ID   R5PFD1_9BACT            Unreviewed;       614 AA.
AC   R5PFD1;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   SubName: Full=Glycoside hydrolase family 43 {ECO:0000313|EMBL:CCZ14803.1};
GN   ORFNames=BN679_01671 {ECO:0000313|EMBL:CCZ14803.1};
OS   Prevotella sp. CAG:487.
OC   Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC   Prevotella.
OX   NCBI_TaxID=1262928 {ECO:0000313|EMBL:CCZ14803.1, ECO:0000313|Proteomes:UP000018275};
RN   [1] {ECO:0000313|EMBL:CCZ14803.1, ECO:0000313|Proteomes:UP000018275}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:487 {ECO:0000313|Proteomes:UP000018275};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC       {ECO:0000256|ARBA:ARBA00004834}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CCZ14803.1}.
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DR   EMBL; CAZM010000234; CCZ14803.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5PFD1; -.
DR   Proteomes; UP000018275; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd18616; GH43_ABN-like; 1.
DR   CDD; cd08991; GH43_HoAraf43-like; 1.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR   PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 2.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 2.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000313|EMBL:CCZ14803.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018275};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..614
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004400034"
FT   ACT_SITE        36
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        197
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            150
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   614 AA;  69112 MW;  F4BC0EF94C5E622D CRC64;
     MRKLRILAAS LLLSASVCTF AGDTYTNPVI NTSLPDPTVI RADDGYFYLY ATEDIRNLPI
     YRSRDLTDWQ FVGTAFTDDT RPQWNKKGNM WAPDINKIGD KYVLYYSKSE WGGEWTCGIG
     AATADRPEGP FTDHGPLFIS SEIGVRNSID QFYIEDNGHK YLFWGSFHGI YGIELSDDGL
     SVKPGAVKKQ VSGTFMEGTY IHKRGKYYYL FGSAGTCCDG ARSTYRVTYG RSENLFGPYV
     DKKGQRLLDN HYEVMLHGDD TFVGTGHNAE FVTDDLGQDW ILYHGYKKAE ADDGRVVFLS
     RVDWKDGWPE VAGSVPEKEN VKPSFGQIHL ADPTVFCDNG TYYLYGTSPV SDNGFWVYTS
     TDLQHWSGPA GVVDGYALRG NTYGTQGFWA PQVFKKDGRY AMAYTANEQI AIAWADSPLG
     PFVQDEPAMI PAKTKEIDPF VFHDDDGKTY MYHVRLIGGN RIYVAEMNDD LRSMKEETAR
     ECIAVNDKGW ENTAEGKWGV SEGPTVVKLD GTYYMFYSCN DFRSIDYAMG YATAKSPLGP
     WKKHKKPIVS RHLTGENGTG HGDLFRDGDG RWMYVLHTHN SNSKVSPRRT AMVELVKKGK
     KFEMVPGSLR YVTR
//