ID R4U700_9MOLU Unreviewed; 474 AA.
AC R4U700;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000256|HAMAP-Rule:MF_00945};
GN Name=nusA {ECO:0000256|HAMAP-Rule:MF_00945,
GN ECO:0000313|EMBL:AGM26413.1};
GN ORFNames=SSYRP_v1c08240 {ECO:0000313|EMBL:AGM26413.1};
OS Spiroplasma syrphidicola EA-1.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=1276229 {ECO:0000313|EMBL:AGM26413.1, ECO:0000313|Proteomes:UP000013963};
RN [1] {ECO:0000313|EMBL:AGM26413.1, ECO:0000313|Proteomes:UP000013963}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EA-1 {ECO:0000313|EMBL:AGM26413.1};
RX PubMed=23711669; DOI=10.1093/gbe/evt084;
RA Ku C., Lo W.S., Chen L.L., Kuo C.H.;
RT "Complete genomes of two dipteran-associated spiroplasmas provided insights
RT into the origin, dynamics, and impacts of viral invasion in spiroplasma.";
RL Genome Biol. Evol. 5:1151-1164(2013).
CC -!- FUNCTION: Participates in both transcription termination and
CC antitermination. {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC dependent RNA polymerase and to nascent RNA. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00945}.
CC -!- SIMILARITY: Belongs to the NusA family. {ECO:0000256|HAMAP-
CC Rule:MF_00945}.
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DR EMBL; CP005078; AGM26413.1; -; Genomic_DNA.
DR RefSeq; WP_016341054.1; NC_021284.1.
DR AlphaFoldDB; R4U700; -.
DR STRING; 1276229.SSYRP_v1c08240; -.
DR KEGG; ssyr:SSYRP_v1c08240; -.
DR PATRIC; fig|1276229.3.peg.819; -.
DR eggNOG; COG0195; Bacteria.
DR HOGENOM; CLU_029242_2_2_14; -.
DR OrthoDB; 9807233at2; -.
DR Proteomes; UP000013963; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-UniRule.
DR GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR CDD; cd02134; KH-II_NusA_rpt1; 1.
DR CDD; cd22529; KH-II_NusA_rpt2; 1.
DR Gene3D; 3.30.300.20; -; 2.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.30.1480.10; NusA, N-terminal domain; 1.
DR HAMAP; MF_00945_B; NusA_B; 1.
DR InterPro; IPR015946; KH_dom-like_a/b.
DR InterPro; IPR025249; KH_dom_NusA-like.
DR InterPro; IPR009019; KH_sf_prok-type.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR030842; NusA_bac.
DR InterPro; IPR036555; NusA_N_sf.
DR InterPro; IPR013735; TF_NusA_N.
DR InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR NCBIfam; TIGR01953; NusA; 1.
DR PANTHER; PTHR22648; TRANSCRIPTION TERMINATION FACTOR NUSA; 1.
DR PANTHER; PTHR22648:SF0; TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA; 1.
DR Pfam; PF13184; KH_5; 1.
DR Pfam; PF08529; NusA_N; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF54814; Prokaryotic type KH domain (KH-domain type II); 2.
DR SUPFAM; SSF69705; Transcription factor NusA, N-terminal domain; 1.
DR PROSITE; PS50084; KH_TYPE_1; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00945};
KW Elongation factor {ECO:0000313|EMBL:AGM26413.1};
KW Protein biosynthesis {ECO:0000313|EMBL:AGM26413.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000013963};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription antitermination {ECO:0000256|HAMAP-Rule:MF_00945};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00945};
KW Transcription termination {ECO:0000256|ARBA:ARBA00022472,
KW ECO:0000256|HAMAP-Rule:MF_00945}.
FT DOMAIN 7..127
FT /note="Transcription factor NusA N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08529"
FT DOMAIN 232..299
FT /note="KH"
FT /evidence="ECO:0000259|Pfam:PF13184"
FT REGION 444..474
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 361..392
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 450..474
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 474 AA; 54159 MW; 48F4DBCFE0E15AE8 CRC64;
MIDGAKLLTT IEEIVSEKQI PRELILESIK EGFKKAYEKH FDPEAIIEVE IDQQTGQIKV
TKELTIVKKV EDDLLEVGLN EAKKRFGDNV TIGDKVYEDV DSAEFSRLAI IQVGQIIKQQ
IKEAEKDSIF EEYIIQKGHL MNGIVIAVEE KFVLVEVERT FAYIPAKNLI FSDHYEVGDR
ILFLAEDIVK SKNAGQITGT RTNNEFLFRL LEREIPEIFE KTIEVKAIAR DPGRRSKIAV
YSENKNIDPI GACVGTKGNR INKVTAELQN EKIDICIYDE NEQQFIINSL SPVKVISITT
TPENKEADIV VPDEQLSLAI GKGGSAAKLV AKLTKWKLNI ISYSEALNRN LEILWNGNIN
LGELEELKIR LKDKVKEKDK NYQAKLQQAA EQFEPEPFEI IEEQLVEAEE IKVLPEDGFH
VEDLSNVEVP PTVDLTEEIQ TIEDNLSYFE DSEKPEEDEE EINYDDYDEY YDEK
//
