ID Q9X2D4_THEMA Unreviewed; 250 AA.
AC Q9X2D4; G4FGK1;
DT 01-NOV-1999, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1999, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=CRISPR-associated endoribonuclease {ECO:0000256|PIRNR:PIRNR005054};
GN OrderedLocusNames=TM_1814 {ECO:0000313|EMBL:AAD36877.1};
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogota; Thermotogae; Thermotogales; Thermotogaceae;
OC Thermotoga.
OX NCBI_TaxID=243274 {ECO:0000313|EMBL:AAD36877.1, ECO:0000313|Proteomes:UP000008183};
RN [1] {ECO:0000313|EMBL:AAD36877.1, ECO:0000313|Proteomes:UP000008183}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8
RC {ECO:0000313|Proteomes:UP000008183};
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D., Heidelberg J.,
RA Sutton G.G., Fleischmann R.D., White O., Salzberg S.L., Smith H.O.,
RA Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC {ECO:0000256|PIRNR:PIRNR005054}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000256|ARBA:ARBA00005937, ECO:0000256|PIRNR:PIRNR005054}.
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DR EMBL; AE000512; AAD36877.1; -; Genomic_DNA.
DR PIR; C72209; C72209.
DR RefSeq; NP_229611.1; NC_000853.1.
DR RefSeq; WP_004082360.1; NZ_CP011107.1.
DR AlphaFoldDB; Q9X2D4; -.
DR PaxDb; 243274-THEMA_05125; -.
DR EnsemblBacteria; AAD36877; AAD36877; TM_1814.
DR KEGG; tma:TM1814; -.
DR KEGG; tmw:THMA_1859; -.
DR PATRIC; fig|243274.17.peg.1824; -.
DR InParanoid; Q9X2D4; -.
DR OrthoDB; 9797488at2; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd21140; Cas6_I-like; 1.
DR Gene3D; 3.30.70.1890; -; 1.
DR Gene3D; 3.30.70.1900; -; 1.
DR InterPro; IPR049435; Cas_Cas6_C.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR NCBIfam; TIGR01877; cas_cas6; 1.
DR PANTHER; PTHR36984; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR PANTHER; PTHR36984:SF1; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR Pfam; PF21350; Cas6_I-A; 1.
DR Pfam; PF01881; Cas_Cas6_C; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Reference proteome {ECO:0000313|Proteomes:UP000008183};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 124..244
FT /note="CRISPR associated protein Cas6 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01881"
FT ACT_SITE 27
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT ACT_SITE 42
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT SITE 51
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-1"
SQ SEQUENCE 250 AA; 29612 MW; 98AB9975FCA853E9 CRC64;
MRLKVSFQAM ESTVPLNYNY FLSSFIYKRL ASQNENFARF LHEKGYGKRF KFFTFSQLFF
ENSRVSGERI FIFPGKGWWY ISSPVVEFVR YMFSSLSEDP VIRVGKTEFI VKSIDIENSL
PDQSEYHFVM LSPLVVSVPE ENNGKLYHRY LHPGEEEFYE VFRKNLMKKY RAFYGKDPEG
TVEVIPDWDY IKSRHRITKR IKLKNAFVRA VVFPFKIRGE KKLVEIGYEA GFGEKNSMGF
GMVALKKYER
//
