ID R6EV50_9BACT Unreviewed; 785 AA.
AC R6EV50;
DT 24-JUL-2013, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2013, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=BN691_01390 {ECO:0000313|EMBL:CDB04528.1};
OS Prevotella sp. CAG:520.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Prevotellaceae;
OC Prevotella.
OX NCBI_TaxID=1262929 {ECO:0000313|EMBL:CDB04528.1, ECO:0000313|Proteomes:UP000018359};
RN [1] {ECO:0000313|EMBL:CDB04528.1, ECO:0000313|Proteomes:UP000018359}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MGS:520 {ECO:0000313|Proteomes:UP000018359};
RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., Sunagawa S.,
RA Plichta D., Gautier L., Le Chatelier E., Peletier E., Bonde I., Nielsen T.,
RA Manichanh C., Arumugam M., Batto J., Santos M.B.Q.D., Blom N., Borruel N.,
RA Burgdorf K.S., Boumezbeur F., Casellas F., Dore J., Guarner F., Hansen T.,
RA Hildebrand F., Kaas R.S., Kennedy S., Kristiansen K., Kultima J.R.,
RA Leonard P., Levenez F., Lund O., Moumen B., Le Paslier D., Pons N.,
RA Pedersen O., Prifti E., Qin J., Raes J., Tap J., Tims S., Ussery D.W.,
RA Yamada T., MetaHit consortium, Renault P., Sicheritz-Ponten T., Bork P.,
RA Wang J., Brunak S., Ehrlich S.D.;
RT "Dependencies among metagenomic species, viruses, plasmids and units of
RT genetic variation.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CDB04528.1}.
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DR EMBL; CBCY010000061; CDB04528.1; -; Genomic_DNA.
DR AlphaFoldDB; R6EV50; -.
DR STRING; 1262929.BN691_01390; -.
DR Proteomes; UP000018359; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000018359}.
FT DOMAIN 1..284
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 285..573
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 659..700
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 20..27
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 785 AA; 88907 MW; 99DF6BFBBFD5F17C CRC64;
MNPNQLAAVE YTDGPELIIA GAGSGKTRVL TYKIAYLLQK GLAPWSILAL TFTNKAAREM
KSRVAELVGE DRARYLNMGT FHSVFLRILR AEAEAVGLQR NFSIYDDGDS RGVVNSIIKE
MQLDDKQYKA SAILGRIGAA KNSLVTPEVY MSDPHLLERD KVSSMPKLGQ IYAMYAARCK
AANAVDFDDM LLFTYKLFAE NPQIAKKYAE RFCYVLVDEY QDTNYAQQAI LTLLTKEQRN
ICVVGDDAQS IYAFRGANID NILKFQSIYE GARLFKLEQN YRSTQTIVKA ANSVIVNNQR
QIRKEVFSEN GDGEKVHLNI AYSDKEEASV VCNEIRRLHN RYGVAYRDIA VLYRTNAQSR
LFEEELRRQD TPYRIYGGMS FYQHKEIKDV IAYFRLVANP NDEEALKRVI NYPKRGIGDT
TISKLIAASR ACNVSLWDVV GNPSMYNASV SAGTEKKVLG FYYLIDEFIR DNRTVDAYGL
GKEILEKSGL KFELASDKTM EGAERQENIE EFMSGLHDFV DTKREEGYED EVYIANFLQE
VALLTDSDEE NDNDNDASSN ERVSLMTIHA SKGLEFNSVF VVGLEENIFP SPRACSSVRE
LEEERRLLYV AITRAEQRCF LSCAKSRYQY GSMMFNEPSR FLKEIDKQYL VIEGNAAGFK
SQGSQPQPFQ SRPQSRSFQS RPQSQPYHET PQRNPFQSTV PSGFRRVQSV SQRFQTSASI
QRMQASASSQ RQTIHAGARV EHQRFGLGTV VAVEGVGENE KATIEFQNAG TKTLLMKFAR
LTVLK
//
