ID   R1GCS8_BOTPV            Unreviewed;       901 AA.
AC   R1GCS8;
DT   26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2013, sequence version 1.
DT   24-JAN-2024, entry version 41.
DE   SubName: Full=Putative major intrinsic protein {ECO:0000313|EMBL:EOD46011.1};
GN   ORFNames=UCRNP2_7217 {ECO:0000313|EMBL:EOD46011.1};
OS   Botryosphaeria parva (strain UCR-NP2) (Grapevine canker fungus)
OS   (Neofusicoccum parvum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC   Neofusicoccum.
OX   NCBI_TaxID=1287680 {ECO:0000313|EMBL:EOD46011.1, ECO:0000313|Proteomes:UP000013521};
RN   [1] {ECO:0000313|Proteomes:UP000013521}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCR-NP2 {ECO:0000313|Proteomes:UP000013521};
RX   PubMed=23766404; DOI=10.1128/genomea.00339-13;
RA   Blanco-Ulate B., Rolshausen P., Cantu D.;
RT   "Draft genome sequence of Neofusicoccum parvum isolate UCR-NP2, a fungal
RT   vascular pathogen associated with grapevine cankers.";
RL   Genome Announc. 1:E0033913-E0033913(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O(in) = H2O(out); Xref=Rhea:RHEA:29667, ChEBI:CHEBI:15377;
CC         Evidence={ECO:0000256|ARBA:ARBA00034651};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR   EMBL; KB916504; EOD46011.1; -; Genomic_DNA.
DR   RefSeq; XP_007586473.1; XM_007586411.1.
DR   AlphaFoldDB; R1GCS8; -.
DR   STRING; 1287680.R1GCS8; -.
DR   ESTHER; botpv-r1gcs8; Fungal_carboxylesterase_lipase.
DR   KEGG; npa:UCRNP2_7217; -.
DR   eggNOG; KOG0223; Eukaryota.
DR   eggNOG; KOG4389; Eukaryota.
DR   HOGENOM; CLU_321584_0_0_1; -.
DR   OrthoDB; 3236018at2759; -.
DR   Proteomes; UP000013521; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015267; F:channel activity; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   Gene3D; 1.20.1080.10; Glycerol uptake facilitator protein; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR023271; Aquaporin-like.
DR   InterPro; IPR034294; Aquaporin_transptr.
DR   InterPro; IPR002018; CarbesteraseB.
DR   InterPro; IPR019819; Carboxylesterase_B_CS.
DR   InterPro; IPR000425; MIP.
DR   PANTHER; PTHR19139:SF199; AQUAPORIN; 1.
DR   PANTHER; PTHR19139; AQUAPORIN TRANSPORTER; 1.
DR   Pfam; PF00135; COesterase; 1.
DR   Pfam; PF00230; MIP; 1.
DR   PRINTS; PR00783; MINTRINSICP.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR   SUPFAM; SSF81338; Aquaporin-like; 1.
DR   PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE   4: Predicted;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000013521};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        54..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        87..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        115..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        140..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        173..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        203..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        248..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          380..741
FT                   /note="Carboxylesterase type B"
FT                   /evidence="ECO:0000259|Pfam:PF00135"
SQ   SEQUENCE   901 AA;  96031 MW;  79BCAC9781958548 CRC64;
     MNINKPADNP IVLPGMRVKT SERPPPRRVH GLSWLPTKAR NHIIAMIGEF TGTFLFLFFA
     FAATQVANAA AAFAATKDGS VNKYPNVPVL LYISLAFGFS LAVNVWVFFR ISGGLFNPAV
     TLGLCLIGAV GWIRGFLVII TQIVGAIAAA GIVSCLFPGP LNVRTTLGAE TSVVRGLFIE
     MFLTAQLIFT IFMLAAEKHK GTFLAPVGIG LCLFMTELGG IYFTGGSLNP ARSFGPDVIL
     HTFDGYHWIY WVGPALGAIL AVALYRLIKA LEYETANPGA DFNKGDAEVF YPDEDPHAGR
     RGPSSSGYDV SHYRNAVDGV SEAEMPKYEN EHKERTLSKP IGLTGPSTTI KGLTVLPMEK
     EGWAHGRTLG LETPSDGGLP ILNLPYARIQ AKTYDQENDI YIFKNIPFAA PPTGENRFAK
     PQPPLQNDTL SDGSYGPNCV QFLTAIFSGS ASSSNSSASG GIGSLLGGLL GGSSGLTTNE
     TALEELFASL FGPSSEDCLF LDVFVPGKAV KAPNTANLPV VHWGAYVLGS KNMNNGTGIV
     TAGDNSVIFV ASNYRVNGFG FLAGSTMETE GTPNAGLHDT HAAFNWTRSY ISLLGGNPNQ
     VSAWGESAGA GSLSALLTAY GGTQDPLFNT AVLLSPALDM EWDRTGTLEQ RFQNFSAAAG
     CAGQGVECLR KAPLEALKAG NQALLTSPYA GRPPYQPATD GSLLPHLPTV SYGTGAHWPN
     LTALILSHTA DEATIFLDRT ITTWPAFAAY VRYFWPPPAV AARITTRFRT AHPASPQAAL
     HALIEQSRVT CNVRAAADAF NTSAAVYNLQ YAVPPALHGE DIAPTFSQPS RLLPLQGYQT
     AYQSYLVSLA RSEVGDPNEW RERLSLPPTV EWPRVVDVAG GFFGGVLDSL HVQGSSHLSA
     S
//