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Database: UniProt
Entry: Q9ZRZ8
LinkDB: Q9ZRZ8
Original site: Q9ZRZ8 
ID   RH28_ARATH              Reviewed;         789 AA.
AC   Q9ZRZ8; O23506; Q0WRF7;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   16-OCT-2019, entry version 128.
DE   RecName: Full=DEAD-box ATP-dependent RNA helicase 28;
DE            EC=3.6.4.13;
GN   Name=RH28; OrderedLocusNames=At4g16630; ORFNames=dl4340c, FCAALL.424;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae;
OC   Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9862990; DOI=10.1093/nar/27.2.628;
RA   Aubourg S., Kreis M., Lecharny A.;
RT   "The DEAD box RNA helicase family in Arabidopsis thaliana.";
RL   Nucleic Acids Res. 27:628-636(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9461215; DOI=10.1038/35140;
RA   Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA   Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L.,
RA   Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P.,
RA   Wedler H., Wedler E., Wambutt R., Weitzenegger T., Pohl T., Terryn N.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A.,
RA   Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S.,
RA   Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B.,
RA   Mueller-Auer S., Silvey M., James R., Monfort A., Pons A.,
RA   Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P.,
RA   Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T.,
RA   Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W.,
RA   Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W.,
RA   Klosterman S., Schueller C., Chalwatzis N.;
RT   "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of
RT   Arabidopsis thaliana.";
RL   Nature 391:485-488(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana
RT   reference genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17168887; DOI=10.1111/j.1467-7652.2004.00084.x;
RA   Mingam A., Toffano-Nioche C., Brunaud V., Boudet N., Kreis M.,
RA   Lecharny A.;
RT   "DEAD-box RNA helicases in Arabidopsis thaliana: establishing a link
RT   between quantitative expression, gene structure and evolution of a
RT   family of genes.";
RL   Plant Biotechnol. J. 2:401-415(2004).
RN   [7]
RP   GENE FAMILY.
RX   PubMed=24265739; DOI=10.1371/journal.pone.0078982;
RA   Xu R., Zhang S., Huang J., Zheng C.;
RT   "Genome-wide comparative in silico analysis of the RNA helicase gene
RT   family in Zea mays and Glycine max: a comparison with Arabidopsis and
RT   Oryza sativa.";
RL   PLoS ONE 8:E78982-E78982(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC   -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD
CC       box family of RNA helicases and controls ATP binding and
CC       hydrolysis.
CC   -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX27/DRS1
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB10438.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB78705.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
DR   EMBL; AJ010475; CAA09214.1; -; mRNA.
DR   EMBL; Z97341; CAB10438.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161544; CAB78705.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE83778.1; -; Genomic_DNA.
DR   EMBL; AK228353; BAF00292.1; -; mRNA.
DR   PIR; D71433; D71433.
DR   PIR; T51310; T51310.
DR   RefSeq; NP_193396.3; NM_117764.5.
DR   SMR; Q9ZRZ8; -.
DR   BioGrid; 12657; 4.
DR   IntAct; Q9ZRZ8; 4.
DR   STRING; 3702.AT4G16630.1; -.
DR   iPTMnet; Q9ZRZ8; -.
DR   PaxDb; Q9ZRZ8; -.
DR   PRIDE; Q9ZRZ8; -.
DR   EnsemblPlants; AT4G16630.1; AT4G16630.1; AT4G16630.
DR   GeneID; 827364; -.
DR   Gramene; AT4G16630.1; AT4G16630.1; AT4G16630.
DR   KEGG; ath:AT4G16630; -.
DR   Araport; AT4G16630; -.
DR   TAIR; locus:2130918; AT4G16630.
DR   eggNOG; KOG0338; Eukaryota.
DR   eggNOG; COG0513; LUCA.
DR   HOGENOM; HOG000265456; -.
DR   InParanoid; Q9ZRZ8; -.
DR   KO; K13181; -.
DR   OMA; AEVTINH; -.
DR   OrthoDB; 268859at2759; -.
DR   PhylomeDB; Q9ZRZ8; -.
DR   PRO; PR:Q9ZRZ8; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9ZRZ8; baseline and differential.
DR   Genevisible; Q9ZRZ8; AT.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR   InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR   Pfam; PF00270; DEAD; 1.
DR   Pfam; PF00271; Helicase_C; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
DR   PROSITE; PS51195; Q_MOTIF; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Complete proteome; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome; RNA-binding.
FT   CHAIN         1    789       DEAD-box ATP-dependent RNA helicase 28.
FT                                /FTId=PRO_0000239168.
FT   DOMAIN      198    372       Helicase ATP-binding.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00541}.
FT   DOMAIN      402    546       Helicase C-terminal.
FT                                {ECO:0000255|PROSITE-ProRule:PRU00542}.
FT   NP_BIND     211    218       ATP. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00541}.
FT   COILED       13     66       {ECO:0000255}.
FT   COILED      110    135       {ECO:0000255}.
FT   COILED      563    591       {ECO:0000255}.
FT   COILED      628    677       {ECO:0000255}.
FT   MOTIF       167    195       Q motif.
FT   MOTIF       320    323       DEAD box.
FT   COMPBIAS     14    148       Glu-rich.
SQ   SEQUENCE   789 AA;  89355 MW;  DF1CCAB61A4DD66D CRC64;
     MPSSFFFEDA SDDELELIRN QEDSSEEDVK EGEAEEHEAG EDEDGEEEYE EEDDDEEEED
     EKRKRDADAQ SPWDFASYSS SVGEEHARRH TTSIDEKISK AIQHRPVPIS INEEEEEEEE
     EEDASDAETD KQEEYLSEDD EAAEYKPEDA TPKPFFSTVD GVSFHADTFM ELNLSRPLLR
     ACETLGYKKP TPIQAACIPL ALTGRDLCAS AITGSGKTAA FALPTLERLL FRPKRVFATR
     VLILTPTREL AVQIHSMIQN LAQFTDIKCG LIVGGLSVRE QEVVLRSMPD IVVATPGRMI
     DHLRNSMSVD LDDLAVLILD EADRLLQTGF ATEITELVRL CPKRRQTMLF SATMTEEVKE
     LVKLSLNKPL RLSADPSARR PPGLTEEVVR IRRTREANQE AVLLSLCTRT FKSKVIIFSG
     TKQAAHRLKI LFGLAGLKAA ELHGNLTQAQ RLDSLELFRK QEVDFLIATD VAARGLDIIG
     VQTVINYACP REIDSYVHRV GRTARAGREG YAVTFVTDSD RSLLKVIAKK VGSKLKSRVI
     PEQSIVKWSQ IIDEMEDQYS AVISAERDER ALRKAEMEFA KAENMLEHRD EIYARPKRTW
     FMTEKEKKLV AQAEKDSAGN PAGGELVSAD RAEDLKMKEK RKREREKNLP RKKRRKLEAA
     REMLEDNEGE EEEEDEEGDE KRGRSRGKDK KKQETDKKGL TLKDLGYMRA KAVKAKQRAI
     DSGKMERPKP DKKQSRSKPR NQPRGEEMKD LFKSDMGEKK QGRGGAAAAA KPRTKSKNSF
     KSKARYKRR
//
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