ID R5B3B9_9BACE Unreviewed; 694 AA.
AC R5B3B9;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0000256|ARBA:ARBA00012541};
DE EC=2.4.1.18 {ECO:0000256|ARBA:ARBA00012541};
GN ORFNames=BN813_01853 {ECO:0000313|EMBL:CCX48709.1};
OS Bacteroides sp. CAG:927.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=1262753 {ECO:0000313|EMBL:CCX48709.1, ECO:0000313|Proteomes:UP000017907};
RN [1] {ECO:0000313|EMBL:CCX48709.1, ECO:0000313|Proteomes:UP000017907}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:927 {ECO:0000313|Proteomes:UP000017907};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000256|ARBA:ARBA00000826};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000256|ARBA:ARBA00009000}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCX48709.1}.
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DR EMBL; CAWM010000075; CCX48709.1; -; Genomic_DNA.
DR AlphaFoldDB; R5B3B9; -.
DR STRING; 1262753.BN813_01853; -.
DR Proteomes; UP000017907; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:InterPro.
DR CDD; cd11321; AmyAc_bac_euk_BE; 1.
DR CDD; cd02854; E_set_GBE_euk_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000017907};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 193..573
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 341
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
FT ACT_SITE 395
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
SQ SEQUENCE 694 AA; 79978 MW; D9E9C6282B5D562B CRC64;
MPIPKKAPKK PKAEKLLPLV KNDPYLMPFA AAIAGRHEDA VRKEKELTSE CGSLKDLANG
HEFFGMHRNA DGSWTFREWA PNATRIVLIG DFSDWKALAR YDLERKDNGV WEINLPADAI
HHGDYYKMLV SWQGGEGERI PAYATRVVQD PTTHLFSAQV WEPEHEYEWK VAEFKPDTKP
LLIYECHIGM AQEREDVGTY AEFRDLILPR IARDGYNAIQ IMAIQEHPYY GSFGYHVSNF
FAPSSRFGTP EDLKSLIDTA HSMGIAVIMD IVHSHAVKNE LEGLGRLDGS YNQYFYGDGR
REHPAWDSLC FDYGKNEVLH FLLSNCKYWM EEFRFDGFRF DGVTSMLYYN HGLGQAFGSY
DDYYNGNQDT NAITYLTLAN KLIHSLNPYA ITIAEEMSGM PGLAIPFKDG GIGFDYRMAM
GIPDYWIKTL KEKKDEDWHP TSIFWELTNR RDDEKTISYV ESHDQALVGD KTVIFRLIDK
EMYWHMNKDD DNGVVARGMA LHKMIRLVTA ATINGGYLNF MGNEWGHPEW IDFPREGNGW
SYKYARRLWS LVDNGFLKYE FLNNFDNAMI HLISSVYNFQ SLPVGKLWEK DDDQVLAFSR
GDLIFVFNWH PFKSFDSYGI LTPPGEYEVV LSSDNPDFGG YGNVDESVHH FTIHDDLYSP
IGVEWLKLYL PSRSAMVLRR KKQSRPRKST SAKK
//
