ID R6E273_9FIRM Unreviewed; 1129 AA.
AC R6E273;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BN553_01634 {ECO:0000313|EMBL:CDA90661.1};
OS Firmicutes bacterium CAG:238.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1263011 {ECO:0000313|EMBL:CDA90661.1, ECO:0000313|Proteomes:UP000018260};
RN [1] {ECO:0000313|EMBL:CDA90661.1, ECO:0000313|Proteomes:UP000018260}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:238 {ECO:0000313|Proteomes:UP000018260};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA90661.1}.
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DR EMBL; CBCS010000086; CDA90661.1; -; Genomic_DNA.
DR AlphaFoldDB; R6E273; -.
DR Proteomes; UP000018260; Unassembled WGS sequence.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR018982; RQC_domain.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11070:SF63; DNA HELICASE IV; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF09382; RQC; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000018260}.
FT DOMAIN 135..617
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT REGION 963..985
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 156..163
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1129 AA; 131204 MW; C6391057B35B7B0B CRC64;
MSIFSKLFKK IPKDSETIGN RKDFSNDQCR KLWELKKFFE ELFAKEDYIA KSEYKTAVMN
YEEVIRYFDV LKNSGLLGTF CEQNDFPLAV AMTILSNYEN IENLVDAHNE AIVRKLMIEE
KQYLDNILKD VDSNILLDDD QRKVILTDED YMLVIAGAGA GKTTTVAAKV KYLVEKKHID
PKQILVVSFT NKAVQELRDK LNKDLNIDCP IATFHSTGNA ISRKQSPEKL NIVDSSKLYF
VLQDYFKSSV MKNESIANSL ILFFASYFEA PYNGTDLNSF FNKIAKSNFS TMRSDLDEFK
QEIVDIRTKK KVTIQSEVMR SYQEVEIANF LYLNGIDYQY EPIYKYDILH ARKPYTPDFV
IMQDGKTAYI EHFGLTEDGK NDKYSENEIE YYKKAVKDKI QLHRKHETTL IYTFSKYNDG
RSLQEHLKEK LEAGGFILKP RSNKEVMKKI VESEENRYLR KLINLICRFI TNFKTNGFTE
GDFARMYNST QNVRTRLFLN ICHDCYLEYQ RFLRENEAVD FEDMINESAR ILQETKEMKQ
KLDFKYIIVD EYQDISRQRF DLVKALAEVT DAKIIAVGDD WQSIYAFSGS DITLFTKFAE
KMGYAKLLKI VKTYRNSQEV IDIAGNFIQK NSSQIKKALK SPKHIADPVI IYTYDSTKKS
ANASARSGAN YAAAHAVEIA IEQIMEFDRI EGKKTSNILV LGRFGFDGDK LEKSGLFEYK
TRGGKLKSVK YPKLDLTFMT AHASKGLGYD NVIVINGRNE TYGFPSKIED DPVLSFVVKQ
DRSIEYAEER RLFYVAMTRT KNRVYFIAPE QNPSEFLLEL KRDYKNIVLK GDWNEKSTQS
QLTNKSCPMC GYPMQYRYKN AYGLRLYICT NEPEICGFMT NEYRAGKLSI IKCDQCRDGY
LVVKQGKETY VLGCTNYKKD GTGCSRVMSP KYFFDFMKIK EEKMPLIPKY ECRSKDKIVR
KSEEKKSYNE SSKAQGDKEK PSKQAEIKEI PKLDIKPIIY NGWNLNEVVH TVLQALSHMS
EIRFYGVQVL LHILIGQTIK KVDKESLMTI PEFGMLKDLD KNEIEFIIKW LINNQFILQT
KHAYYPLLHP TYAGTHYNET MTDRKLKKLK DELIMYNSID KNRKSGIIK
//
