ID R4UET8_9MOLU Unreviewed; 615 AA.
AC R4UET8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=protein-N(pi)-phosphohistidine--D-fructose phosphotransferase {ECO:0000256|ARBA:ARBA00012799};
DE EC=2.7.1.202 {ECO:0000256|ARBA:ARBA00012799};
GN Name=manY {ECO:0000313|EMBL:AGM26444.1};
GN ORFNames=SSYRP_v1c08550 {ECO:0000313|EMBL:AGM26444.1};
OS Spiroplasma syrphidicola EA-1.
OC Bacteria; Mycoplasmatota; Mollicutes; Entomoplasmatales; Spiroplasmataceae;
OC Spiroplasma.
OX NCBI_TaxID=1276229 {ECO:0000313|EMBL:AGM26444.1, ECO:0000313|Proteomes:UP000013963};
RN [1] {ECO:0000313|EMBL:AGM26444.1, ECO:0000313|Proteomes:UP000013963}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EA-1 {ECO:0000313|EMBL:AGM26444.1};
RX PubMed=23711669; DOI=10.1093/gbe/evt084;
RA Ku C., Lo W.S., Chen L.L., Kuo C.H.;
RT "Complete genomes of two dipteran-associated spiroplasmas provided insights
RT into the origin, dynamics, and impacts of viral invasion in spiroplasma.";
RL Genome Biol. Evol. 5:1151-1164(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose(out) + N(pros)-phospho-L-histidyl-[protein] = D-
CC fructose 1-phosphate(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:49252, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:29979, ChEBI:CHEBI:37721, ChEBI:CHEBI:58674,
CC ChEBI:CHEBI:64837; EC=2.7.1.202;
CC Evidence={ECO:0000256|ARBA:ARBA00001401};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; CP005078; AGM26444.1; -; Genomic_DNA.
DR RefSeq; WP_016341084.1; NC_021284.1.
DR AlphaFoldDB; R4UET8; -.
DR STRING; 1276229.SSYRP_v1c08550; -.
DR KEGG; ssyr:SSYRP_v1c08550; -.
DR PATRIC; fig|1276229.3.peg.850; -.
DR eggNOG; COG1299; Bacteria.
DR eggNOG; COG1445; Bacteria.
DR HOGENOM; CLU_013155_0_0_14; -.
DR OrthoDB; 9782569at2; -.
DR Proteomes; UP000013963; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0005351; F:carbohydrate:proton symporter activity; IEA:InterPro.
DR GO; GO:0022877; F:protein-N(PI)-phosphohistidine-fructose phosphotransferase system transporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR CDD; cd05569; PTS_IIB_fructose; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR InterPro; IPR003353; PTS_IIB_fruc.
DR InterPro; IPR006327; PTS_IIC_fruc.
DR NCBIfam; TIGR00829; FRU; 1.
DR NCBIfam; TIGR01427; PTS_IIC_fructo; 1.
DR PANTHER; PTHR30505; FRUCTOSE-LIKE PERMEASE; 1.
DR PANTHER; PTHR30505:SF0; FRUCTOSE-LIKE PTS SYSTEM EIIBC COMPONENT-RELATED; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; PTS system IIB component-like; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000013963};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 132..153
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 277..301
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 321..340
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 347..365
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 380..399
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 442..461
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 481..504
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 8..103
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000259|PROSITE:PS51099"
FT DOMAIN 128..498
FT /note="PTS EIIC type-2"
FT /evidence="ECO:0000259|PROSITE:PS51104"
SQ SEQUENCE 615 AA; 66590 MW; 3A93836BA09F5F81 CRC64;
MTTDKIKIVA ITACAAGVAH TYMAADSLKK AASEKGFEIN VETQGTIGAE NVLTAEQIAQ
ADLVIIAADV KIDLSRFNDK KLFKTGVNFA IKNPAQLIDT ALEQGTIHGK KGTKVGAVTF
GTTKKRGMLS HLMTGISWMI PMTIAAGILM AIANLCAFQT EYVDNYGDYG QNWVLGTRKL
PQFMMDVGAL GFKLMIPLFA GFTAYSIADK PGLAPAMIAG WIINDNKMLG IDVTVGTTNS
PIEPGAGFIG AIVVGLLSGY LVQLLKIIRW PKVLQPIVPL MIIPILATFI MATFVKFVIG
APIAGLVLGM FDGLSALQEK FASTGIIIAI VVAIMIAFDL GGPLNKTALV FGTVIFYQTL
AQAMAEGKTF WDANFIPGTA TQAAISVPPL GMWLSTIIFK NRYPKNQRIM GKAAFGTGMV
GITEGAIPFA AADPIRIIPA NIAGSVVAAI LVTLWDVKFA AGLGSPLGTF LGYIQQNSAP
FWPMAWLLAI LAGVVTTAVI AGLLKPKIKG EEAQLLKEEK LSRKIARKNA KNNLKLLFTK
PKLAGKKMNH WFKRTWHNRI KSTKEYWIWI GTGIKNYFVD WGYSIQRGWI SFTNLFRRKK
NKKDPSVVGV KIKKD
//
