ID R5R4D1_9FIRM Unreviewed; 982 AA.
AC R5R4D1;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 39.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00013303, ECO:0000256|RuleBase:RU361154};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN ORFNames=BN747_01159 {ECO:0000313|EMBL:CCZ36030.1};
OS Firmicutes bacterium CAG:646.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1262995 {ECO:0000313|EMBL:CCZ36030.1, ECO:0000313|Proteomes:UP000018386};
RN [1] {ECO:0000313|EMBL:CCZ36030.1, ECO:0000313|Proteomes:UP000018386}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:646 {ECO:0000313|Proteomes:UP000018386};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU361154};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ36030.1}.
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DR EMBL; CAZW010000199; CCZ36030.1; -; Genomic_DNA.
DR AlphaFoldDB; R5R4D1; -.
DR STRING; 1262995.BN747_01159; -.
DR Proteomes; UP000018386; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154};
KW Reference proteome {ECO:0000313|Proteomes:UP000018386}.
FT DOMAIN 703..982
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
SQ SEQUENCE 982 AA; 113569 MW; F0055276D036CB42 CRC64;
MRSSEWQNPK FLQKGRETAR AHFIPCRTME EALSGKVESS SRYQSLNGTW DFKYFEAWYL
MEEPVHFTES IPVPSNWQMH GYDIPYYTNV NYPYPADMPR VPDDNPCGVY RRYFKVQHPE
DEVYLNFEGV NSCFYVYING QEIGYSQGSH YTAEFCITPY LREGNNELLV KVLKWCDGSY
LEDQDFFRLS GIFRDVYLLY RNPSHVRDLE INTDLHNLTV KLDVTGEIEG YTIGLYEGAT
LLEEQAATRE GAEFVLENPR LWTAETPELY TVVISGHGEV IARKAGFRTV EVSEKGELLI
NGTPVKLKGV NHHDTHPEKG HVMDEEDMKK DLYLMKRLNI NCVRTSHYPP AARFLELCDE
VGMYVVDEAD LECHGLVTKD TMWEYGLFYK DWPTDHPDWK EAMMERAVRM VERDKNFPCI
IVWSMGNEAG YGQYFEDMAV WTKERDASRL VHYERANMAD NPPCIDVESG MYYSLDMVKE
EGEKDSKRPF FLCEYAHAMG NGPGGLGDYW ELFEKYPRLI GGCIWEWADH VAVKEGRHLY
GGDFGEITHD HNFCVDGLVM ADRSLKAGSR EAKAVHQPLR AELISAKPLT VKIINHFDFA
TFKDYEILWT VEIDGKTAFS GYWEGDLAAG ESIEVVLSGE LPSSCELGAY LNLSLVRKTS
CSWAEAGYET ALVQLELPVG KQKKQREVQE KTWEIQEDSI FLKITDEQEN GYQFHKIKGI
LTGIFKNGQN LLFSPTQLSL WRAPTDNERH MKTKWGLFED NISGWNLNYL MDKCYSMEWE
QKDHTVCVSV EGALAGISRT PSSWYKICYQ ICADGSLEVK VHAKMSKNKN AIWLPRFGFE
FRVPYQMEQM EYFGRGPWEN YCDLYHHAPV GLYTSNASRE YFPYVKPQEN GNHTKVKYLT
VKNPHTGETL TFEAEKEMEC QVLHYTKEEL TEKKHYFELE ENETTIRIDY KNSGIGSASC
GPELEEKDRL SEKEIEFAFT VR
//
