ID R6E796_9FIRM Unreviewed; 1022 AA.
AC R6E796;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=BN553_00710 {ECO:0000313|EMBL:CDA92466.1};
OS Firmicutes bacterium CAG:238.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1263011 {ECO:0000313|EMBL:CDA92466.1, ECO:0000313|Proteomes:UP000018260};
RN [1] {ECO:0000313|EMBL:CDA92466.1, ECO:0000313|Proteomes:UP000018260}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:238 {ECO:0000313|Proteomes:UP000018260};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the POLIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III
CC complex. {ECO:0000256|ARBA:ARBA00026073}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA92466.1}.
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DR EMBL; CBCS010000215; CDA92466.1; -; Genomic_DNA.
DR AlphaFoldDB; R6E796; -.
DR Proteomes; UP000018260; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000018260};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 95..376
FT /note="Bacterial DNA polymerase III alpha subunit NTPase"
FT /evidence="ECO:0000259|Pfam:PF07733"
FT DOMAIN 379..539
FT /note="DNA polymerase III alpha subunit finger"
FT /evidence="ECO:0000259|Pfam:PF17657"
FT DOMAIN 627..715
FT /note="DNA polymerase helix-hairpin-helix motif"
FT /evidence="ECO:0000259|Pfam:PF14579"
FT DOMAIN 827..900
FT /note="OB"
FT /evidence="ECO:0000259|Pfam:PF01336"
SQ SEQUENCE 1022 AA; 114738 MW; 4940C405BC0C71AD CRC64;
MATNDAHYVR REDAKAHDVL LAIQTATTLD DEKRMRFPND EFYLKSEDEM RKIFAYCPEA
CDNTQKIAEQ CNYDFRFGEY HLPEFQAPLG KTNTEYLREL CYAGLEDRFG VVFEEGKLGD
ADAARYADGT EYSTVHTPKE LAERLNYELG TIESMGYIEY FLIVWDFINY AKSHEIMVGP
GRGSAAGSIV AYCLKITDID PIRYNLIFER FLNPERVSMP DIDIDFCYER RQEVIDYVTE
KYGEGRVSQI ITFGTMKAKQ AVRDVGRALS VSYAKTDQIA KAIPFELGMT IDKALATSPD
LKNLYENDEE TREVIDMAKA IEGMPRHAST HAAGVVISKA PIDEYVPLYL SEKGPATQFT
MTTIEELGLL KMDFLGLRNL TVIRDALELI KKNHGVSIDF ARMGYDDPSV YEMISAGNTS
GVFQLESGGM TQFMKNLRPT CFEDVVAGIS LYRPGPMDSI PKYIENKKNP SGIQYVDESL
APILDVTYGC LVYQEQVMQI VRDLGGYSYG RSDLVRRAMS KKKKDVMLQE KEYFINGKLD
ENGNVEIAGC IRNGISRKAA ETIFEDMETF AQYAFNKSHA AAYAVVAYET GYLKKHYPVE
FMAALMTSVM GDAKMIARYI RNCSDMGIEV LPPSVNKSLK KFSVEDGKIR FGLLGVKNVG
ENVIEAILKA REEKGEPSDI FTFINNLDIS QVNKRAIESL IKAGACDCLE GNRAAKLAVY
ETLVESAQNT SKKNLEGQIS LFQMASDAMS SAGTGKRLPD VQAFPLNIEL AMEKEMLGVY
LTGHPLDAYK EKMKRISSVT SDDLMQIEEE SGTAQNHAIR DGMSCIISGM IAGKKTLITK
SNKMMAFIDL EDLYGITEVV IFPNVYEKCA EEIQDDRVVA IKGTLNFKED EAPKVLADEV
LDIDTAIERG FTQRSRNGYN GSRGKNYGTK LMAAVSQQKT EASEPTRQPA GMVKLRIPAD
MDEAMTLEEI KVNLKRHEGD HEVRIYLPSG KMFRTEESLW VSPSEELRKQ MAAILRMENV
KM
//
