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Database: UniProt
Entry: Q9W5E0
LinkDB: Q9W5E0
Original site: Q9W5E0 
ID   SUV42_DROME             Reviewed;        1300 AA.
AC   Q9W5E0; O77261; Q8T029; Q8T3U6;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   16-JAN-2019, entry version 133.
DE   RecName: Full=Histone-lysine N-methyltransferase Suv4-20;
DE            EC=2.1.1.43;
DE   AltName: Full=Suppressor of variegation 4-20;
DE            Short=Su(var)4-20;
GN   Name=Hmt4-20; Synonyms=Suv4-20; ORFNames=CG13363;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta;
OC   Pterygota; Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Oregon-R;
RX   PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA   Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D.,
RA   Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E.,
RA   Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D.,
RA   Minana B., Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S.,
RA   Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B.,
RA   Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F.,
RA   Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A.,
RA   Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA   McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA   Glover D.M.;
RT   "From sequence to chromosome: the tip of the X chromosome of D.
RT   melanogaster.";
RL   Science 287:2220-2222(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE
RP   SEQUENCE [LARGE SCALE MRNA] OF 453-1300 (ISOFORM 1).
RC   STRAIN=Berkeley; TISSUE=Embryo, and Ovary;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=15145825; DOI=10.1101/gad.300704;
RA   Schotta G., Lachner M., Sarma K., Ebert A., Sengupta R., Reuter G.,
RA   Reinberg D., Jenuwein T.;
RT   "A silencing pathway to induce H3-K9 and H4-K20 trimethylation at
RT   constitutive heterochromatin.";
RL   Genes Dev. 18:1251-1262(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-831; SER-833 AND
RP   THR-930, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
CC   -!- FUNCTION: Histone methyltransferase that specifically
CC       trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation
CC       represents a specific tag for epigenetic transcriptional
CC       repression. Mainly functions in pericentric heterochromatin
CC       regions, thereby playing a central role in the establishment of
CC       constitutive heterochromatin in these regions. Acts as a dominant
CC       suppressor of position-effect variegation.
CC       {ECO:0000269|PubMed:15145825}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-lysyl-[histone] + S-adenosyl-L-methionine = H(+) +
CC         N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:10024, Rhea:RHEA-COMP:9845, Rhea:RHEA-COMP:9846,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:61929; EC=2.1.1.43;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00903};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:15145825}.
CC       Chromosome {ECO:0000305|PubMed:15145825}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9W5E0-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9W5E0-2; Sequence=VSP_024060, VSP_024061;
CC         Note=No experimental confirmation available.;
CC   -!- SIMILARITY: Belongs to the class V-like SAM-binding
CC       methyltransferase superfamily. Histone-lysine methyltransferase
CC       family. Suvar4-20 subfamily. {ECO:0000255|PROSITE-
CC       ProRule:PRU00903}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL90255.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
CC       Sequence=CAA20894.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AE014298; AAF45537.1; -; Genomic_DNA.
DR   EMBL; AL031581; CAA20894.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY069604; AAL39749.1; -; mRNA.
DR   EMBL; AY089517; AAL90255.1; ALT_INIT; mRNA.
DR   PIR; T13389; T13389.
DR   RefSeq; NP_001245453.1; NM_001258524.1. [Q9W5E0-1]
DR   RefSeq; NP_569853.1; NM_130497.4. [Q9W5E0-1]
DR   UniGene; Dm.4968; -.
DR   ProteinModelPortal; Q9W5E0; -.
DR   SMR; Q9W5E0; -.
DR   BioGrid; 57583; 10.
DR   IntAct; Q9W5E0; 3.
DR   STRING; 7227.FBpp0070146; -.
DR   iPTMnet; Q9W5E0; -.
DR   PaxDb; Q9W5E0; -.
DR   PRIDE; Q9W5E0; -.
DR   EnsemblMetazoa; FBtr0070151; FBpp0070146; FBgn0025639. [Q9W5E0-1]
DR   EnsemblMetazoa; FBtr0308652; FBpp0300875; FBgn0025639. [Q9W5E0-1]
DR   GeneID; 31015; -.
DR   KEGG; dme:Dmel_CG13363; -.
DR   UCSC; CG13363-RA; d. melanogaster. [Q9W5E0-1]
DR   CTD; 31015; -.
DR   FlyBase; FBgn0025639; Hmt4-20.
DR   eggNOG; KOG2589; Eukaryota.
DR   eggNOG; ENOG410XPH8; LUCA.
DR   GeneTree; ENSGT00940000173121; -.
DR   InParanoid; Q9W5E0; -.
DR   KO; K11429; -.
DR   OMA; ISSTKRW; -.
DR   PhylomeDB; Q9W5E0; -.
DR   Reactome; R-DME-3214841; PKMTs methylate histone lysines.
DR   ChiTaRS; Hmt4-20; fly.
DR   GenomeRNAi; 31015; -.
DR   PRO; PR:Q9W5E0; -.
DR   Proteomes; UP000000803; Chromosome X.
DR   Bgee; FBgn0025639; Expressed in 27 organ(s), highest expression level in cleaving embryo.
DR   ExpressionAtlas; Q9W5E0; baseline and differential.
DR   Genevisible; Q9W5E0; DM.
DR   GO; GO:0005720; C:nuclear heterochromatin; IDA:FlyBase.
DR   GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR   GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR   GO; GO:0042799; F:histone methyltransferase activity (H4-K20 specific); IDA:FlyBase.
DR   GO; GO:0006342; P:chromatin silencing; IMP:FlyBase.
