ID Q9VJW1_DROME Unreviewed; 1653 AA.
AC Q9VJW1; Q9NKE8;
DT 01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2002, sequence version 2.
DT 27-MAR-2024, entry version 181.
DE SubName: Full=LD29171p {ECO:0000313|EMBL:AAM52683.1};
DE SubName: Full=Secretory 71 {ECO:0000313|EMBL:AAF53331.2};
GN Name=Sec71 {ECO:0000313|EMBL:AAF53331.2,
GN ECO:0000313|FlyBase:FBgn0028538};
GN Synonyms=BG:DS00797.7 {ECO:0000313|EMBL:AAF53331.2}, BIG1
GN {ECO:0000313|EMBL:AAF53331.2}, Dmel\CG7578
GN {ECO:0000313|EMBL:AAF53331.2}, sec71 {ECO:0000313|EMBL:AAF53331.2};
GN ORFNames=CG7578 {ECO:0000313|EMBL:AAF53331.2,
GN ECO:0000313|FlyBase:FBgn0028538}, Dmel_CG7578
GN {ECO:0000313|EMBL:AAF53331.2};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [3] {ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [5] {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [6] {ECO:0000313|EMBL:AAM52683.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Berkeley {ECO:0000313|EMBL:AAM52683.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [8] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RA Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S.,
RA Svirskas R., Rubin G.;
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [9] {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [10] {ECO:0000313|EMBL:AAF53331.2, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
RN [11] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109357; DOI=.1534/g3.115.018929;
RG FlyBase Consortium;
RA Matthews B.B., Dos Santos G., Crosby M.A., Emmert D.B., St Pierre S.E.,
RA Gramates L.S., Zhou P., Schroeder A.J., Falls K., Strelets V., Russo S.M.,
RA Gelbart W.M., null;
RT "Gene Model Annotations for Drosophila melanogaster: Impact of High-
RT Throughput Data.";
RL G3 (Bethesda) 5:1721-1736(2015).
RN [12] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=26109356; DOI=.1534/g3.115.018937;
RG FlyBase Consortium;
RA Crosby M.A., Gramates L.S., Dos Santos G., Matthews B.B., St Pierre S.E.,
RA Zhou P., Schroeder A.J., Falls K., Emmert D.B., Russo S.M., Gelbart W.M.,
RA null;
RT "Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.";
RL G3 (Bethesda) 5:1737-1749(2015).
RN [13] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=25589440;
RA Hoskins R.A., Carlson J.W., Wan K.H., Park S., Mendez I., Galle S.E.,
RA Booth B.W., Pfeiffer B.D., George R.A., Svirskas R., Krzywinski M.,
RA Schein J., Accardo M.C., Damia E., Messina G., Mendez-Lago M.,
RA de Pablos B., Demakova O.V., Andreyeva E.N., Boldyreva L.V., Marra M.,
RA Carvalho A.B., Dimitri P., Villasante A., Zhimulev I.F., Rubin G.M.,
RA Karpen G.H., Celniker S.E.;
RT "The Release 6 reference sequence of the Drosophila melanogaster genome.";
RL Genome Res. 25:445-458(2015).
RN [14] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RG Berkeley Drosophila Genome Project;
RA Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R.,
RA Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C.,
RA Rubin G.;
RT "Drosophila melanogaster release 4 sequence.";
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
RN [15] {ECO:0000313|EMBL:AAF53331.2}
RP NUCLEOTIDE SEQUENCE.
RG FlyBase;
RL Submitted (NOV-2022) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; AE014134; AAF53331.2; -; Genomic_DNA.
DR EMBL; AY122171; AAM52683.1; -; mRNA.
DR RefSeq; NP_609675.2; NM_135831.3.
DR SMR; Q9VJW1; -.
DR STRING; 7227.FBpp0080114; -.
DR PaxDb; 7227-FBpp0080114; -.
DR EnsemblMetazoa; FBtr0080537; FBpp0080114; FBgn0028538.
DR GeneID; 34785; -.
DR KEGG; dme:Dmel_CG7578; -.
DR UCSC; CG7578-RA; d. melanogaster.
DR AGR; FB:FBgn0028538; -.
DR CTD; 34785; -.
DR FlyBase; FBgn0028538; Sec71.
DR VEuPathDB; VectorBase:FBgn0028538; -.
DR eggNOG; KOG0929; Eukaryota.
DR GeneTree; ENSGT00940000168468; -.
DR HOGENOM; CLU_000691_1_2_1; -.
DR OMA; MENQVYE; -.
DR OrthoDB; 204547at2759; -.
DR BioGRID-ORCS; 34785; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34785; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0028538; Expressed in thoracico-abdominal ganglion (Drosophila) and 24 other cell types or tissues.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0005802; C:trans-Golgi network; IDA:FlyBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:FlyBase.
DR GO; GO:0031267; F:small GTPase binding; IPI:FlyBase.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IGI:FlyBase.
DR GO; GO:0045807; P:positive regulation of endocytosis; IMP:FlyBase.
DR GO; GO:1904801; P:positive regulation of neuron remodeling; IMP:FlyBase.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IC:FlyBase.
DR CDD; cd00171; Sec7; 1.
DR Gene3D; 1.10.220.20; -; 1.
