ID   R5YBI4_9FIRM            Unreviewed;       497 AA.
AC   R5YBI4;
DT   24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT   24-JUL-2013, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   SubName: Full=Glutamate synthase (NADH) small subunit {ECO:0000313|EMBL:CDA24918.1};
GN   ORFNames=BN528_01722 {ECO:0000313|EMBL:CDA24918.1};
OS   Roseburia sp. CAG:197.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia.
OX   NCBI_TaxID=1262943 {ECO:0000313|EMBL:CDA24918.1, ECO:0000313|Proteomes:UP000018336};
RN   [1] {ECO:0000313|EMBL:CDA24918.1, ECO:0000313|Proteomes:UP000018336}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MGS:197 {ECO:0000313|Proteomes:UP000018336};
RA   Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA   Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA   Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA   Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA   Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA   Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA   Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA   Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA   Wang J., Brunak S., Ehrlich S.D.;
RT   "Dependencies among metagenomic species, viruses, plasmids and units of
RT   genetic variation.";
RL   Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Amino-acid biosynthesis. {ECO:0000256|ARBA:ARBA00029440}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDA24918.1}.
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DR   EMBL; CBBL010000150; CDA24918.1; -; Genomic_DNA.
DR   AlphaFoldDB; R5YBI4; -.
DR   Proteomes; UP000018336; Unassembled WGS sequence.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0016639; F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   GO; GO:0006537; P:glutamate biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.1060.10; Alpha-helical ferredoxin; 1.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   InterPro; IPR028261; DPD_II.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR023753; FAD/NAD-binding_dom.
DR   InterPro; IPR006005; Glut_synth_ssu1.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   NCBIfam; TIGR01317; GOGAT_sm_gam; 1.
DR   PANTHER; PTHR43100; GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN; 1.
DR   PANTHER; PTHR43100:SF1; GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN; 1.
DR   Pfam; PF14691; Fer4_20; 1.
DR   Pfam; PF07992; Pyr_redox_2; 2.
DR   PRINTS; PR00419; ADXRDTASE.
DR   SUPFAM; SSF46548; alpha-helical ferredoxin; 1.
DR   SUPFAM; SSF51971; Nucleotide-binding domain; 2.
PE   4: Predicted;
KW   Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW   Glutamate biosynthesis {ECO:0000256|ARBA:ARBA00023164};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000018336}.
FT   DOMAIN          23..139
FT                   /note="Dihydroprymidine dehydrogenase"
FT                   /evidence="ECO:0000259|Pfam:PF14691"
FT   DOMAIN          153..326
FT                   /note="FAD/NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF07992"
FT   DOMAIN          387..477
FT                   /note="FAD/NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF07992"
SQ   SEQUENCE   497 AA;  54760 MW;  FFB27397F553FAC1 CRC64;
     MGKPTGFLEY ERTEARSVEP KERIKNYKEF HVFLSKEKQQ EQAARCMDCG VPFCQSGMTI
     AGMTSGCPLH NLVPEWNDLV YSGNWQQAYN RLHKTNNFPE FTSRVCPALC EKACTCGHWG
     DPVSVRDNEH GVIETAYERG YAAPHVPKVR TGKKVAIIGS GPSGLAAADQ LNQRGHDVTV
     YERDDRVGGL LMYGIPNMKL EKHIIDRKID VMKAEGIHFI TGCNVGVDVK AADLLKQYDR
     VVLCCGAKNP RDIKAAGRDA EGIYFAVDYL SRTTKSLLDS GLKDNKYVSA KGKNVVIIGG
     GDTGNDCVGT AIRQGAKSVT QLEMMPCPPT ERAANNPWPE WPKVLKTDYG QEEAIACFGT
     DPRIYQTTVK EFHKDKSGKL NGLTIVSLES KVDEKTGRRN MVEVPGTEKK IPAELVLIAA
     GFLGTEDYIA KAFGVELNQR TNVATEPGKY ATNVKNVFTA GDMHRGQSLV VWAIREGREV
     AHDVDASLMG YSYLSVQ
//