ID R0HUK5_9BRAS Unreviewed; 846 AA.
AC R0HUK5;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=CARUB_v10025350mg {ECO:0000313|EMBL:EOA29085.1};
OS Capsella rubella.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella.
OX NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA29085.1, ECO:0000313|Proteomes:UP000029121};
RN [1] {ECO:0000313|Proteomes:UP000029121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Monte Gargano {ECO:0000313|Proteomes:UP000029121};
RX PubMed=23749190; DOI=10.1038/ng.2669;
RA Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W., Mandakova T.,
RA Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S., Brandvain Y.,
RA Coop G., Andolfatto P., Hu T.T., Blanchette M., Clark R.M., Quesneville H.,
RA Nordborg M., Gaut B.S., Lysak M.A., Jenkins J., Grimwood J., Chapman J.,
RA Prochnik S., Shu S., Rokhsar D., Schmutz J., Weigel D., Wright S.I.;
RT "The Capsella rubella genome and the genomic consequences of rapid mating
RT system evolution.";
RL Nat. Genet. 45:831-835(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000256|ARBA:ARBA00004271}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR EMBL; KB870808; EOA29085.1; -; Genomic_DNA.
DR RefSeq; XP_006296187.1; XM_006296125.1.
DR AlphaFoldDB; R0HUK5; -.
DR STRING; 81985.R0HUK5; -.
DR GeneID; 17889760; -.
DR KEGG; crb:17889760; -.
DR eggNOG; KOG0496; Eukaryota.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000029121; Unassembled WGS sequence.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd22842; Gal_Rha_Lectin_BGal; 1.
DR Gene3D; 2.60.120.740; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421:SF124; BETA-GALACTOSIDASE 2-RELATED; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 3: Inferred from homology;
KW Apoplast {ECO:0000256|ARBA:ARBA00022523};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000029121};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..846
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004341953"
FT DOMAIN 760..846
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000259|PROSITE:PS50228"
SQ SEQUENCE 846 AA; 92354 MW; 3224107AD947F14F CRC64;
MVKGKRKMEM MLLLILQIVV AATAANVTYD HRALVIDGKR KILISGSIHY PRSTPEMWPD
LIQKSKDGGL DVIETYVFWS GHEPEKNKYN FEGRYDLVKF VKLAAKAGLY VHLRIGPYVC
AEWNYGGFPV WLHFVPGIKF RTDNEPFKAE MQRFTTKIVD LMKQEKLYAS QGGPIILSQI
ENEYGNIASA YGAAGKSYIK WSASMALSLD TGVPWNMCQQ ADAPEPMINT CNGFYCDQFT
PNSNNKPKMW TENWSGWFLG FGDPSPYRPV EDLAFAVARF YQRGGTFQNY YMYHGGSNFD
RTSGGPLIST SYDYDAPIDE YGLLRQPKWG HLRDLHKAIK LCEDSLIATD PTITSLGSNL
EAAVYKTSGS CAAFLANVGT KSDATVVFNG QSYVLPAWSV SILPDCKNVA FNTAKKNAAT
ESTTFAPQSL KPDGGSSAEL GSQWSYIKEP IGISKTDAFL KPGLLEQINT TADKSDYLWY
SLRMDIKGDE TFLDEGSKAV LHIESIGQVV YAFINGKLAG SGHDKQKISL VIPINLVPGK
NTIDLLSVTV GLANYGPFFD LVGAGITGPV TLKSAKSGSS IDLATQQWTY QVGLKGEDTG
LATGDSSEWV SKSPLPTKQP LIWYKTTFNA PSGSEPVAID FTGTGKGIAW VNGQSIGRYW
PTSIAANNGC TDSCDYRGSY TAKKCVKNCG KPSQTLYHVP RSWLNPSGNT LVLLEEMGGD
PTQISFATKQ TGSSLCLTVS QSHPPPVDTW TSDSKISNKH RTRPVLSLKC PVSTHVITSI
KFASFGTPKG TCGSFTYGHC SSSRSLSLVQ KACIGLRSCN VEVSTRVFGE PCRGVVKSLA
VEASCS
//
