ID Q9XXN0_CAEEL Unreviewed; 415 AA.
AC Q9XXN0;
DT 01-NOV-1999, integrated into UniProtKB/TrEMBL.
DT 01-NOV-1999, sequence version 1.
DT 27-MAR-2024, entry version 153.
DE SubName: Full=Pseudouridine synthase I TruA alpha/beta domain-containing protein {ECO:0000313|EMBL:CAA16518.1};
GN Name=pus-1 {ECO:0000313|EMBL:CAA16518.1,
GN ECO:0000313|WormBase:W06H3.2a};
GN ORFNames=CELE_W06H3.2 {ECO:0000313|EMBL:CAA16518.1}, W06H3.2
GN {ECO:0000313|WormBase:W06H3.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000313|EMBL:CAA16518.1, ECO:0000313|Proteomes:UP000001940};
RN [1] {ECO:0000313|EMBL:CAA16518.1, ECO:0000313|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000313|EMBL:CAA16518.1,
RC ECO:0000313|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RA Sulson J.E., Waterston R.;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; BX284605; CAA16518.1; -; Genomic_DNA.
DR PIR; T26253; T26253.
DR RefSeq; NP_507242.1; NM_074841.6.
DR AlphaFoldDB; Q9XXN0; -.
DR SMR; Q9XXN0; -.
DR STRING; 6239.W06H3.2a.1; -.
DR EPD; Q9XXN0; -.
DR PaxDb; 6239-W06H3-2a; -.
DR PeptideAtlas; Q9XXN0; -.
DR EnsemblMetazoa; W06H3.2a.1; W06H3.2a.1; WBGene00004248.
DR GeneID; 180123; -.
DR KEGG; cel:CELE_W06H3.2; -.
DR UCSC; W06H3.2a; c. elegans.
DR AGR; WB:WBGene00004248; -.
DR WormBase; W06H3.2a; CE20158; WBGene00004248; pus-1.
DR eggNOG; KOG2553; Eukaryota.
DR GeneTree; ENSGT00950000183160; -.
DR HOGENOM; CLU_021971_3_0_1; -.
DR InParanoid; Q9XXN0; -.
DR OMA; FTEDNVQ; -.
DR OrthoDB; 12038at2759; -.
DR PhylomeDB; Q9XXN0; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00004248; Expressed in embryo and 4 other cell types or tissues.
DR ExpressionAtlas; Q9XXN0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 1: Evidence at protein level;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Proteomics identification {ECO:0007829|EPD:Q9XXN0,
KW ECO:0007829|PeptideAtlas:Q9XXN0};
KW Reference proteome {ECO:0000313|Proteomes:UP000001940};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 188..297
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 380..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 92
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 152
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 415 AA; 47221 MW; 55F10E779DE097F2 CRC64;
MTKTVEEQTV DKLNHVQSGG VHKEPKKPRV KQAKYAMLLG YQGKKYYGMQ LQKDLPTIES
KLLEAMETAG WITTAQKEKP FDFFFQRAAR TDRAVSAARQ MCGMQLPRDD AKYQAEGAGI
LNNILPADIR VFGMRRTTNF FHPQKQCDHR TYSYTCPSFV FAKPTELTNS SFRLSPETLA
EVNSILSIYL GTHNFFNYTA KRAYDDMSSN RYIISFECKE PFLFRDDFRK EDVEFVQIVI
KGQSFVLHQI RKMVGMVITV IRELHLKSSI QRSFEGQRMD IPMAPGLGLL LERTHYDSYD
RKNSKSHEPL TDWGEIIEKE IERTKFELIT KDMIETELLT QGMLKWLADL VHHDFTANAE
AEEPEKKTFV TMAAACASKA AKEQDGVAPE AQDAPKEAEA PAEVTPEPVA ATSSG
//