DR Gene3D; 1.10.1000.11; Arf Nucleotide-binding Site Opener,domain 2; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR032629; DCB_dom.
DR InterPro; IPR015403; Mon2/Sec7/BIG1-like_HDS.
DR InterPro; IPR032691; Mon2/Sec7/BIG1-like_HUS.
DR InterPro; IPR046455; Sec7/BIG1-like_C.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR PANTHER; PTHR10663; GUANYL-NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR10663:SF375; LD29171P; 1.
DR Pfam; PF20252; BIG2_C; 1.
DR Pfam; PF16213; DCB; 1.
DR Pfam; PF01369; Sec7; 1.
DR Pfam; PF09324; Sec7-like_HDS; 1.
DR Pfam; PF12783; Sec7-like_HUS; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF48425; Sec7 domain; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 1: Evidence at protein level;
KW Protein transport {ECO:0000256|ARBA:ARBA00022927};
KW Proteomics identification {ECO:0007829|PeptideAtlas:Q9VJW1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000803};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 575..765
FT /note="SEC7"
FT /evidence="ECO:0000259|PROSITE:PS50190"
FT REGION 221..243
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..566
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 535..566
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1653 AA; 187813 MW; 21697AACBBC342F5 CRC64;
MHNNSTKTKE MFIVRALEKI LADKDIRRSH HSQLKKSCDS ALEQIKAELI SAGQIAEGNE
LPCAALPLPK NDAASIINAE TYFLPFELAC KSRSPRIVVT ALDCLQKLIA YGHLTGSIQD
SANPGHLLID RIVVTIYGCF SGPQTDEAVQ LQIIKALLTV VTSQHVEIHE FTLLQAVRTC
YDIYLSSKNL VNQTTARATL TQMLNVIFAR MENQVYELPP PNSNPTNGSI HSEDCNGSGE
ESLRDSDEVI ASELLAEIIS AAYNEAMKDQ ESVGEPEPTL NGNDYSSHSD HDSVELHSEN
DAVVTAKFTH ILQKDAFLVF RALCKLSMKP LPDGHPDPKS HELRSKVLSL HLLLLILQNA
GPVFRSNEMF IMAIKQYLCV ALSNNGVSLV PEVFELSLSI FVALLSNFKV HLKRQIEVFF
KEIFLNILEA NSSSFEHKWM VIQALTRICA DAQSVVDIYV NYDCDFSAAN LFERLVNDLS
KIAQGRQALE LGANPMQEKS MRIRGLECLV SILKCMVEWS KDLYVNPNMP VPPMQVQSPT
STEQDQADTT IQTMHSGSSH SLNSNQEQLQ DLPEALEERK MRKEVMETGI ELFNRKPQKG
VQFLQEKQLL GATCGDIARW LHEDERLDKT VIGNYIGEND DHSKEVMCAY IDAFDFRQME
VVAALRFLLE GFRLPGEAQK IDRLMEKFAS RYCECNPKNQ LFQSADTVYV LAFSIIMLTT
DLHSPQVKHK MTKEQYIKMN RGISDSKSDL PEEYLSSIYD EISEHEIKMK NNSGMLQQAK
PTGKQAFITE KRRKLLWNME MEVISLTATN LMQSVSHVKS PFTSAKHLEH VRPMFKMAWT
PFLAAFSVGL QDCDDPEIAT LCLDGIRCAI RIACIFHMSL ERDAYVQALA RFTLLNANSP
INEMKAKNID TIKTLIMVAH TDGNYLGSSW LDIVKCISQL ELAQLIGTGV RPQFLSGAQT
TLKDSLNPSV KEHIGETSSQ SVVVAVDRIF TGSMRLDGDA IVDFVKALCQ VSVDELQQQQ
PRMFSLQKIV EISYYNMERI RLQWSRIWQV LGEHFNAVGC NSNEEISFFA LDSLRQLSMK
FMEKGEFSNF RFQKDFLRPF EHIMKKNASP AIRDMVVRCI AQMVNSQAHN IRSGWKNIFS
IFHLAAGDNE EPIVELAFQT TGKIIGDLYK RQFAIMVDSF QDAVKCLSEF ATARFPDTSM
EAIRLVRTCA QCVHEAPQLF AEHAGMENDA SVAEEDRVWV RGWFPMLFSL SCVVNRCKLD
VRTRALTVLF EIVKTYGESF KPHWWKDLFN VIFRIFDNMK LPEHVTEKSE WMTTTCNHAL
YAIIDVFTQY FDVLGHLLLE ELFAQLHWCV QQSNEQLARS GTNCLENLVI SNGFKFNEST
WDKTCQCILD IFNATLPQDL LSWRPKAHSS NNIPQEHNHF EALHIRCVVQ LELIQTMDNI
VFFPATSRKE DAETLAQAAA DLTGGRSGSQ SQLLECQREE QGMYGYLRTR QLLTLADCLM
QSHRFAKRFN ADHDQRSLLW RAGFKGSVKP NLLKQETSSL ACVLRIFFKM YGDENRRSDW
PGIEQELVQV CKEALGYYLS LQSEAHRDAW TSLLLLILTR LLKMSDARFA THVSNYYSLL
CEMMCFDLKP ELRSVLRRVF MRIGPVFNIM SVK
//
