ID R6EBT4_9BACT Unreviewed; 453 AA.
AC R6EBT4;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Glycosyl hydrolase family 43 {ECO:0000313|EMBL:CDA94764.1};
GN ORFNames=BN487_01125 {ECO:0000313|EMBL:CDA94764.1};
OS Prevotella sp. CAG:1320.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Prevotella.
OX NCBI_TaxID=1262922 {ECO:0000313|EMBL:CDA94764.1, ECO:0000313|Proteomes:UP000018111};
RN [1] {ECO:0000313|EMBL:CDA94764.1, ECO:0000313|Proteomes:UP000018111}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:1320 {ECO:0000313|Proteomes:UP000018111};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDA94764.1}.
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DR EMBL; CBCU010000053; CDA94764.1; -; Genomic_DNA.
DR AlphaFoldDB; R6EBT4; -.
DR STRING; 1262922.BN487_01125; -.
DR Proteomes; UP000018111; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04084; CBM6_xylanase-like; 1.
DR CDD; cd18618; GH43_Xsa43E-like; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR006584; Cellulose-bd_IV.
DR InterPro; IPR005084; CMB_fam6.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43772:SF6; -; 1.
DR PANTHER; PTHR43772; ENDO-1,4-BETA-XYLANASE; 1.
DR Pfam; PF03422; CBM_6; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SMART; SM00606; CBD_IV; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS51175; CBM6; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00022651};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00022651};
KW Reference proteome {ECO:0000313|Proteomes:UP000018111};
KW Signal {ECO:0000256|SAM:SignalP};
KW Xylan degradation {ECO:0000256|ARBA:ARBA00022651}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..453
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004404463"
FT DOMAIN 324..450
FT /note="CBM6"
FT /evidence="ECO:0000259|PROSITE:PS51175"
FT ACT_SITE 32
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 209
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 147
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 453 AA; 50474 MW; ADF6BDBD48356BB9 CRC64;
MLRHKILCSL LAMSPLAGMA QNPIVQTMYT ADPAPMVSGD RLFLYTSHDE DNSTWFVMNN
WKLYSTTDMV NWTDHGVVLS LKTFDWAQSD AWAMQCVERD GKYYAYVPVT KKGGGGAIGV
AVADSPYGPF YDPLGKPLVS SGRGDIDPSV MIDEDGQAYL YWGNPFCYYV KLNEDMISYS
GDIVRVPMTE EAFGKREGNV EERPTRYEEG PWLYRHDKWY YLLWAGGPIS EHLGYSMSKS
PTGPWKNCGT LMPTEGRSFT NHPGIVDYKG NTYLFYHNGA LPGGGGFTRS VCVEQAEFNK
DGTIKPMKMT AGITKGLGTL NPYRKTEAET MAFSEGMKGA QNEEVGVYVN AMKDGAYVKV
RDVDFREAGP AKVTARVGTT HNGGVTMEVR VDNPQGPLLA TLKVPMTGWD DRWAVVSADV
SKVSGVHDLY FVCRGDKPGR LMYFDYWMFS QNK
//
