ID R6EA11_9CLOT Unreviewed; 246 AA.
AC R6EA11;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Ribosomal RNA small subunit methyltransferase E {ECO:0000256|PIRNR:PIRNR015601};
DE EC=2.1.1.193 {ECO:0000256|PIRNR:PIRNR015601};
GN ORFNames=BN547_01603 {ECO:0000313|EMBL:CDA87032.1};
OS Clostridium sp. CAG:230.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1262782 {ECO:0000313|EMBL:CDA87032.1, ECO:0000313|Proteomes:UP000017961};
RN [1] {ECO:0000313|EMBL:CDA87032.1, ECO:0000313|Proteomes:UP000017961}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:230 {ECO:0000313|Proteomes:UP000017961};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Specifically methylates the N3 position of the uracil ring of
CC uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S
CC ribosomal subunit. {ECO:0000256|ARBA:ARBA00025699,
CC ECO:0000256|PIRNR:PIRNR015601}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(1498) in 16S rRNA = H(+) +
CC N(3)-methyluridine(1498) in 16S rRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:42920, Rhea:RHEA-COMP:10283, Rhea:RHEA-COMP:10284,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74502; EC=2.1.1.193;
CC Evidence={ECO:0000256|ARBA:ARBA00000792,
CC ECO:0000256|PIRNR:PIRNR015601};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|PIRNR:PIRNR015601}.
CC -!- SIMILARITY: Belongs to the RNA methyltransferase RsmE family.
CC {ECO:0000256|ARBA:ARBA00005528, ECO:0000256|PIRNR:PIRNR015601}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA87032.1}.
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DR EMBL; CBCQ010000123; CDA87032.1; -; Genomic_DNA.
DR AlphaFoldDB; R6EA11; -.
DR STRING; 1262782.BN547_01603; -.
DR Proteomes; UP000017961; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR CDD; cd18084; RsmE-like; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR006700; RsmE.
DR InterPro; IPR046886; RsmE_MTase_dom.
DR InterPro; IPR046887; RsmE_PUA-like.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR NCBIfam; TIGR00046; RsmE family RNA methyltransferase; 1.
DR PANTHER; PTHR30027:SF3; 16S RRNA (URACIL(1498)-N(3))-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR30027; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE E; 1.
DR Pfam; PF04452; Methyltrans_RNA; 1.
DR Pfam; PF20260; PUA_4; 1.
DR PIRSF; PIRSF015601; MTase_slr0722; 1.
DR SUPFAM; SSF75217; alpha/beta knot; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR015601};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|PIRNR:PIRNR015601};
KW Reference proteome {ECO:0000313|Proteomes:UP000017961};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552,
KW ECO:0000256|PIRNR:PIRNR015601};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PIRNR:PIRNR015601};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR015601}.
FT DOMAIN 18..64
FT /note="Ribosomal RNA small subunit methyltransferase E PUA-
FT like"
FT /evidence="ECO:0000259|Pfam:PF20260"
FT DOMAIN 73..241
FT /note="Ribosomal RNA small subunit methyltransferase E
FT methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF04452"
SQ SEQUENCE 246 AA; 27742 MW; 28653C2A8F956424 CRC64;
MFRFYITDEQ ICNDEIFIEG GDVNHIRNVL RLEKGDWVVA CNGEGKDYVS RIASIEKEQV
VLQVEKVQET GTELPTKITL FQGLPKKDKM EFIIQKAVEL GVYEIVPVMM KRCVVKLGDE
KKIQKKQERW QAIAEAAAKQ CDRGIIPVVH APITMKEAIE KAKELSYNVI PYELQEGIDH
SREIVAHACK QESVGIFIGP EGGFEDSEVE LAIANGMEAI TLGKRILRTE TAGMALLSIM
MFQMQQ
//
