ID R0ACW2_9FIRM Unreviewed; 1122 AA.
AC R0ACW2;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=Stage 0 sporulation protein A homolog {ECO:0000256|ARBA:ARBA00018672};
DE EC=2.7.13.3 {ECO:0000256|ARBA:ARBA00012438};
GN ORFNames=HMPREF1085_02627 {ECO:0000313|EMBL:ENZ49921.1};
OS Enterocloster bolteae 90A9.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Enterocloster.
OX NCBI_TaxID=997894 {ECO:0000313|EMBL:ENZ49921.1, ECO:0000313|Proteomes:UP000013126};
RN [1] {ECO:0000313|EMBL:ENZ49921.1, ECO:0000313|Proteomes:UP000013126}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=90A9 {ECO:0000313|EMBL:ENZ49921.1,
RC ECO:0000313|Proteomes:UP000013126};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Courvalin P., Lambert T.,
RA Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B.,
RA Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., McCowan C., Murphy C., Neiman D., Pearson M.,
RA Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Clostridium bolteae 90A9.";
RL Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play the central regulatory role in sporulation. It may
CC be an element of the effector pathway responsible for the activation of
CC sporulation genes in response to nutritional stress. Spo0A may act in
CC concert with spo0H (a sigma factor) to control the expression of some
CC genes that are critical to the sporulation process.
CC {ECO:0000256|ARBA:ARBA00024867}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3; Evidence={ECO:0000256|ARBA:ARBA00000085};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENZ49921.1}.
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DR EMBL; AGYH01000006; ENZ49921.1; -; Genomic_DNA.
DR RefSeq; WP_002575806.1; NZ_KB851182.1.
DR AlphaFoldDB; R0ACW2; -.
DR PATRIC; fig|997894.4.peg.2770; -.
DR HOGENOM; CLU_000445_114_21_9; -.
DR OrthoDB; 9806130at2; -.
DR Proteomes; UP000013126; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR CDD; cd16922; HATPase_EvgS-ArcB-TorS-like; 1.
DR CDD; cd00082; HisKA; 1.
DR CDD; cd17546; REC_hyHK_CKI1_RcsC-like; 2.
DR Gene3D; 1.10.287.130; -; 1.
DR Gene3D; 3.40.50.2300; -; 2.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR Gene3D; 3.30.450.20; PAS domain; 2.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR NCBIfam; TIGR00229; sensory_box; 1.
DR PANTHER; PTHR43047:SF79; HISTIDINE KINASE; 1.
DR PANTHER; PTHR43047; TWO-COMPONENT HISTIDINE PROTEIN KINASE; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR Pfam; PF00072; Response_reg; 2.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SMART; SM00448; REC; 2.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF52172; CheY-like; 2.
DR SUPFAM; SSF47384; Homodimeric domain of signal transducing histidine kinase; 1.
DR SUPFAM; SSF55785; PYP-like sensor domain (PAS domain); 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 2.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553, ECO:0000256|PROSITE-
KW ProRule:PRU00169}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Two-component regulatory system {ECO:0000256|ARBA:ARBA00023012}.
FT TRANSMEM 12..34
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 317..340
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 499..723
FT /note="Histidine kinase"
FT /evidence="ECO:0000259|PROSITE:PS50109"
FT DOMAIN 875..997
FT /note="Response regulatory"
FT /evidence="ECO:0000259|PROSITE:PS50110"
FT DOMAIN 1004..1122
FT /note="Response regulatory"
FT /evidence="ECO:0000259|PROSITE:PS50110"
FT MOD_RES 928
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00169"
FT MOD_RES 1056
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 1122 AA; 123848 MW; 5E05532102FD01B1 CRC64;
MKQIRKKHKF SLPITITVCI ALAIVFYLSL SYFLEGYRRD IYDRIINSNI SALKETTSTL
ISKTTEGFDH CRHEIRVLGI GMSEELKENG FDSIDGLSNG DRSYLRDFNK VSVFDYCVLL
NESGRGVYSQ GDSVKPINLY SSQAYVDCLN SPDGEAISFI SDPFSASGQD VVAFSCRAGS
VILIGIYSQE SFRALYDSTA FGDNASYMIT TDSGLILSRT HVNRELGETL NLFTYFEENP
LNAEFFKPDA QGGTEYERVL SEFRNDKGGS AEIYFGDSRY ELVYAPIPRT GWEFISCVSY
DHITADAAQI NQETIQLTMF IIVLMLGMFL VIVIMLVFVV RANASREAVR RDRIFTLMTH
YVPNVIVIGD SESGAIEYAS RNTEQVLGIE DAFGNAIEEK FLSCISGSDR QAVLDLMSRI
REGECVSGSL KLHFTRPDTQ RDIVLSLNAY LIAEQDSSQR FITLTLEDIT EREQSRRRLE
EALESEARAN AAKSTFLASM SHDIRTPLNA VIGLTNLALY SPEDSKKVTE CLQKIANSSQ
LLLGLINDVL DMSKIESGKM QLAETEFELG EWLSGVVTVT QSQTGVRSQH FDVNVWNITH
ELLCGDTVRL GQVLTNVLGN AVKFTPKDGE IYLDITEVPS QDPSFASFVF RVSDTGVGMS
REYMGRIFEM FSRDQNSYKQ GIQGTGLGMA ITKRIVDLMG GTIRVESEPG KGTTFTIGLS
IRLSSRHVPL AAGCAMLVIG EPGSEEKCRD AVEKLEEIGV DAAWETDYQA AVSLAAQKRA
AGRPFSMVFL PYKMLRFDRT LTGEQLKRDL GGDTLLLLGL KPDEQELFEE IKQKGFKYTI
SLPLFRMSLY RKITGMLGQG ESISGGLAGE LKGVRLLVVE DNAVNLEIAV EMLSGLMGAA
VDTARNGEEG CTRFLDAPED TYDVILMDLQ MPVLGGLDAA RRIRESIHPQ AASIPIIALT
ANAFEEDRRE ALEAGMNGFV PKPIDFAVLA KEIGRVRREG RSLRLLLAED NELNREIAAE
LIRADGQQVD AVENGRQALE AYLNAPDGTY DAILLDLRMP VMDGFEAAAA IRGSQRKSAG
TIPVFACTSS SEDEVRSEAA RAGFGAVLTK PINMEQLRGL LG
//
