ID R5VT67_9FIRM Unreviewed; 625 AA.
AC R5VT67;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755};
GN ORFNames=BN566_01467 {ECO:0000313|EMBL:CCZ83732.1};
OS Ruminococcus sp. CAG:254.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Ruminococcus.
OX NCBI_TaxID=1262953 {ECO:0000313|EMBL:CCZ83732.1, ECO:0000313|Proteomes:UP000018181};
RN [1] {ECO:0000313|EMBL:CCZ83732.1, ECO:0000313|Proteomes:UP000018181}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:254 {ECO:0000313|Proteomes:UP000018181};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCZ83732.1}.
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DR EMBL; CBAR010000159; CCZ83732.1; -; Genomic_DNA.
DR AlphaFoldDB; R5VT67; -.
DR STRING; 1262953.BN566_01467; -.
DR Proteomes; UP000018181; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000018181}.
FT DOMAIN 1..183
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 547..622
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
SQ SEQUENCE 625 AA; 71028 MW; 67EA1837200C883B CRC64;
MRYQSYSIHK GKYAIAGLPA VYADEDEAET LEIVLKDTAT DLQVTLRYGV LPELDMLTRC
VSIQNLGTTP ITLTKAASFC LDLPYGKWEW MHFHGRHAME RITERTPLIH GIQESSSTRG
TSSHHQNPTA ILCTPDCTET NGSCFGAAFL YSGSFQTKIE YDQMRQARMV MGLHPDLFRW
ELQPNATFDT PEVLFTYSDS GLETLSHRFQ KTIREHVCRG KYQKIERPVL INNWEATYFD
FNEEKILKIA EEAARLGVDM LVLDDGWFGK RDDDCSGLGD WFVNEQKLRG GLGTLVQKVK
ALGMRFGIWF EPEMISEDSD LYRTHPDWAI QIPGRNPMRS RYQLLLDLSN PDVQDYLYKC
ISDILNSADI SYVKWDMNRS ISDWYTALLP ANRQGELPHR YVLGVYALLE RLTSAFPEVL
FEGCSGGGGR FDAGMLYYCP QIWCSDDTDA YERTIIQYGT SFFYPVSAVG SHVSVVPNHQ
TGRVTPIETR AVTAMAGSFG YELDLNLLSD EEKEAVTQQI QQFKEYGSLI HNGTYYRLSN
PLEDAYALWA FVSEDKKEVL VQGMIFRTEA NMVRHCVSLR GLDAKAVYRT KDGAAYTGDT
LMHAGVLLPK SWGDYYPVSM HFVSE
//
