ID R2XML2_9ENTE Unreviewed; 470 AA.
AC R2XML2;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=6-phospho-beta-galactosidase {ECO:0000256|RuleBase:RU004469};
DE EC=3.2.1.85 {ECO:0000256|RuleBase:RU004469};
GN ORFNames=UKC_02031 {ECO:0000313|EMBL:EOI56134.1};
OS Enterococcus gilvus ATCC BAA-350.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1158614 {ECO:0000313|EMBL:EOI56134.1, ECO:0000313|Proteomes:UP000013750};
RN [1] {ECO:0000313|EMBL:EOI56134.1, ECO:0000313|Proteomes:UP000013750}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-350 {ECO:0000313|EMBL:EOI56134.1,
RC ECO:0000313|Proteomes:UP000013750};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Gilmore M.S., Lebreton F., Walker B., Young S.K., Zeng Q.,
RA Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Enterococcus gilvus ATCC BAA-350.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 6-phospho-beta-D-galactoside + H2O = an alcohol + D-
CC galactose 6-phosphate; Xref=Rhea:RHEA:24568, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:30879, ChEBI:CHEBI:58534, ChEBI:CHEBI:91004; EC=3.2.1.85;
CC Evidence={ECO:0000256|RuleBase:RU004469};
CC -!- PATHWAY: Carbohydrate metabolism; lactose degradation; D-galactose 6-
CC phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1.
CC {ECO:0000256|RuleBase:RU004469}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 1 family.
CC {ECO:0000256|ARBA:ARBA00010838}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EOI56134.1}.
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DR EMBL; AJDQ01000007; EOI56134.1; -; Genomic_DNA.
DR RefSeq; WP_010780427.1; NZ_KB946874.1.
DR AlphaFoldDB; R2XML2; -.
DR PATRIC; fig|1158614.3.peg.2041; -.
DR eggNOG; COG2723; Bacteria.
DR HOGENOM; CLU_001859_1_3_9; -.
DR OrthoDB; 9765195at2; -.
DR UniPathway; UPA00542; UER00605.
DR Proteomes; UP000013750; Unassembled WGS sequence.
DR GO; GO:0033920; F:6-phospho-beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0019512; P:lactose catabolic process via tagatose-6-phosphate; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR005928; 6P-beta-galactosidase.
DR InterPro; IPR001360; Glyco_hydro_1.
DR InterPro; IPR018120; Glyco_hydro_1_AS.
DR InterPro; IPR033132; Glyco_hydro_1_N_CS.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR NCBIfam; TIGR01233; lacG; 1.
DR PANTHER; PTHR10353:SF340; 6-PHOSPHO-BETA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR10353; GLYCOSYL HYDROLASE; 1.
DR Pfam; PF00232; Glyco_hydro_1; 1.
DR PRINTS; PR00131; GLHYDRLASE1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00572; GLYCOSYL_HYDROL_F1_1; 1.
DR PROSITE; PS00653; GLYCOSYL_HYDROL_F1_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU004468};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU004468}.
FT ACT_SITE 378
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10055"
SQ SEQUENCE 470 AA; 53529 MW; 68F1B4A3FC422D67 CRC64;
MLNRVKELPK GFVWGAATAA FQVEGATSIE GKGKTMWDDY LIEQGRFLPD PACDFYHRYE
EDVKLAKESG LDALRISIAW SRIFPDGTGE PNPLGVDYYH RVFAACHENS IEPYVTLHHF
DSPKAIFDKG DWLNRENIEA FVYYAEYCFK EFSEVENWFT INELFALAMG QYITGIFPPC
HHFDVTSAIQ AQHNELLAHA KAVNRFKELG IPGKIGLIHS LHPVYPLTDS EEDKHAAKLT
DAFRNRFLLD GSFLGYYSED TMEAINEILS LNDAELEILP GDLDILARAA IQNDYFGLNY
YQNDFVKSYE GESGNTFNGT GEKGTSSALR FKGMGQIVKK PGIPTTDWDW NIFPQGMYDI
LKDVSDSYPN CPTIYVTENG LGYKDVLKTD GTVCDDERID FIDQHVAAIL QARADGVDVE
GYFVWSLQDQ FSWANGYNKR YGLVYIDFDT QKRYLKKSAF WFKKLSDTRS
//
