ID R5NH60_9FIRM Unreviewed; 563 AA.
AC R5NH60;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:CCZ03485.1};
GN ORFNames=BN730_00321 {ECO:0000313|EMBL:CCZ03485.1};
OS Eubacterium sp. CAG:603.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=1262891 {ECO:0000313|EMBL:CCZ03485.1, ECO:0000313|Proteomes:UP000018312};
RN [1] {ECO:0000313|EMBL:CCZ03485.1, ECO:0000313|Proteomes:UP000018312}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:603 {ECO:0000313|Proteomes:UP000018312};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ03485.1}.
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DR EMBL; CAZI010000022; CCZ03485.1; -; Genomic_DNA.
DR AlphaFoldDB; R5NH60; -.
DR Proteomes; UP000018312; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR025288; DUF4080.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13311; DUF4080; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000018312}.
FT DOMAIN 1..134
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 171..401
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 563 AA; 66979 MW; 7DD329AD7A3FD23C CRC64;
MKILLVAINA KYIHSNLAVY CLKKYSKEYD NNIEIAEYTI NQQMEFILQD IYKRNADVVA
FSCYIWNIEY VTAIIKDLKK IMKNNDIWVG GPEVSYRATS FLEEYKEVKG VMIGEGEKSF
NRLTKEYVED TKDFSNIKGV VYRDGEELKA TPCEELLDLS DVPFPYDDLD KFKNKIIYYE
SSRGCPYSCS YCLSSIDKKL RFRNLELVKS ELKIFIDHKI PQVKFVDRTF NCNKKHAMEI
WRFINENDNG VTNFHFEISA DIIDEEELAL FKTMRPGLIQ LEIGVQTTNP VTIKEIDRIM
NLDKLRYVVE KINSFNNIHQ HLDLIAGLPY EDYNSFVKSF NDVYAMRPEQ LQLGFLKVLS
GAKMERKSEE YGLVYTTLSP YEVLTTKWLD YDRVLELKTI ENMVEIYYNS RQFTNALAWL
ENQFETPFKM FEGIGKYYEL HKLNEVKHNR IQMYEILLEY VKSVCEDYKV FEELLKLDVY
LRENIKSRPA FAGDNHKEEL RDYYHEYRSE GKNIHIELFN INIRKWINNR TIEQTEQVVM
FDYREKDPIT LWCRLNYLTD KTI
//
