ID R5UHE4_9BACE Unreviewed; 870 AA.
AC R5UHE4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=BN535_01329 {ECO:0000313|EMBL:CCZ75119.1};
OS Bacteroides caccae CAG:21.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=1263037 {ECO:0000313|EMBL:CCZ75119.1, ECO:0000313|Proteomes:UP000017990};
RN [1] {ECO:0000313|EMBL:CCZ75119.1, ECO:0000313|Proteomes:UP000017990}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:21 {ECO:0000313|Proteomes:UP000017990};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ75119.1}.
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DR EMBL; CBAN010000207; CCZ75119.1; -; Genomic_DNA.
DR AlphaFoldDB; R5UHE4; -.
DR Proteomes; UP000017990; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 3.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..870
FT /note="Beta-mannosidase B"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004406031"
FT DOMAIN 222..331
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 689..787
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 790..866
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 870 AA; 100315 MW; 104B03166A40DB57 CRC64;
MILNLTGKKA QIACGLLCCC SMAYAQSNDN SEVVLLHTGW EFSQAGTELW RTAEVPGTVH
QDLIHHKLLP SPFYGMNEQK IQWVENEDWE YRTSFTVTME QLERDDAQLI FEGLDTYADV
YLNGSLLLKS DNMFVGYSLP VKPQLRLGEN LLHIYFHSPI RQTLPQYASN GFNYPADNDH
HEKHLSVFSR KAPYSYGWDW GIRMVTSGIW RPVKIRFYDA ASINDYHIKQ LSLTEHSAEL
SNELEINNIL PQSIQAEIRI NYSFEKGEET VISRSVTLQP GLNSIHIPSE VLSPVRWMPN
GWGKPALYDF SAQVVFDGKV VAEQSHRIGL RTVRLVNEKD ADGESFYFEV NGIPMFAKGA
NYIPQDALLP SVTTERYQTL FRDIKEANMN VVRIWGGGTY EDDRFYDLAD ENGILVWQDF
MFACTPYPSD PTFLKRVEEE ACYNIRRLRN HPSLAMWCGN NEILEALKYW GYQKKYTPEI
YQEMMSGYDK LFRELLPAKV KELDADRFYI HSSPYLANWG RPESWGIGDS HNWGVWYGQK
PFESLDTDLP RFMSEFGFQS FPEMKTIATF AAPGDYQIES EVMNAHQKSS IGNALIRTYM
ERDYIVPEKF EDFVYVGLVL QGQGMRHGLE AHRRNRPYCM GTLYWQLNDS WPVVSWSSID
YYGNWKALHY QAKRAFAPIH INPIQRNDSL CVYLLSDRLD TLEKMTLEMK VIDFEGKKIS
KPMLLKSLSV PANTSQCVYR TKLDALLSST KRPLSEELLH CFMLLTLKDK SGHVVDETVY
FFAKTKDLLL PETTISYKMK QTDGECELTL LSPVLAKDVF IEVPLQGARF SDNFFDLLPG
ERKTILITSP QIKKGEELPL TVKHIRETYN
//
