ID R5M7J8_9CLOT Unreviewed; 655 AA.
AC R5M7J8;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=Fe-S oxidoreductase {ECO:0000313|EMBL:CCY85579.1};
GN ORFNames=BN500_02207 {ECO:0000313|EMBL:CCY85579.1};
OS Clostridium sp. CAG:149.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262776 {ECO:0000313|EMBL:CCY85579.1, ECO:0000313|Proteomes:UP000017912};
RN [1] {ECO:0000313|EMBL:CCY85579.1, ECO:0000313|Proteomes:UP000017912}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:149 {ECO:0000313|Proteomes:UP000017912};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCY85579.1}.
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DR EMBL; CAYX010000259; CCY85579.1; -; Genomic_DNA.
DR AlphaFoldDB; R5M7J8; -.
DR Proteomes; UP000017912; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000017912}.
FT DOMAIN 1..151
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 223..453
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 524..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 655 AA; 75572 MW; 7622006C434002B2 CRC64;
MKFLLAAVNA KYIHSNLGIY SLKKYADSRL KQSGEKQEKE PSGGGPVWEI EIGEYTINHR
TDDILRDIYR RAPDAVGFSC YIWNILYIRE LVHDLKKILP DTEIWLGGPE VSYRASELLR
EEPYVRGIMA GEGEETFYRL LRAAGERTSA GSIWKDQALS LIPGLVFRAL GADGENREDG
KNRKDGENRE DGKVRAEAEI VTTPPAPLLD LNEIPFAYGD LQGLEHRIIY YESSRGCPFS
CSYCLSSIDK SVRFRDLELV KEELSFFLEK KVPQVKFVDR TFNCRKSHSM AVWQFILEND
NGITNFHFEI SADLLSEEEL ALLSKMRPGL VQLEIGVQTT NPRTIKEIRR KMDLGRLQKN
VEKIGSFHNI HQHLDLIAGL PFEGFESFRH SFNEVFAMRP EQLQLGFLKV LSGSHMMEMA
AEYSLRYKEA PPYEVLSTRW LSYGELLRLK GVEEMVETYY NSGQFENTLE ALAQEFDSPF
DMFSALSVYY EENGLSAVSH SRLARYEILF RFIGELLEKK RARYEQKTPE KEGGDEAPRE
LPAGQERLEE YRDRLILDVY LRENAKSRPS FARDLSPFRE AVKAFFIEEG KSRRYLKGYE
EYDSRQMGKM AHIEVLREGT MVLFDYLHRD ALLGNAKIFQ VGQLGEVRRQ EEAEV
//
