ID R0IBX8_9BRAS Unreviewed; 596 AA.
AC R0IBX8;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE RecName: Full=9-cis-epoxycarotenoid dioxygenase {ECO:0008006|Google:ProtNLM};
GN ORFNames=CARUB_v10008647mg {ECO:0000313|EMBL:EOA39959.1};
OS Capsella rubella.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Capsella.
OX NCBI_TaxID=81985 {ECO:0000313|EMBL:EOA39959.1, ECO:0000313|Proteomes:UP000029121};
RN [1] {ECO:0000313|Proteomes:UP000029121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Monte Gargano {ECO:0000313|Proteomes:UP000029121};
RX PubMed=23749190; DOI=10.1038/ng.2669;
RA Slotte T., Hazzouri K.M., Agren J.A., Koenig D., Maumus F., Guo Y.L.,
RA Steige K., Platts A.E., Escobar J.S., Newman L.K., Wang W., Mandakova T.,
RA Vello E., Smith L.M., Henz S.R., Steffen J., Takuno S., Brandvain Y.,
RA Coop G., Andolfatto P., Hu T.T., Blanchette M., Clark R.M., Quesneville H.,
RA Nordborg M., Gaut B.S., Lysak M.A., Jenkins J., Grimwood J., Chapman J.,
RA Prochnik S., Shu S., Rokhsar D., Schmutz J., Weigel D., Wright S.I.;
RT "The Capsella rubella genome and the genomic consequences of rapid mating
RT system evolution.";
RL Nat. Genet. 45:831-835(2013).
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
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DR EMBL; KB870805; EOA39959.1; -; Genomic_DNA.
DR RefSeq; XP_006307061.1; XM_006306999.1.
DR AlphaFoldDB; R0IBX8; -.
DR STRING; 81985.R0IBX8; -.
DR GeneID; 17898403; -.
DR KEGG; crb:17898403; -.
DR eggNOG; KOG1285; Eukaryota.
DR OrthoDB; 318119at2759; -.
DR Proteomes; UP000029121; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543:SF123; 9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED; 1.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00022964};
KW Reference proteome {ECO:0000313|Proteomes:UP000029121}.
FT REGION 25..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 294
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 343
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 408
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 583
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 596 AA; 65947 MW; 7735672675F50484 CRC64;
MACSYVLTPN PTKLNLSFAP SDLDAPSPSS SVSFTNNTRT QGRRKLISSP NSSVSGDSTP
ALLNFPNYPS PNPIIPEKDT SRWNPLQRAA SAALDFAETA LLRRERSKPL PKTVDPRHQI
SGNYAPVPEQ PVKSSISVDG KIPDCIDGVY LRNGANPLFE PVSGHHLFDG DGMVHAVKIS
NGEASYSCRF TETERLVQEK QLGSPIFPKA IGELHGHTGI ARLMLFYARG LFGLLNHKNG
TGVANAGLVY FHDRLLAMSE DDLPYQVRIT DNGDLETVGR FDFDGQLNSA MIAHPKIDPE
TKELFALSYD VVKKPYLKYF RISPDGEKSP DVEIPLASPT MMHDFAITEN FVVIPDQQVV
FQLSDMILGK SPVKYDGEKI SRFGILPRNA KDASEMVWVE SPETFCFHLW NAWESPETDE
VVVIGSCMTP ADSIFNECDE QLNSVLSEIR LNLKTGKSTR RTIIPGSVQM NLEAGMVNRN
LLGRKTRYAY LAIAEPWPKV SGFAKVDLST GEVKNHFYGG KKYGGEPFFL PRGLESDGED
DGYIMSFVHD EESWESELQI VNAVTLELEA TVKLPSRVPY GFHGTFVNSS DMLNQA
//
