel A.A., Barrell B., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3] {ECO:0000313|EMBL:EAL71929.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=AX4 {ECO:0000313|EMBL:EAL71929.1};
RG The Dictyostelium discoideum Sequencing Consortium;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.-A., Sucgang R.,
RA Song J., Cox E.C., Tunggal B., Szafranski K., Konfortov B.A.,
RA Farbrother P., Bankier A.T., Lehmann R., Hamlin N., Xu Q., Davies R.,
RA Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.,
RA Davis P., Nie X., Kerhornou A., Hemphill L., Bason N., Berriman M.,
RA Desany B., Churcher C., Cooper J., van Driessche N., Cronin A.,
RA Goodhead I., Muzny D., Hall N., Harper D., Lindsay R., Hauser H., James K.,
RA Quiles M., Buchrieser C., Wardroper A., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K., Price C., Ma J., Quail M.,
RA Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Weinstock G.,
RA Sharp S., Just E., Shaulsky G., Simmonds M., Tivey A., White B., Walker D.,
RA Woodward J., Winckler T., Schleicher M., Rosenthal A., Rivero F.,
RA Chisholm R.L., Gibbs R., Loomis W.F., Platzer M., Kay R.R., Williams J.,
RA Dear P.H., Noegel A.A., Barrell B., Kuspa A.;
RL Submitted (AUG-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the patched family.
CC {ECO:0000256|ARBA:ARBA00005585}.
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DR EMBL; AF202725; AAF25228.1; -; mRNA.
DR EMBL; AF202726; AAF25229.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71929.1; -; Genomic_DNA.
DR RefSeq; XP_646800.1; XM_641708.1.
DR AlphaFoldDB; Q9TVK6; -.
DR SMR; Q9TVK6; -.
DR STRING; 44689.Q9TVK6; -.
DR TCDB; 2.A.6.6.7; the resistance-nodulation-cell division (rnd) superfamily.
DR PaxDb; 44689-DDB0191127; -.
DR GeneID; 8617773; -.
DR KEGG; ddi:DDB_G0269158; -.
DR dictyBase; DDB_G0269158; npcA.
DR eggNOG; KOG1933; Eukaryota.
DR HOGENOM; CLU_002359_0_1_1; -.
DR OMA; WWFDVES; -.
DR Reactome; R-DDI-8963678; Intestinal lipid absorption.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005319; F:lipid transporter activity; IEA:InterPro.
DR GO; GO:0032934; F:sterol binding; IBA:GO_Central.
DR GO; GO:0015918; P:sterol transport; IBA:GO_Central.
DR Gene3D; 1.20.1640.10; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR InterPro; IPR004765; NPC1-like.
DR InterPro; IPR032190; NPC1_N.
DR InterPro; IPR003392; Ptc/Disp.
DR InterPro; IPR000731; SSD.
DR NCBIfam; TIGR00917; 2A060601; 1.
DR PANTHER; PTHR45727; NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1; 1.
DR PANTHER; PTHR45727:SF10; NPC1 PROTEIN; 1.
DR Pfam; PF16414; NPC1_N; 1.
DR Pfam; PF02460; Patched; 1.
DR SUPFAM; SSF82866; Multidrug efflux transporter AcrB transmembrane domain; 2.
DR PROSITE; PS50156; SSD; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000002195};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..1342
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5011429368"
FT TRANSMEM 252..276
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 288..307
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 332..358
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 424..442
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 668..688
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 700..727
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 739..762
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 816..841
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 927..945
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1165..1185
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1192..1212
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1224..1245
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1266..1284
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1296..1319
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 663..841
FT /note="SSD"
FT /evidence="ECO:0000259|PROSITE:PS50156"
SQ SEQUENCE 1342 AA; 148023 MW; CC24655B1A3ACEC3 CRC64;
MNINQIMSLF VCFCLFSSIY LINTVHSQGP FPYNTTEGCS MNGLNASYIE SGLYPPYHNQ
ILAPGTCQYS HPEYSETSCC TNGQTLVLLS NMVMGSAVFA RCPACLFNVW DLWCASSCSP
YQQSFMVPTS IDSKTGQILS VDFVIHPDYA WGLYNSCSGV NSDGAPPFGT IFHTPQDFFS
GIFALNPAFS INWVFNETGY ISPTAPCAES CSCDQCGDAC GPPPTVGEIG LFNNSLPQTY
LFNHEIPFLS VWMIWTFIMF TLVLTYGTSM GLVLFGNVKF FDSTKVKVIA SLVLSISFIS
AILIPFISGK VPLHDSSCQW KMPSDQLWNC SLALGVGIYE LITFIFLFVI SIVIIVLYHI
GSNNRPIPGS SSNASVVYDA LISSNSSNND NSNSNNNEQQ QQQPSTIIQR FFYWYGHKVA
SKPLIVILIC IIITAGLGIG IIKLEIEQDP VKLWVAPDSR AALDKTYFDN NFGPFYRVEQ
IIITPKNQTK YPTVIEYDLL LELFEIELSL MSLEAEYEGE LITLDSLCFK PTKRGCLIES
TSGLWQRNIS KLNYTQSEEG VLTYYENCQS SLLQPDCMDS VGAPVQPRVV LGGWENNNSS
AASAFVVTFL LNNPTDMVNT AMAWEQVWLD HISAIAATSE IFDITYSAER SVQDELSREG
NADIPTILIS YFVMFFYVSL SLGSYYPFPT RFLSLFVRSR FALGFCGIII VAASIVISVG
VCSMANLKAT LIISEVIPFL VLAIGVDNIF IIVNTFESLH VVRYDPHTRA AILPTSEDSL
ARTLAKVGPS IALASLSESL AFLLGSLTKM PAVQAFSYYA ALAIFVDFLL QVSAFSALLV
LDSKRASSRR IDCLPCIALE DGDNSDMEDD DDEENEKLPF ARADINANFK NSQPTYSTSS
SPKKTTLLQV VFKKFYAPFL LHPITKMLVI IFFVGLLLTG INFAFQVSIG LDQRVALPSN
SYLQGYFNNM ANLLEVGPPF YIVIKGDYNY TDFESQNKLC TMGGCDKDSI VNVFNNAPFI
NKGISSWLDD YISFAQAQGS CCLKYPNGSI CYNGADPSCA PCFSNNAQGR PDPQSFIQYL
PFFLNVSNTA SCPLAGLAYT SDAHIVNGTI VASRFDGYHT TLRTQDDYIN AVQTAYYLAD
NSDLDVEVYS IIYVYFDQYL TIKSVAIMDI LLALGGVFIV CLILLLNPLV SFLVVISVGM
ICVDLLGIMA LWNISLNAVS VVNVVMAIGI GIEFCVHIAS TFINAPKHFS RDQKAKYAVT
EMGSSIISGI FITKLLGVVV LGFSTSEIFT VYYFRMYLSI VFLGGLHGLV FLPVLLSLFG
TDTLQLDRLC KSKKNSYSLN DE
//
