ID QSEG_ECO57 Reviewed; 237 AA.
AC P0AD45; P37328; P76997;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 22-NOV-2005, sequence version 1.
DT 27-MAR-2024, entry version 86.
DE RecName: Full=Quorum-sensing regulator protein G;
DE Flags: Precursor;
GN Name=qseG; Synonyms=yfhG; OrderedLocusNames=Z3831, ECs3421;
OS Escherichia coli O157:H7.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP FUNCTION IN VIRULENCE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND GENE
RP NAME.
RC STRAIN=O157:H7 / 86-24 / EHEC;
RX PubMed=19289831; DOI=10.1073/pnas.0811409106;
RA Reading N.C., Rasko D.A., Torres A.G., Sperandio V.;
RT "The two-component system QseEF and the membrane protein QseG link
RT adrenergic and stress sensing to bacterial pathogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:5889-5894(2009).
RN [4]
RP FUNCTION IN REGULATION.
RC STRAIN=O157:H7 / 86-24 / EHEC;
RX PubMed=20056703; DOI=10.1099/mic.0.033027-0;
RA Reading N.C., Rasko D., Torres A.G., Sperandio V.;
RT "A transcriptome study of the QseEF two-component system and the QseG
RT membrane protein in enterohaemorrhagic Escherichia coli O157:H7.";
RL Microbiology 156:1167-1175(2010).
CC -!- FUNCTION: Involved in the regulation of virulence and metabolism in
CC EHEC. Required for pedestal formation in host epithelial cells during
CC infection. Necessary for translocation of effector molecules into host
CC epithelial cells, but is not involved in structural assembly of the
CC type III secretion system (T3SS). {ECO:0000269|PubMed:19289831,
CC ECO:0000269|PubMed:20056703}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane
CC {ECO:0000305|PubMed:19289831}; Lipid-anchor
CC {ECO:0000305|PubMed:19289831}.
CC -!- DISRUPTION PHENOTYPE: Mutants are unable to form pedestals.
CC {ECO:0000269|PubMed:19289831}.
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DR EMBL; AE005174; AAG57669.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36844.1; -; Genomic_DNA.
DR PIR; E91056; E91056.
DR RefSeq; NP_311448.1; NC_002695.1.
DR RefSeq; WP_001215861.1; NZ_VOAI01000001.1.
DR PDB; 7X6F; X-ray; 2.30 A; A/B/C/D/E/F=56-209.
DR PDB; 7X6G; X-ray; 2.35 A; A/B/C/D/E/F=56-209.
DR PDB; 7X6H; X-ray; 2.60 A; A/B/C/D=56-209.
DR PDBsum; 7X6F; -.
DR PDBsum; 7X6G; -.
DR PDBsum; 7X6H; -.
DR AlphaFoldDB; P0AD45; -.
DR SMR; P0AD45; -.
DR STRING; 155864.Z3831; -.
DR GeneID; 914899; -.
DR KEGG; ece:Z3831; -.
DR KEGG; ecs:ECs_3421; -.
DR PATRIC; fig|386585.9.peg.3575; -.
DR eggNOG; COG3170; Bacteria.
DR HOGENOM; CLU_068067_1_0_6; -.
DR OMA; SLYWLRA; -.
DR PHI-base; PHI:9122; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR025262; QseG.
DR Pfam; PF13942; Lipoprotein_20; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 26..237
FT /note="Quorum-sensing regulator protein G"
FT /id="PRO_0000169250"
FT REGION 201..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..237
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 26
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 26
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT HELIX 57..61
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 65..68
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 71..82
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 86..93
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 101..111
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 118..130
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 132..134
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 140..196
FT /evidence="ECO:0007829|PDB:7X6F"
FT HELIX 200..204
FT /evidence="ECO:0007829|PDB:7X6F"
SQ SEQUENCE 237 AA; 27348 MW; 594D0C495759098D CRC64;
MRHIFQRLLP RRLWLAGLPC LALLGCVQNH NKPAIDTPAE EKIPVYQLAD YLSTECSDIW
ALQGKSTETN PLYWLRAMDC ADRLMPAQSR QQARQYDDGS WQNTFKQGIL LADAKITPYE
RRQLVARIEA LSTEIPAQVR PLYQLWRDGQ ALQLQLAEER QRYSKLQQSS DSELDTLRQQ
HHVLQQQLEL TTRKLENLTD IERQLSTRKP AGNFSPDTPH ESEKPAPSTH EVTPDEP
//