DR   GO; GO:0034772; P:histone H4-K20 dimethylation; IMP:FlyBase.
DR   GO; GO:0034770; P:histone H4-K20 methylation; IMP:FlyBase.
DR   GO; GO:0034773; P:histone H4-K20 trimethylation; IMP:FlyBase.
DR   GO; GO:0016571; P:histone methylation; IDA:FlyBase.
DR   GO; GO:0040029; P:regulation of gene expression, epigenetic; IMP:UniProtKB.
DR   InterPro; IPR025790; Hist-Lys_N-MTase_Suvar4-20.
DR   InterPro; IPR001214; SET_dom.
DR   Pfam; PF00856; SET; 1.
DR   SMART; SM00317; SET; 1.
DR   PROSITE; PS51570; SAM_MT43_SUVAR420_2; 1.
DR   PROSITE; PS50280; SET; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chromatin regulator; Chromosome;
KW   Complete proteome; Methyltransferase; Nucleus; Phosphoprotein;
KW   Reference proteome; Repressor; S-adenosyl-L-methionine; Transcription;
KW   Transcription regulation; Transferase.
FT   CHAIN         1   1300       Histone-lysine N-methyltransferase Suv4-
FT                                20.
FT                                /FTId=PRO_0000281797.
FT   DOMAIN      255    366       SET. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00190}.
FT   COMPBIAS     20    153       Ser-rich.
FT   COMPBIAS    537    559       His-rich.
FT   COMPBIAS    765    820       Ser-rich.
FT   MOD_RES     831    831       Phosphoserine.
FT                                {ECO:0000269|PubMed:18327897}.
FT   MOD_RES     833    833       Phosphoserine.
FT                                {ECO:0000269|PubMed:18327897}.
FT   MOD_RES     930    930       Phosphothreonine.
FT                                {ECO:0000269|PubMed:18327897}.
FT   VAR_SEQ     764    782       NSSSSSNKATTITNCNNHN -> IAVDHHNSSTRMCINIFA
FT                                T (in isoform 2).
FT                                {ECO:0000303|PubMed:12537569}.
FT                                /FTId=VSP_024060.
FT   VAR_SEQ     783   1300       Missing (in isoform 2).
FT                                {ECO:0000303|PubMed:12537569}.
FT                                /FTId=VSP_024061.
SQ   SEQUENCE   1300 AA;  137525 MW;  7186D0198CC16926 CRC64;
     MVVGSNHTRR GETGSRFTNS SSSSSTSGGP TASASSTTSV TSSLATNSTS TSTAAALLSS
     MSHKSHGPPP SAPPSAHHQT NQQHHQVAHS QPHATHYQQT NQHPSHHHQS HQSSNGSGGG
     SAGSGSGSGS VVSGLNGCNG SAVSRLSQST GMSPRELSEN DDLATSLILD PHLGFQTHKM
     NIRFRPLKVD TQQLKAIVDD FIHTQNYDIA IQRIYEGPWI PRHLKNKNKI ATKRLHDHIV
     RYLRVFDKDS GFAIEACYRY TLEEQRGAKI SSTKRWSKND KIECLVGCIA ELTEAEEAAL
     LHSGKNDFSV MYSCRKNCAQ LWLGPAAYIN HDCRANCKFL ATGRDTACVK VLRDIEVGEE
     ITCFYGEDFF GDSNRYCECE TCERRGTGAF AGKDDGLMLG LSMGLGLASS GPGNNGGYRL
     RETDNRINRI KSRANSTNST SNSNSNTNDS TGPSETSSTN GLVASGGAGG ATGAAMLPTP
     SQQSTGGKEA TAAVSLLEKK LPNVVVSPLT MKELRQKGMT KYDAEMIMAN AAYQQQHHHQ
     HHFHHHHHHH HHHHNHGQHA STGAEATAAV QQMAAMQKPG VGGTGAAGNA GATTVSSVAA
     GAGSEVNGGR STSLRKSMRV NSTSSSISTA SADEVIAPVV AASISLPSKA PVVLMPRCKP
     AQMAIAALHQ SQQRQLRRSE RQKEKLTDGE SSDTSSEQQK KEQKQQDHQL PQKMFSLAEE
     PQPEKSEEKQ QEQQKRVTRN SAGRVGLVAR LATAHNNNIA TTTNSSSSSN KATTITNCNN
     HNSNNSSRIN HNSNLSSRLS VKSRKPAPSE ASSIPSSTSS ENQQQQATRR SCSPTPAYKK
     NLLASFDPDP PSTQGIKEQL KDESVTYSPV KQKRSRRAAA LAAAQSIHCE ALGGFPTGST
     GSQRKRAQAG EPTTSCSSTT ISNVEPLLKT PERRLKLTLR MKRSPILDEV IELGTSLSNG
     GAGRGAPGSH REGTAGEGSV RSALNLTGSS SNGIEYEILR MEGISEHGND DDEDEEEDDE
     EPAAEEEEEP PPKEELQLVN KKQRKKQRSR SRSSQRRSPA PSSVYGTPQK KRLRLIFGNE
     SHTIDIPPAA AEGSGSGLDD LNSSGGGGDE SFNASYASST SLTVNTSSSS TSSSSGVGGA
     TSTSAEPVDS STVGPPIAAQ SPSSTTSSSF QSACTSTTNS NSYFPNGKQR AAGEDSYAMH
     YYQLGKFAGT PSPGQGQAIV SSSSGSSGGG GSGAGFLSMP KHTFGTCALL APTSFACLQN
     QPQISQQKTS SGGGAGVVPT STSTGAVTSH HHTNNHHGQK
//
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