ID R0HBU3_9BRAS Unreviewed; 526 AA.
AC R0HBU3;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=tRNA(His) guanylyltransferase {ECO:0000256|ARBA:ARBA00012511};
DE EC=2.7.7.79 {ECO:0000256|ARBA:ARBA00012511};
GN ORFNames=CARUB_v10022993mg {ECO:0000313|EMBL:EOA26904.1};
OS Capsella rubella.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella.
OX NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA26904.1, ECO:0000313|Proteomes:UP000029121};
RN [1] {ECO:0000313|Proteomes:UP000029121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Monte Gargano {ECO:0000313|Proteomes:UP000029121};
RX PubMed=23749190; DOI=10.1038/ng.2669;
RA Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W., Mandakova T.,
RA Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S., Brandvain Y.,
RA Coop G., Andolfatto P., Hu T.T., Blanchette M., Clark R.M., Quesneville H.,
RA Nordborg M., Gaut B.S., Lysak M.A., Jenkins J., Grimwood J., Chapman J.,
RA Prochnik S., Shu S., Rokhsar D., Schmutz J., Weigel D., Wright S.I.;
RT "The Capsella rubella genome and the genomic consequences of rapid mating
RT system evolution.";
RL Nat. Genet. 45:831-835(2013).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the tRNA(His) guanylyltransferase family.
CC {ECO:0000256|ARBA:ARBA00010113}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KB870808; EOA26904.1; -; Genomic_DNA.
DR RefSeq; XP_006294006.1; XM_006293944.1.
DR AlphaFoldDB; R0HBU3; -.
DR STRING; 81985.R0HBU3; -.
DR eggNOG; KOG2721; Eukaryota.
DR Proteomes; UP000029121; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0008193; F:tRNA guanylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR Gene3D; 3.30.70.3000; -; 2.
DR InterPro; IPR025845; Thg1_C_dom.
DR InterPro; IPR024956; tRNAHis_GuaTrfase_cat.
DR InterPro; IPR007537; tRNAHis_GuaTrfase_Thg1.
DR InterPro; IPR038469; tRNAHis_GuaTrfase_Thg1_sf.
DR PANTHER; PTHR12729:SF6; TRNA(HIS) GUANYLYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR12729; UNCHARACTERIZED; 1.
DR Pfam; PF04446; Thg1; 2.
DR Pfam; PF14413; Thg1C; 2.
PE 3: Inferred from homology;
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000029121};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT TRANSMEM 93..110
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 6..133
FT /note="tRNAHis guanylyltransferase catalytic"
FT /evidence="ECO:0000259|Pfam:PF04446"
FT DOMAIN 140..235
FT /note="Thg1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14413"
FT DOMAIN 273..401
FT /note="tRNAHis guanylyltransferase catalytic"
FT /evidence="ECO:0000259|Pfam:PF04446"
FT DOMAIN 405..512
FT /note="Thg1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14413"
SQ SEQUENCE 526 AA; 61584 MW; C6EADBDC27037500 CRC64;
MANSKYEYVK SFEVEDEVMF PNLIVIRIDG CDFSRFSQVH QFEKPNDETA LNLMNSCASA
VLEEFPDIVF AYGYSDEFSF VFKKTSRFYQ RRASKILSLV ASFFAAVYVT KWEEFFPSRK
LEYAPSFASK VVSCASVEVL QVYLAWRQHD CHISNQYDTC FWMLVKSGKT LSETHEILKD
TQKQQRNELL FQQFGINYKM LPVLFRQGSC LFKTKVEETV KHDENGNPVK RLRRRETLVH
SENIAARSFW NEHSSLHKDL GHFPKDIGKI EPDYVKSFQF ENRLLPLTWV VLRIDGCHFH
RFSEVHEFEK PNDEKALKLM NSCAVAVLEE FQDIAFAYGV SDEYSFVLKN ESELYKRQSS
KIISAMVSFF TSKYVLRWAD FFPHKELKYP PSFDGRAVCY PTSKILLDYL AWRQVDCHTN
NQYNTCFWML VKSGKSKTQA QEYLKGTQTR EKNELLSQQF GIEYNSLPLI FRMGSSVFRL
KERVTEEKGE VSVSGKQVEE EEEVVVDHSN IIDQCFWQQH PHILSS
//
