ID R6APN4_9FIRM Unreviewed; 1140 AA.
AC R6APN4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=BN678_00509 {ECO:0000313|EMBL:CDA50674.1};
OS Dialister sp. CAG:486.
OC Bacteria; Bacillota; Negativicutes; Veillonellales; Veillonellaceae;
OC Dialister; environmental samples.
OX NCBI_TaxID=1262870 {ECO:0000313|EMBL:CDA50674.1, ECO:0000313|Proteomes:UP000017985};
RN [1] {ECO:0000313|EMBL:CDA50674.1, ECO:0000313|Proteomes:UP000017985}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:486 {ECO:0000313|Proteomes:UP000017985};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC responsible for most of the replicative synthesis in bacteria. This DNA
CC polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC is the DNA polymerase. {ECO:0000256|ARBA:ARBA00025611}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the POLIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III
CC complex. {ECO:0000256|ARBA:ARBA00026073}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC subfamily. {ECO:0000256|ARBA:ARBA00009496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA50674.1}.
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DR EMBL; CBBY010000276; CDA50674.1; -; Genomic_DNA.
DR AlphaFoldDB; R6APN4; -.
DR STRING; 1262870.BN678_00509; -.
DR Proteomes; UP000017985; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF160975; AF1531-like; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000017985};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..72
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1140 AA; 127450 MW; 403EE173336BA58D CRC64;
MDPFVHLHVH TEYSLFDGTC RIKELVSYVK SLGQTAVAIT DHGVMYGAVY LYKECIAQGI
KPIIGCEVYV TRKSRFERAK GEKEKLGHLI LLAETNEGYQ NLIKICSKAW TEGYFHKPRV
DHELLSKYHK GLIALSACVA GELPQAILNR DMDEVRKVIR YYIDTFGKEN YFLELQNHGI
PEEADVRPVL ASLAKEYGLG LVATNDFHYT KKEDAKSQEI KLCISTGKTL DDPYHFHFPN
DEFYCKSGEE MQAILGEYPG AIENTAKIAE RCHVTFTFGN HKLPSFDVPE GETSKSYLRK
LCEAAIPERY PESTGKEQER LDYELGVINQ MGFSDYFLIV MDFIRYAKTH GIPIGPGRGS
AAGSIVAYLL HITEIDPLRF DLLFERFLNP ERVSMPDIDT DLCYRGRGQV IAYLAEKYGA
EQVAQIITFG TLAARAVIRD VGRVMNLPLR EVDKIAKMVP SGPGVTLKKT LDGSKEFKDL
YESNATVRTL IDHCLDLEGL SRNSGTHAAG VVICSKPVDE YVPIQLTADD FIQTQYEKDQ
VEQLGLLKMD LLGLRNLTVI HDAVEMIRAN RGVTIDIDRI PSVDEKTCEM LCAGDTTGVF
QSESSGFTNL LMKLHPDRFE DLIPMVALYR PGPLGSGMAE DFIKRKHGEI PIEYPHPSLA
PILKETYGVI LYQEQVMQIA SVMGGFSLGQ SDMLRRAMGH KEPEILQKNR DTFVTGAVKN
GVDEKTANYV FDLMVHFAGY GFNKSHSVCY GWIAWQTAYL KAHYRAEFMA AMMTCYNGDK
NKVSRYISDT RRAGVEIAAP DVNLSEAYFS VSGNKILFGL DAIQNVGENA VEAIIKARKK
GGPFTSMSDF LERVEGKGLN SRACESLIRC GAMDGFGYNR SQLLAALPQV MSDVAASKSD
RESGQMSLFG GDEMKSSVVY PDLEDMDADE KIDWERKLLG FYVSGHPLEK YKKQMAACTP
IYQLETEGSR YDGKTVTLGG TVTRVKGMVT KKGAPMGYVT IEDYESEIES VLFPSVWEAA
RPFLTEDAPI CIRGRVQSNE REVKILADSI IPMAQFQKTV RMPVDEVHLY VDPRHETDKV
SAALARVLAK YHGGTPVILH LISSRQEIRM VPKFYVDFSE EAEEALKGLL GNAAVEGRKN
//
