ID RN150_HUMAN Reviewed; 438 AA.
AC Q9ULK6; Q3T1D0; Q6ZNW6;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 2.
DT 27-MAR-2024, entry version 153.
DE RecName: Full=RING finger protein 150;
DE Flags: Precursor;
GN Name=RNF150; Synonyms=KIAA1214;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=10574462; DOI=10.1093/dnares/6.5.337;
RA Nagase T., Ishikawa K., Kikuno R., Hirosawa M., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XV. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 6:337-345(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Salivary gland;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 92-438 (ISOFORM 3).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- INTERACTION:
CC Q9ULK6-3; Q92993: KAT5; NbExp=3; IntAct=EBI-36513929, EBI-399080;
CC Q9ULK6-3; Q8TAP4-4: LMO3; NbExp=3; IntAct=EBI-36513929, EBI-11742507;
CC Q9ULK6-3; P17252: PRKCA; NbExp=3; IntAct=EBI-36513929, EBI-1383528;
CC Q9ULK6-3; Q15047-2: SETDB1; NbExp=3; IntAct=EBI-36513929, EBI-9090795;
CC Q9ULK6-3; Q5BVD1: TTMP; NbExp=3; IntAct=EBI-36513929, EBI-10243654;
CC Q9ULK6-3; P61981: YWHAG; NbExp=3; IntAct=EBI-36513929, EBI-359832;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9ULK6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ULK6-2; Sequence=VSP_023845;
CC Name=3;
CC IsoId=Q9ULK6-3; Sequence=VSP_023846, VSP_023847;
CC Name=4;
CC IsoId=Q9ULK6-4; Sequence=VSP_023843, VSP_023844;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA86528.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB033040; BAA86528.1; ALT_INIT; mRNA.
DR EMBL; AK130520; BAC85369.1; -; mRNA.
DR EMBL; AC093905; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC096733; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC097475; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC101992; AAI01993.1; -; mRNA.
DR CCDS; CCDS34065.1; -. [Q9ULK6-1]
DR RefSeq; NP_065775.1; NM_020724.1. [Q9ULK6-1]
DR RefSeq; XP_011530450.1; XM_011532148.2. [Q9ULK6-4]
DR AlphaFoldDB; Q9ULK6; -.
DR SMR; Q9ULK6; -.
DR BioGRID; 121553; 16.
DR IntAct; Q9ULK6; 17.
DR STRING; 9606.ENSP00000425840; -.
DR GlyCosmos; Q9ULK6; 4 sites, No reported glycans.
DR GlyGen; Q9ULK6; 4 sites.
DR iPTMnet; Q9ULK6; -.
DR PhosphoSitePlus; Q9ULK6; -.
DR BioMuta; RNF150; -.
DR DMDM; 134035025; -.
DR MassIVE; Q9ULK6; -.
DR MaxQB; Q9ULK6; -.
DR PaxDb; 9606-ENSP00000425840; -.
DR PeptideAtlas; Q9ULK6; -.
DR ProteomicsDB; 85063; -. [Q9ULK6-1]
DR ProteomicsDB; 85064; -. [Q9ULK6-2]
DR ProteomicsDB; 85065; -. [Q9ULK6-3]
DR ProteomicsDB; 85066; -. [Q9ULK6-4]
DR Antibodypedia; 27282; 175 antibodies from 22 providers.
DR DNASU; 57484; -.
DR Ensembl; ENST00000306799.7; ENSP00000304321.3; ENSG00000170153.11. [Q9ULK6-2]
DR Ensembl; ENST00000420921.6; ENSP00000394581.2; ENSG00000170153.11. [Q9ULK6-4]
DR Ensembl; ENST00000507500.5; ENSP00000425568.1; ENSG00000170153.11. [Q9ULK6-3]
DR Ensembl; ENST00000515673.7; ENSP00000425840.1; ENSG00000170153.11. [Q9ULK6-1]
DR GeneID; 57484; -.
DR KEGG; hsa:57484; -.
