ID R1F6E7_9GAMM Unreviewed; 1158 AA.
AC R1F6E7;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=G113_09422 {ECO:0000313|EMBL:EOD55342.1};
OS Aeromonas molluscorum 848.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=1268236 {ECO:0000313|EMBL:EOD55342.1, ECO:0000313|Proteomes:UP000013526};
RN [1] {ECO:0000313|EMBL:EOD55342.1, ECO:0000313|Proteomes:UP000013526}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=848 {ECO:0000313|EMBL:EOD55342.1,
RC ECO:0000313|Proteomes:UP000013526};
RX PubMed=23788549;
RA Spataro N., Farfan M., Albarral V., Sanglas A., Loren J.G., Fuste M.C.,
RA Bosch E.;
RT "Draft Genome Sequence of Aeromonas molluscorum Strain 848TT, Isolated from
RT Bivalve Molluscs.";
RL Genome Announc. 1:E00382-13(2013).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOD55342.1}.
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DR EMBL; AQGQ01000050; EOD55342.1; -; Genomic_DNA.
DR RefSeq; WP_005899483.1; NZ_AQGQ01000050.1.
DR AlphaFoldDB; R1F6E7; -.
DR PATRIC; fig|1268236.3.peg.1859; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000013526; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 626..787
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 809..962
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1158 AA; 129404 MW; 92E8C59E7FE12E55 CRC64;
MRSLTLPVLP AKAGQKSTLG QLHGSALALV AAELTRSNDG PTLLVTADTP SALRLEHELR
YLLSQVEPGE QACPVLLFPD WETLPYDTFS PHQDIISQRL ETLYKLPQMQ RGALIVPIST
LMLRCAPRVY LDKYSLMVKA GQRLNLQQLR GRLAEAGYVA VEQVLEHGEF AARGSLLDLF
PMGSTSPYRI DFFDDEVDSI RPFDPESQRS REPVPVVSLL PAREYPTDEA AIELFRGQFR
EQFELSRADG SIYQEVSKGR WPAGIEYYLP LFFEQTASVF DYLPVQTQLI TVGDIYPAAQ
RFWNDIGQRY EERRWDTSRP LLEPAYLYLP VEQLFQALGQ YGAVRLQEEP IIQGAGQYNL
PFEPLPELQL DNSKERPLLA LEQFLQDFAG VTLFAVESEG RREVLRDLLA RIGLNPPEFA
SLSEFAASPS AQGILVGPLE RGFLLREPKV APLAFITEGE LLGGKIRSKR QRDKSKNLSS
EAVIRNLGEL TQGQPVVHLD HGVGRYLGLE TLDAGGMPTE FLTLEYAGGD KLFVPVTNLH
LISRYTGSDN PPSHKLGGEA WVKARRKAAE KVRDVAAELL DVYALRAARK GFAFKHDKES
YRQFAAGFPF EETEDQLNAI NAVLGDMCQA KSMDRLVCGD VGFGKTEVAM RAAFVAVHGG
KQVAVLVPTT LLAQQHYDNF RDRFANWPVR VEVLSRFRSA KEQTAVLKEL RDGKVDIIIG
THKLLGSDLT FKDLGLLIVD EEHRFGVRQK EKIKALRADV DILTLTATPI PRTLNMAMAG
MRDLSIIATP PAKRLAIKTF VRQQEPAVTR EAVLRELKRG GQIYYLHNDV ASIDKCASDL
AELIPEARIG IAHGQMPERD LERIMSDFYH QRFNLLVCTT IIETGIDVPS ANTIIMDRAD
HLGLAQLHQL RGRVGRSHHQ AYAYLLTPNP KLMTKDAAKR LDAIASLEDL GAGFALATHD
LEIRGAGELL GDDQSGQIES VGFTLYMEML EQAVEALKSG KEPSLEQLMS DHTEIELRLP
ALLPEGYIPD VNMRLSMYKR IASASDEAEL RELKVELIDR FGLLPDAAKT LFDIASFRQQ
AAALGIKRLE MSERGGYLDF TQETRVNPGY LVKLLSEQPR VFKMDGPTRL RFQVPTQDRV
TRLQRVEGLL KDLAAHSG
//
