ID   R2RUC8_9ENTE            Unreviewed;       533 AA.
AC   R2RUC8;
DT   26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2013, sequence version 1.
DT   24-JAN-2024, entry version 30.
DE   SubName: Full=Xylan 1,4-beta-xylosidase {ECO:0000313|EMBL:EOH86915.1};
GN   ORFNames=UAS_01378 {ECO:0000313|EMBL:EOH86915.1};
OS   Enterococcus asini ATCC 700915.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=1158606 {ECO:0000313|EMBL:EOH86915.1, ECO:0000313|Proteomes:UP000013777};
RN   [1] {ECO:0000313|EMBL:EOH86915.1, ECO:0000313|Proteomes:UP000013777}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700915 {ECO:0000313|EMBL:EOH86915.1,
RC   ECO:0000313|Proteomes:UP000013777};
RG   The Broad Institute Genome Sequencing Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A.M., Gilmore M.S., Lebreton F., Walker B., Young S.K., Zeng Q.,
RA   Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA   Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA   Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA   Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA   Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT   "The Genome Sequence of Enterococcus asini ATCC_700915.";
RL   Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EOH86915.1}.
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DR   EMBL; AJAP01000012; EOH86915.1; -; Genomic_DNA.
DR   RefSeq; WP_010754019.1; NZ_KB946294.1.
DR   AlphaFoldDB; R2RUC8; -.
DR   STRING; 57732.RU94_GL001499; -.
DR   GeneID; 78365383; -.
DR   PATRIC; fig|1158606.3.peg.1334; -.
DR   eggNOG; COG3507; Bacteria.
DR   HOGENOM; CLU_016508_2_1_9; -.
DR   OrthoDB; 9801455at2; -.
DR   Proteomes; UP000013777; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd09000; GH43_SXA-like; 1.
DR   Gene3D; 2.60.120.200; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR041542; GH43_C2.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR   Pfam; PF17851; GH43_C2; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR   SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000013777}.
FT   DOMAIN          327..510
FT                   /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT                   /evidence="ECO:0000259|Pfam:PF17851"
FT   ACT_SITE        17
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        188
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            128
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   533 AA;  60072 MW;  8234D7C35DCC7BBE CRC64;
     MYKTSISNPI LRGFNPDPNI LKVEDTYYIA VSSFEWLPGI RVYASQDLVN WAHETDILTD
     QVHLQGNPKN CSIWAPQISY HAGRYYCIYT DVKSSSRPFK DCHNYLITAS SIKGPWSEPV
     YLNSSGFDPS LFHDEDGRSW LLNEIWDYRM TTGNKSAGIV MQEYDDKKQC LVGPRYDIFR
     GTHLAKTEAP HLYQKNGYYY LITAEGGTGT GHAVTICRSK QITGPYELAP NSPILTAADK
     PESPLQCSGH GSLVEGAFGK WYMVYLTTRP LQGHAILGRE TAIQEVYWTE DDWLALASGG
     NGPQQETIVY TKEPVIQQIT TDFATDFRGP LEKEWNSPRI MPAESWVQTG PEGLCMTSGE
     SPQSRFNHHL LALRQKDQSY EVTTRFSFNP TTYNQMAGLM LYLNEDAYLY AFVTYDEEKG
     KVLRFMRCQG EGVVTPICVP LSEQELSLKV VDNAGQAQFY YRESGSWQSF GQLEDTLFLS
     GGFTGNFIGV AVHDLDLKAG SKAYFQEFTY LGKDSKPQVN QGISKSEEMI IIP
//