DR MANE-Select; ENST00000515673.7; ENSP00000425840.1; NM_020724.2; NP_065775.1.
DR UCSC; uc003iio.2; human. [Q9ULK6-1]
DR AGR; HGNC:23138; -.
DR CTD; 57484; -.
DR DisGeNET; 57484; -.
DR GeneCards; RNF150; -.
DR HGNC; HGNC:23138; RNF150.
DR HPA; ENSG00000170153; Low tissue specificity.
DR neXtProt; NX_Q9ULK6; -.
DR OpenTargets; ENSG00000170153; -.
DR PharmGKB; PA134918555; -.
DR VEuPathDB; HostDB:ENSG00000170153; -.
DR eggNOG; KOG0800; Eukaryota.
DR GeneTree; ENSGT00940000156171; -.
DR HOGENOM; CLU_049885_2_1_1; -.
DR InParanoid; Q9ULK6; -.
DR OMA; ECDATKS; -.
DR OrthoDB; 5474929at2759; -.
DR PhylomeDB; Q9ULK6; -.
DR TreeFam; TF317486; -.
DR PathwayCommons; Q9ULK6; -.
DR SignaLink; Q9ULK6; -.
DR SIGNOR; Q9ULK6; -.
DR BioGRID-ORCS; 57484; 12 hits in 1186 CRISPR screens.
DR ChiTaRS; RNF150; human.
DR GenomeRNAi; 57484; -.
DR Pharos; Q9ULK6; Tdark.
DR PRO; PR:Q9ULK6; -.
DR Proteomes; UP000005640; Chromosome 4.
DR RNAct; Q9ULK6; Protein.
DR Bgee; ENSG00000170153; Expressed in left ventricle myocardium and 181 other cell types or tissues.
DR ExpressionAtlas; Q9ULK6; baseline and differential.
DR Genevisible; Q9ULK6; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd02122; PA_GRAIL_like; 1.
DR CDD; cd16805; RING-H2_RNF150; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR46539; E3 UBIQUITIN-PROTEIN LIGASE ATL42; 1.
DR PANTHER; PTHR46539:SF25; RING FINGER PROTEIN 150; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Membrane; Metal-binding;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix; Zinc;
KW Zinc-finger.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..438
FT /note="RING finger protein 150"
FT /id="PRO_0000280697"
FT TOPO_DOM 35..208
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..438
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 81..183
FT /note="PA"
FT ZN_FING 278..319
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 186
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..141
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_023843"
FT VAR_SEQ 142..161
FT /note="AVVIFNVGSNTNETITMPHA -> MSSHAQPLKLISPCPYSLSS (in
FT isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_023844"
FT VAR_SEQ 204..245
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10574462"
FT /id="VSP_023845"
FT VAR_SEQ 402..405
FT /note="QEPA -> FCHT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023846"
FT VAR_SEQ 406..438
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023847"
SQ SEQUENCE 438 AA; 48072 MW; 12E495CD0793DF85 CRC64;
MAMSLIQACC SLALSTWLLS FCFVHLLCLD FTVAEKEEWY TAFVNITYAE PAPDPGAGAA
GGGGAELHTE KTECGRYGEH SPKQDARGEV VMASSAHDRL ACDPNTKFAA PTRGKNWIAL
IPKGNCTYRD KIRNAFLQNA SAVVIFNVGS NTNETITMPH AGVEDIVAIM IPEPKGKEIV
SLLERNITVT MYITIGTRNL QKYVSRTSVV FVSISFIVLM IISLAWLVFY YIQRFRYANA
RDRNQRRLGD AAKKAISKLQ IRTIKKGDKE TESDFDNCAV CIEGYKPNDV VRILPCRHLF
HKSCVDPWLL DHRTCPMCKM NILKALGIPP NADCMDDLPT DFEGSLGGPP TNQITGASDT
TVNESSVTLD PAVRTVGALQ VVQDTDPIPQ EGDVIFTTNS EQEPAVSSDS DISLIMAMEV
GLSDVELSTD QDCEEVKS
//
